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Genome SequencesDraft Genome Sequence of an Endophytic Biocontrol Bacterium, Bacillus velezensis PG12, Isolated from Apple FruitBacillus velezensis PG12 is a biocontrol endophytic bacterium that is capable of inhibition of a broad range of plant-pathogenic fungi. The strain has the potential to be developed into a biocontrol agent for use in agriculture. Here, we report the draft genome sequence of B. velezensis PG12, which contains 22 scaffolds (3,990,845 bp), 3,884 coding sequences...
Other Genetic ResourcesA New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction EnzymesA series of new Escherichia coli entry vectors (pIIS18-SapI, pIIS18-BsmBI, pIIS18-BsaI, pIIS18-BfuAI-1, and pIIS18-BfuAI-2) was constructed based on a modified pUC18 backbone, which carried newly designed multiple cloning sites, consisting of two facing type IIS enzyme cleavage sites and one blunt-end enzyme cleavage site. These vectors are useful for seamless gene...
Genome SequencesComplete Genome Sequence of Vibrio natriegens Phage PhriendlyVibrio natriegens is a fast-growing marine bacterium of interest for biotechnology applications. Here, we describe the isolation and genome annotation of V. natriegens podophage Phriendly. Among the 72 predicted coding genes and 1 tRNA, Phriendly’s 50,218-bp genome has low sequence identity to known phages.
Genome SequencesDraft Genome Sequence of Kytococcus schroeteri Strain H01, Isolated from Human SkinKytococcus schroeteri strain H01 was isolated from the skin of a healthy volunteer who underwent erythromycin treatment for a skin disorder 1 year prior. The draft genome consists of 2.38 Mb, a G+C content of 73.06%, and 2,221 protein coding sequences. This is the first genome characterization of a K....
Genome SequencesWhole-Genome Sequencing of a Mycobacterium orygis Strain Isolated from Cattle in Chennai, IndiaHere, we report the isolation of Mycobacterium orygis from dairy cattle in Chennai, India. Spoligotyping assigned the isolate to spoligotype 587 (ST587), which belongs to M. orygis. This species was confirmed as M. orygis using whole-genome sequencing.
Genome SequencesComplete Genome Sequence of a Papio anubis Simian Foamy ProvirusThe full-length sequence of a Papio anubis simian foamy provirus was obtained by using PCR followed by Sanger sequencing. This simian foamy virus from a P. anubis animal (SFVp.anubis) is 13,393 bp long. Like other proviruses, the genome of SFVp.anubis is organized with long terminal repeats (LTRs),...
Genome SequencesComplete Genome Sequence of Escherichia coli Siphophage SnokeEscherichia coli is a Gram-negative bacterium often found in animal intestinal tracts. Here, we present the genome of the Guernseyvirinae-like E. coli 4s siphophage Snoke. The 44.4-kb genome contains 81 protein-coding genes, for which 33 functions were predicted. The capsid morphogenesis...
Genome SequencesComplete Genome Sequence of Staphylococcus aureus Myophage MaineMultidrug-resistant strains of Staphylococcus aureus cause serious human disease worldwide. Bacteriophages offer a promising alternative to traditional antibiotics. Here, we announce the 141,712-bp genome of S. aureus phage Maine. A myophage with 9,019-bp predicted terminal repeats and high...
Genome SequencesComplete Genome Sequence of Bacteriophage Finny, Isolated from a Microbacterium foliorum CultureActinobacteriophage Finny contains a circularly permuted 40,313-bp double-stranded DNA genome with 63 predicted protein-coding genes. Finny was directly isolated from a soil sample collected in New Braunfels, Texas, that was incubated with Microbacterium foliorum SEA B-24224. Finny is closely related to bacteriophages MCubed, Andromedas, ColaCorta, Eleri, and Sansa....
Genome SequencesComplete Genome Sequence of a Quorum-Sensing Bacterium, Oceanicola sp. Strain D3, Isolated from a Microplastic Surface in Coastal Water of Qingdao, ChinaOceanicola sp. strain D3 was isolated from the plastisphere of polyvinyl chloride (PVC) in coastal water of Qingdao, China. Here, we present the complete genome sequence of strain D3, which consists of a chromosome of 3,926,685 bp with a G+C content of 64.49% and 4,964 coding DNA sequences. This is the first report of a quorum-sensing (QS) system in an Oceanicola...