TABLE 1

Summary of 16S rRNA gene amplicon profiles of the isolated microbiota

Analysis measureData by sample type (DRR accession no.)a
Bulking sludge A (DRR180051DRR180056)Bulking sludge B (DRR180045DRR180050)Healthy granular sludge A (DRR180039DRR180044)Healthy granular sludge B (DRR180033DRR180038)
Sampling date27 Feb 20147 Feb 201817 Dec 201427 Nov 2017
Diversity
    Estimated sample coverage0.980.980.980.98
    No. of OTUs554.7554.3323.8582.3
    Shannon diversity6.416.155.006.07
    Simpson diversity0.970.960.920.95
    Chao1 estimator837.8947.1531.3932.9
    ACE estimatorb845.2887.4555.6924.7
    High-quality reads126,402101,97799,098138,537
Relative abundance (%) of bacteria and archaeal phyla
    Euryarchaeota36.210.517.115.2
    Acidobacteria0.1NDND0.1
    Actinobacteria0.10.1ND0.3
    Aegiribacteria0.6ND1.7ND
    Armatimonadetes0.17.60.11.3
    Atribacteria0.50.10.20.2
    Bacteroidetes7.518.622.032.3
    Caldiserica ND0.1NDND
    Calditrichaeota0.2ND0.10.1
    Chloroflexi6.929.85.82.1
    Cloacimonetes0.30.4ND1.0
    Dependentiae0.1NDND1.7
    EdwardsbacteriaNDND0.5ND
    Elusimicrobia0.10.1ND0.1
    Campylobacterota0.90.1NDND
    Firmicutes3.411.81.029.0
    Kiritimatiellaeota0.71.6ND0.1
    LatescibacteriaNDND3.6ND
    Lentisphaerae0.10.2ND0.1
    MondulibacteriaND0.57.8ND
    Nitrospirae0.70.24.22.9
    Planctomycetes0.30.80.61.4
    Proteobacteria28.29.331.94.7
    Spirochaetes4.33.01.92.0
    Synergistetes1.81.2ND1.8
    Verrucomicrobia3.80.3ND0.2
    ZixibacteriaND0.2ND0.3
    Others2.83.31.33.1
  • a ND, not detected.

  • b ACE, abundance-based coverage estimator.