TABLE 1

Summary of geochemical and sequence data by sample sitea

LakeCoordinatespHbAlkalinityb (mg/liter CaCO3)Conductivityb,e (μS/cm)Sample date (mo/yr)Source materialNo. of readsTaxonomic identification (no. of reads [%])cTotal no. of reads identified as chloroplasts (%)d
Border41.79386°N, 102.53521°W9.8–10.39,300–71,40024,500–65,9456/2007Water11,587Proteobacteria (6,500 [56.1]), Cyanobacteria (2,344 [20.2]), Bacteroidetes (793 [6.8])1,725 (14.9)
6/2008Water11,862Cyanobacteria (6,332 [53.4]), Proteobacteria (2,039 [25.6]), unassigned (1,297 [10.9])3,027 (25.5)
10/2008Water12,771Proteobacteria (33,916 [30.7]), Cyanobacteria (3,769 [29.5]), Bacteroidetes (2,233 [17.5])181 (1.4)
Ellsworth42.06078°N, 102.28409°W9.72,29013,21010/2008Water10,946Actinobacteria (7,174 [65.6]), Proteobacteria (2,048 [18.7]), unassigned (650 [5.9])169 (1.5)
Kokjohn41.78245°N, 102.52274°W9.5–9.92,672–27,2006,070–70,0006/2008Water10,651Actinobacteria (4,647 [43.6]), Proteobacteria (3,277 [30.8]), unassigned (1,027 [9.6])31 (0.3)
10/2008Sediment7,334Proteobacteria (2,811 [38.3]), unassigned (1,729 [23.6]), Firmicutes (795 [10.8])30 (0.4)
Merritt42.06846°N, 102.29020°W9.4390–3,2208,33010/2008Water9,803Cyanobacteria (6,630 [67.6]), Proteobacteria (1,721 [17.6]), Actinobacteria (859 [8.8])13 (0.1)
10/2008Sediment4,198Proteobacteria (1,138 [27.1]), unassigned (752 [17.9]), Bacteroidetes (652 [15.5])324 (7.7)
Perrin41.76924°N, 102.51555°W8.6–9.0450–522800–1,0406/2007Water12,099Proteobacteria (5,854 [48.4]), Bacteroidetes (3,391 [28.0]), Actinobacteria (1,725 [14.3])184 (1.5)
Smith41.78609°N, 102.52386°W8.3–8.9470–502148–8906/2007Water11,732Cyanobacteria (3,659 [31.2]), Proteobacteria (3,500 [29.8]), Actinobacteria (2,090 [17.8])115 (1.0)
10/2008Water10,566Cyanobacteria (4,617 [43.7]), Proteobacteria (2,501 [23.7]), unassigned (1,875 [17.7])4,119 (39.0)
Tree Claim41.78248°N, 102.49649°W7.5–9.9501–9,8001,700–17,8666/2008Water9,404Actinobacteria (3,748 [39.9]), Proteobacteria (2,155 [22.9]), Bacteroidetes (1,078 [11.5])79 (0.8)
10/2008Water9,749Cyanobacteria (6,553 [67.2]), Proteobacteria (2,045 [21.0]), unassigned (563 [5.8])80 (0.8)
10/2008Sediment7,016Cyanobacteria (3,573 [50.9]), Proteobacteria (1,506 [21.5]), unassigned (815 [11.6])2,066 (29.4)
Louden42.07929°N, 102.20402°W9.32,8109,4506/2008Water12,297Cyanobacteria (10,525 [85.6]), Proteobacteria (784 [6.4]), unassigned (318 [2.6])104 (0.8)
  • a Geochemical values are reported as a range of observed values, when possible, to account for seasonal variation.

  • b Values from Shaffer et al. (6), Roberts (7), Zlotnik et al. (14), and Shinneman et al. (15).

  • c Read numbers were calculated by multiplying the total number of reads by the percentage of reads assigned to the taxon. Cyanobacteria include chloroplast-identified sequences.

  • d Total number of reads and percentage of total reads identified as chloroplasts.

  • e μS, microSiemens.