Draft Genome Sequence of Tetzosporium hominis VT-49 gen. nov., sp. nov., Isolated from the Dental Decay Plaque of a Patient with Periodontitis

ABSTRACT Here, we report the draft genome sequence of Tetzosporium hominis VT-49 gen. nov., sp. nov., isolated from the dental plaque of a patient with severe periodontal disease. The draft genome sequence was 2,780,751 bp in length with a 43.3% G+C content. We detected 3,001 genes, which are predicted to encode proteins that regulate both virulence and antibiotic resistance.

T he novel Tetzosporium genus was isolated using previously described combined culture-based and gene-based methods (1-3) from the dental plaque of a patient with severe periodontitis. T. hominis VT-49 is a Gram-positive, aerobic, and rod-shaped bacterium.
The 16S rRNA gene of T. hominis VT-49 was found to possess Յ96% similarity with different members of the Planococcaceae family, namely, Planococcus spp., Paenisporosarcina spp., and Sporosarcina spp. The genome of T. hominis VT-49 was sequenced using a HiSeq 2500 platform (GAIIx, Illumina, USA) according to the manufacturer's instructions, yielding 2,780,751 bp (43.4% GϩC content) with a 120-fold coverage. The VT-49 genome sequence was assembled to 393 contigs using SPAdes version 3.5.0 and annotated using the NCBI Prokaryotic Genome Annotation Pipeline (4,5). The VT-49 genome harbored 66 tRNAs, 15 rRNAs, 4 noncoding RNA operons, and 4,048 proteincoding sequences. Although in silico DNA-DNA hybridization (DDH) analysis is required to identify the species within the same order, we performed this analysis to doublecheck the 16S rRNA gene data. The DDH analysis performed using the genome-togenome distance calculator algorithm confirmed that the genomes of T. hominis VT-49 and Planococcus spp., Paenisporosarcina spp., and Sporosarcina spp. belonged to two different species with a DDH value of less than 25%, which was well below the threshold value of 70% (6,7).
In the VT-49 genome, we identified the presence of protein-coding genes that confer resistance to antibiotics, such as the lactam utilization protein LamB, an organic hydroperoxide resistance protein, as well as multidrug-resistant transporters of the ATP-binding cassette (ABC) family, the multidrug and toxic compound extrusion (MATE) family, and the major facilitator superfamily (MFS). Virulence factors, such as hemolysin D, metalloproteases, peptidases, deoxyribonucleases, ribonucleases, flagellar components, and adhesins, were identified in the VT-49 genome. Moreover, we identified the virulence factors that were typical to the collagenase-like protease and amine oxidase of other periodontal pathogens (11). Follow-up studies on T. hominis VT-49 and bacteriophages associated with this microorganism would enable us to understand explicitly its implications in human pathologies and its possible role in periodontal pathologies (12,13).
Accession number(s). This complete genome sequence has been deposited in GenBank under the accession no. NOKQ00000000.

ACKNOWLEDGMENTS
We thank the Applied Bioinformatics Center (BFX) at the NYU School of Medicine for providing bioinformatics support and helping with the analysis and interpretation of the data. This work has used computing resources at the High-Performance Computing Facility (HPCF) of the Center for Health Informatics and Bioinformatics at the NYU Langone Medical Center.