Complete Genome Sequence of Spiroplasma monobiae MQ-1T (ATCC 33825), a Bacterium Isolated from the Vespid Wasp (Monobia quadridens)

ABSTRACT Spiroplasma monobiae MQ-1T (ATCC 33825) was isolated from the hemolymph of an adult vespid wasp (Monobia quadridens) collected in Maryland. Here, we report the complete genome sequence of this bacterium to facilitate the investigation of its biology and the comparative genomics among Spiroplasma species.

S piroplasma monobiae is known to be associated with the vespid wasps (Hymenoptera: Vespidae) in North America (1). The type strain MQ-1 T was isolated from the hemolymph of an adult Monobia quadridens collected in Maryland and is the representative of group VII within the genus (2). Several interesting features were identified in the early characterization of this bacterium, including its possession of the smallest genome within the Apis clade (3,4), its unique pattern of DNA methylation (5), and the potent effect of inducing tumor necrosis factor alpha secretion in mammalian cells by its membrane (6). To facilitate future investigation of the biology of this bacterium, as well as to improve the taxon sampling of available Spiroplasma sequences for comparative genomics and evolutionary studies (7), we determined its complete genome sequence.
The strain was acquired from the American Type Culture Collection (catalog number ATCC 33825). The freeze-dried sample was processed according to the manufacturer's instructions and cultured in M1D medium (8) prior to DNA extraction using the Wizard genomic DNA purification kit (Promega, USA). PCR and Sanger sequencing were performed to verify that the 16S rRNA gene sequence matched the reference record (GenBank accession number GU585673).
The complete genome sequence of Spiroplasma monobiae MQ-1 T consists of one circular chromosome that is 891,575 bp in size with a GϩC content of 27.8%; no plasmid was found. The first version of the annotation includes one set of 16S-23S-5S rRNA genes, 29 tRNAs (covering all 20 amino acids), 813 protein-coding genes, and 2 pseudogenes; no CRISPR locus was found.
Accession number(s). The complete genome sequence of Spiroplasma monobiae MQ-1 T has been deposited at DDBJ/EMBL/GenBank under the accession number CP025543.

ACKNOWLEDGMENTS
The Sanger sequencing service was provided by the Genomic Technology Core Facility of our institute. The Illumina paired-end sequencing service was provided by Yourgene Bioscience (New Taipei City, Taiwan).
The funding for this project was provided by the Institute of Plant and Microbial Biology at Academia Sinica and the Ministry of Science and Technology of Taiwan (NSC 101-2621-B-001-004-MY3 and MOST 104-2311-B-001-019) to C.-H.K. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.