Complete Genome Sequence of Agrobacterium tumefaciens 1D1609

ABSTRACT Agrobacterium tumefaciens 1D1609 is a highly virulent strain isolated from a crown gall tumor of alfalfa (Medicago sativa L.). Compared to other well-characterized A. tumefaciens strains, such as C58 and Ach5, 1D1609 has a distinctive host range. Here, we report its complete genome sequence to facilitate future studies.

A grobacterium tumefaciens is known for its ability to transfer a DNA segment on the tumor-inducing (Ti) plasmid into the nuclear genome of its plant hosts (1). Because of this property, A. tumefaciens is widely used in genetic engineering (2,3). The strain A. tumefaciens 1D1609 was isolated from a crown gall on a field-grown alfalfa plant in Imperial Valley, southern California (4). Previous infection assays demonstrated that this strain has an infectivity profile distinct from those of other well-characterized strains, such as C58 and Ach5 (4,5). Thus, comparative analysis among these A. tumefaciens strains could shed light on the genetic determinants of host range and infection efficiency, which would improve their biotechnological applications. To facilitate such studies, we determined the complete genome sequence of A. tumefaciens 1D1609 and report the results here.

Accession number(s).
The complete genome sequence of A. tumefaciens 1D1609 has been deposited at DDBJ/EMBL/GenBank under the accession numbers CP026924 to CP026928.

ACKNOWLEDGMENTS
We thank the Genomic Technology Core of our institute for providing the Sanger sequencing service. The Illumina paired-end sequencing service was provided by the Genomics Core Facility (Institute of Molecular Biology, Academia Sinica), and the Illumina mate pair sequencing service was provided by Yourgene Bioscience (New Taipei City, Taiwan).
Funding for this project was provided by the Institute of Plant and Microbial Biology at Academia Sinica and the Ministry of Science and Technology of Taiwan (grant MOST 105-2311-B-001-067) to C.-H.K.
The funders had no role in the study design, data collection and interpretation, or the decision to submit the work for publication.