Draft Genome Sequence of Bacillus velezensis Lzh-a42, a Plant Growth-Promoting Rhizobacterium Isolated from Tomato Rhizosphere

ABSTRACT The plant growth-promoting rhizobacterium Bacillus velezensis strain Lzh-a42, which has antimicrobial activity, was isolated from tomato rhizosphere. Here, we report its genome sequence, which includes several predicted functional genes related to secondary metabolite biosynthesis, antimicrobial activity, and biofilm synthesis.

B. velezensis strain Lzh-a42 was isolated from tomato rhizosphere soil samples from the city of Dezhou, Shandong Province, China. This PGPR strain had antimicrobial activity toward some plant pathogens, including Fusarium moniliforme.
From this study, we report the draft genome sequence of B. velezensis Lzh-a42. Whole-genome DNA was extracted and then sequenced using the PacBio and Illumina MiSeq systems, respectively. The raw data were filtered and assembled with SPAdes version 3.9.0 (8) and A5-miseq version 20150522 (9), which generated 1,237 Mb of total clean data with a genome coverage of 278.0ϫ. Two scaffolds were finally obtained, and the total length of the genome was 4,246,605 bp, with a GC content of 45.99%, which is similar to those of B. velezensis subsp. plantarum YAU B9601-Y2 (99%) (GenBank accession no. HE774679), B. velezensis Y2 (99%) (CP003332), and B. velezensis CN026 (99%) (CP024897).
The genome sequence of B. velezensis Lzh-a42 presented here will help us to understand the strain's mechanisms for antimicrobial activity and biofilm synthesis and its potential use as a biocontrol agent for disease management and enhancing agricultural productivity.
Accession number(s). This whole-genome shotgun project has been deposited in GenBank under the accession no. CP025308.

ACKNOWLEDGMENTS
This work was supported by the Natural Science Foundation of Shandong Province (ZR2015CQ002), the Taishan Young Scholars Program of Shandong Province of China (tsqn20161049), the Talent Introduction Project of Dezhou University of China (2016kjrc10), the Projects of Science and Technology for Colleges and Universities in Shandong Province (J17KA099 and J15LE09), and the National Natural Science Foundation of China (31500606 and 61671107).