Draft Genome Sequence of Byssochlamys sp. Isolate BYSS01, a Filamentous Fungus Adapted to the Fuel Environment

ABSTRACT Byssochlamys sp. isolate BYSS01 (anamorph, Paecilomyces sp.), which was isolated from jet fuel, is highly adapted to grow in hydrocarbons, having predicted genes involved in degradation of n-alkanes, branched alkanes, and aromatic compounds. The draft genome size is estimated at 29 Mb, containing 8,509 proteins.

T he filamentous fungus Byssochlamys sp. isolate BYSS01 was recovered from Jet A fuel and identified based on morphological characteristics and sequence similarity (94%) to the 18S rRNA gene of Byssochlamys fulva. Byssochlamys sp. (teleomorph) belongs to the genus Paecilomyces, which comprises formaldehyde-resistant and heatresistant food spoilage fungi (1)(2)(3)(4). It was confirmed that the BYSS01 isolate metabolizes alkanes and aromatic hydrocarbons efficiently; this is the first report of a Byssochlamys species adapted to kerosene fuel. Therefore, to gain a deeper understanding of the adaptive mechanisms in isolate BYSS01, its genome was sequenced.
The Kyoto Encyclopedia of Genes and Genomes (KEGG) database and BLASTP searches identified important proteins involved in carbon and energy metabolism (n ϭ 49). In agreement with the ability of BYSS01 to grow in fuel, genes involved in the degradation of aromatics and n-alkanes, including cytochrome P450 alkane hydroxylase, cytochrome P450 monooxygenase, aromatic ring-opening dioxygenase, salicylate hydroxylase, 2-haloacid dehalogenase, benzyl alcohol dehydrogenase, benzoate 4-monooxygenase, and dimethylsulfide monooxygenase, were found. This genome sequence will help to understand the adaptive mechanisms employed by fungi to survive and proliferate in hydrocarbonbased fuels.
Accession number(s). This whole-genome shotgun project has been deposited at DDBJ/ENA/GenBank under the accession number NIXA00000000.

ACKNOWLEDGMENT
This material is based on research sponsored by AFRL/RQTF under agreement number FA8650-16-2-2605.
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