Complete Genome Sequence of Spiroplasma corruscae EC-1T (DSM 19793), a Bacterium Isolated from a Lampyrid Beetle (Ellychnia corrusca)

ABSTRACT Spiroplasma corruscae EC-1T (DSM 19793) was isolated from the gut of a lampryid beetle (Ellychnia corrusca) collected in Beltsville, MD, USA, in 1983. Here, we report the complete genome sequence of this bacterium to facilitate the investigation of its biology and the comparative genomics among Spiroplasma species.

S piroplasma corruscae is known to be associated with firefly beetles (Coleoptera: Lampyridae) and tabanid flies (Diptera: Tabanidae) in North America (1). EC-1 T was isolated from the gut of a lampryid beetle (Ellychnia corrusca) collected in Beltsville, MD, USA, in 1983 and was designated the representative of group XIV within the genus. To facilitate future investigation on the biology of this bacterium, as well as to improve the taxon sampling of available Spiroplasma sequences for comparative genomics and evolutionary studies (2), we determined the complete genome sequence of S. corruscae EC-1 T .
The strain was acquired from the German Collection of Microorganisms and Cell Cultures (catalog number DSM 19793). The freeze-dried sample was processed according to the manufacturer's instruction and cultured in the M1D medium (3) prior to DNA extraction using the Wizard Genomic DNA purification kit (Promega, USA). PCR and Sanger sequencing were performed to verify that the 16S rRNA gene sequence matched the reference record (GenBank accession number AY189128) (4).
Accession number(s). The complete genome sequence of Spiroplasma corruscae EC-1 T has been deposited at DDBJ/EMBL/GenBank under the accession numbers CP022535 (chromosome) and CP022536 (plasmid).

ACKNOWLEDGMENTS
The bacterial strain was imported under permit number 103-B-001 issued by the Council of Agriculture of Taiwan. The Sanger sequencing service and the Illumina sequencing library preparation service were provided by the Genomic Technology Core (Institute of Plant and Microbial Biology, Academia Sinica). The Illumina MiSeq sequencing service was provided by the DNA Sequencing Core (Institute of Molecular Biology, Academia Sinica).
The funding for this project was provided by the Institute of Plant and Microbial Biology at Academia Sinica and the Ministry of Science and Technology of Taiwan (NSC 101-2621-B-001-004-MY3 and MOST 104-2311-B-001-019) to C.-H.K. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.