Metagenome-Assembled Genome Sequences of Acetobacterium sp. Strain MES1 and Desulfovibrio sp. Strain MES5 from a Cathode-Associated Acetogenic Microbial Community

ABSTRACT Draft genome sequences of Acetobacterium sp. strain MES1 and Desulfovibrio sp. strain MES5 were obtained from the metagenome of a cathode-associated community enriched within a microbial electrosynthesis system (MES). The draft genome sequences provide insight into the functional potential of these microorganisms within an MES and a foundation for future comparative analyses.

M icrobial communities inhabiting the cathode of microbial electrosynthesis systems (MES) have been examined for their potential to utilize cathode-derived electrons for CO 2 conversion into acetate (1,2). Due to the abundance of Acetobacterium spp. (30%) and Desulfovibrio spp. (20%) on the cathode surface and their potential importance within the MES, we have determined two draft genome sequences from the cathode metagenome to (i) elucidate the metabolic pathways each genome encodes and (ii) ascertain the role each may play in electrode-dependent CO 2 fixation.
Metagenome sequencing, assembly, and genome binning were performed as described previously (3,4). Briefly, a dual-sequencing approach of Illumina MiSeq pairedend sequencing (V2 chemistry, 2 ϫ 250 bp) and Pacific Biosciences sequencing (P4-C2 chemistry) was utilized. MiSeq reads were quality trimmed using the CLC Genomics Workbench and used for PacBio read error correction. The corrected PacBio reads were assembled with Velvet (version 1.2) (5), and Acetobacterium-specific contigs were used as the template to map and bin Acetobacterium-specific MiSeq reads. Mapped reads were assembled with SPAdes (version 3.5.0) (6), and Velvet-assembled PacBio contigs were merged into the SPAdes assembly as trusted contigs for graph construction, gap closure, and repeat resolution. To obtain the Desulfovibrio sp. strain MES5 draft genome sequence, DNA was extracted from anaerobic MES enrichment cultures growing on 1,2-propanediol. Illumina paired-end reads (56% Desulfovibrio) were assembled with metaSPAdes (default k-mer values), and contigs were binned with VizBin (7). Both genomes were manually curated for the removal of duplicate single-copy marker genes based upon CheckM (version 1.0.7) output.
Both genomes were annotated with Rapid Annotations using Subsystems Technology (RAST) version 2.0 using the RASTtk pipeline (11,12) and the NCBI Prokaryotic Genome Annotation Pipeline (http://www.ncbi.nlm.nih.gov/genome/annotation_prok/), and further comparative genomic exploration of each genome is under way.
Accession number(s). The Acetobacterium sp. strain MES1 and Desulfovibrio sp. strain MES5 draft genome sequences have been deposited in the GenBank database under the accession numbers MJUY00000000 and MJUZ00000000, respectively.

ACKNOWLEDGMENT
Funding was provided by the U.S. Department of Energy, Advanced Research Project Agency-Energy (award DE-AR0000089).