Draft Genome Sequence of Pseudomonas pachastrellae Strain CCUG 46540T, a Deep-Sea Bacterium

ABSTRACT Pseudomonas pachastrellae strain CCUG 46540T (KMM 330T) was isolated from a deep-sea sponge specimen collected in the Philippine Sea at a depth of 750 m. The draft genome has an estimated size of 4.0 Mb, exhibits a G+C content of 61.2 mol%, and is predicted to encode 3,592 proteins, including pathways for the degradation of aromatic compounds.

P seudomonas pachastrellae strain CCUG 46540 T (KMM30 T ) was isolated from a deepsea sponge (Pachastrella sp.) in 1991 and in 2005 was proposed as the type strain of this species (1). It is affiliated with the P. pertucinogena phylogenetic group, as established by Mulet and collaborators (2). P. pachastrellae is a strictly aerobic mesophilic species that tolerates up to 10% NaCl.
The whole-genome shotgun sequence of P. pachastrellae was performed using pair-end sequencing on the MiSeq platform system (Illumina). The Newbler Assembler version 2.7 software package (Roche) was used for de novo genome assembly. The draft genome size is 3,920,464 bp and contains 119 contigs, with an average contig length of 32,945 kb, a median coverage depth of 160ϫ, and an average GϩC of 61.2 mol%.
Prediction and annotation of the genome was performed using the NCBI Prokaryotic Genome Annotation pipeline (http://www.ncbi.nlm.nih.gov/genome/annotation_prok). Analysis and comparison of the functional annotation was done using the KEGG Automatic Annotation Server (KAAS) (3).
The genome is one of the smallest in the Pseudomonas genus. A total of 3,656 coding sequences, 54 tRNA sequences, and one rRNA sequence were identified in the chromosome. Flagellation and twitching motility genes have been found. Secretion systems of types I, II, and VI were localized in the genome, together with Sec-independent protein translocases. A complete set of genes was detected for the upper and lower degradation pathways predicted to catabolize benzene and toluene. A multicomponent phenol hydroxylase produces catechol that is metabolized by a lower metacleavage pathway to central metabolites. Genes were predicted for the assimilation of nitrate and nitrite as nitrogen sources (nitrate, nitrite, and ammonium transporter systems, as well as nitrate and nitrite reductases with their regulators), for a urea transport system, and for urease synthesis (although the strain was described as urease negative). Genes for catalase/peroxidase, for an iron complex transport system, and for an NADPHdependent ferric siderophore reductase were also predicted. A complete set of genes was found for the synthesis of polyhydroxyalkanoate as a storage compound. CRISPR-associated proteins and a putative toxin/antitoxin system were annotated. No plasmids were detected, but the mobilome was characterized by 41 proteins annotated as transposases, some of them related to the IS5, ISL3, Tn3, and Tn5 families. Moreover, 1,150 encoded proteins were annotated as hypothetical proteins without a predicted function.
The three closest species to P. pachastrellae in the P. pertucinogena phylogenetic group (P. aestusnigri, P. oceani, and P. sabulinigri) have a marine origin-some from the deep sea, such as P. oceani (4). P. pachastrellae strains have also been found in polluted samples after a marine oil spill (5), like strains of P. aestusnigri (6), the phylogenetically closest species in the group. The sequenced genome of P. pachastrellae CCUG 46540 T will help to elucidate the phylogeny of the species in this group and also unravel the metabolic mechanisms shared by these microorganisms for survival in these environments.
Accession number(s). This whole-genome shotgun project has been deposited in DDBJ/ENA/GenBank under the accession number MUBC00000000. The version described in this paper is the first version, MUBC01000000.

ACKNOWLEDGMENTS
This work was supported by project CGL2015-70925-P from the Spanish MINECO, with Fondo Europeo de Desarrollo Regional (FEDER) cofunding. Margarita Gomila was supported by a postdoctoral contract from the Conselleria d'Innovació, Recerca I Turisme del Govern de les Illes Balears and the European Social Fund.