Complete Genome Sequence of Klebsiella oxytoca Strain JKo3

Klebsiella oxytoca can be either pathogenic or beneficial, depending on conditions. These opposing characteristics have not been fully elucidated. Here, we report the complete sequence of the K. oxytoca JKo3 genome, consisting of a single circular chromosome of 5,943,791 bp and four plasmids.

and contains nonmotile rod-shaped Gram-negative bacteria. Klebsiella oxytoca is a free-living bacterium that can be isolated from various plants and animals, including humans. The species can be either pathogenic or beneficial depending on conditions, because it occasionally causes colitis in humans but also promotes the growth of plants and insects as an endophytic diazotroph (1)(2)(3)(4)(5)(6)(7)(8)(9). These bilateral characteristics of the species require further elucidation. To provide a genetic basis for such analyses, we determined the genome sequence of strain JKo3, which is a clinical isolate in the strain collection at the Jikei University School of Medicine in Japan (10).
The sequence of JKo3 was determined using the 454 GS FLX Titanium system. Using the GS Assembler software version 2.6, a total of 466,772 single-end reads (162 Mb in total) and 141,520 8-kb paired-end reads (44 Mb) were assembled into eight scaffolds containing 92 gaps. Finishing was first performed by in silico analysis using GenoFinisher (11), and then remaining gaps were closed by sequencing gap-spanning PCR products using an ABI3130xl DNA sequencer (Applied Biosystems). To correct sequence errors, a paired-end library of JKo3 was constructed using the TruSeq DNA sample prep kit (Illumina) and sequenced by Illumina MiSeq (2 ϫ 150 bp). Mapping of the obtained 1,998,167 reads to the JKo3 genome sequence and single-nucleotide polymorphism (SNP) calling were performed using BWA (12) and SAMtools (13), respectively. A total of 20 insertions/deletions were corrected by the resequencing. Gene identification and annotation were conducted by the Microbial Genome Annotation Pipeline (MiGAP [http://www.migap.org]).
The chromosome of JKo3 is 5,943,791 bp in size. It contains eight copies of rRNA operons, 83 tRNA genes, and 5,647 protein-coding genes. K. oxytoca is known to contain a nif gene cluster, which is a model system for studying nitrogen fixation (6). The nif gene cluster of JKo3 consisted of 20 genes and was found in a 23-kb chromosome region. The number and orientation of genes were consistent with a previous report (10,14). In addition, JKo3 has one small plasmid (plasmid 1 [3,897 bp], con-taining four protein-coding genes), and three large plasmids (plasmid 2 [80,479 bp], plasmid 3 [104,259 bp], and plasmid 4 [208,080 bp], containing 50, 91, and 101 protein-coding genes, respectively). These plasmids could be used for the construction of a set of plasmid vectors useful in the molecular or biotechnology fields because they are compatible with each other. The complete genome sequence of K. oxytoca JKo3 presented here could provide useful information for investigating the beneficial and nosocomial pathogenic characteristics of K. oxytoca.

ACKNOWLEDGMENTS
We thank all persons involved in the study.
A part of the study was supported by a grant-in-aid for Young Scientists (A) (grant no. 24687032), a grant-in-aid for Young Scientists (B) (grant no. 22770241), a grant-in-aid for challenging Exploratory Research (grant no. 16K14821), and a grant-in-aid for Scientific Research on Innovative Areas, "Genome science," from the Ministry of Education, Culture, Sports, Science and Technology of Japan (grant no. 221S0002).

FUNDING INFORMATION
This work, including the efforts of Tadayuki Iwase, was funded by Ministry of Education, Culture, Sports, Science and Technology (MEXT) (24687032, 22770241, and 16K14821). This work, including the efforts of Tetsuya Hayashi, was funded by Ministry of Education, Culture, Sports, Science and Technology (MEXT) (221S0002).