Complete Genome Sequences of Pseudomonas aeruginosa Phages vB_PaeP_PcyII-10_P3P1 and vB_PaeM_PcyII-10_PII10A

vB_PaeP_PcyII-10_P3P1 and vB_PaeM_PcyII-10_PII10A are Pseudomonas aeruginosa bacteriophages belonging, respectively, to the Lit1virus genus of the Podoviridae family and the Pbunavirus genus of the Myoviridae family. Their genomes are 72,778 bp and 65,712 bp long, containing 94 and 93 predicted open reading frames, respectively.

the host strain, two virulent bacteriophages were isolated in Orsay, France, in the year 2014. Phage vB_PaeP_PcyII-10_P3P1 (P3P1) was found in compost and produced large clear plaques, whereas phage vB_PaeM_PcyII-10_PII10A (PII10A) was isolated from wastewater and formed small clear plaques. PII10A and P3P1 could be propagated on nine and 10, respectively, out of 27 tested clinical strains representative of the P. aeruginosa diversity (1). They were not capable of forming plaques on three different mutants of the lipopolysaccharide (LPS) biosynthesis pathway (2). The morphology of phage virions was determined using transmission electron microscopy (TEM). TEM was performed at 80 kV in JEM-100B (JEOL) with use of negative contrast with 1% uranyl acetate. P3P1 showed a 74 Ϯ 0.6-nm icosahedral capsid with a short tail, characteristic of Podoviridae, and PII10A showed a 70 Ϯ 1-nm head and a 130 Ϯ 3-nm nonflexible contractile tail characteristic of Myoviridae.
Phage DNA was sequenced in an Illumina MiSeq 300-bp paired-end run with a 900-bp insert library produced by mechanical shearing at the IMAGIF Sequencing facility. Qualitycontrolled trimmed reads were assembled, using Geneious R9, to a single linear contig at mean coverages of 2,932-fold for P3P1 and 2,447-fold for PII10A. The P3P1 genome was 72,778 bp long, with a GϩC content of 56%. A 671-bp direct terminal region was observed, and a homopolymer of Cs was found at position 71026 in an intergenic region upstream of open reading frame (ORF)86. Heterogeneity in the number of Cs reduced the sequencing quality at this position. The PII10A genome was 65,712 bp long with a GC content of 55.5%. Automatic ORF detection and annotation were performed with RAST, and the obtained annotations were confronted to function predictions made using Phagonaute, a website allowing the display of distant protein homologies detected with HHsearch (3), within a genomic context (4). Analysis with Phagonaute (probability threshold of 90%, followed by manual inspection) had contrasting effects on the two genomes. For PII10A, the number of annotated ORFs increased from 13 to 36: 12 ORFs were correctly annotated, one ORF was moved back to "hypothetical protein," and 24 ORFs more received predictive annotations. In contrast, for phage P3P1, the number of annotated ORFs decreased from 38 to 32, which we interpret as RAST overannotations: 25 annotations were conserved, nine ORFs were moved back to "hypothetical protein," and four ORFs received a different annotation. Only three additional genes (terminase small subunit, HNH endonuclease, and tail fiber protein) were annotated.
The overall P3P1 gene organization was that of phages vB_PaeP_C2-10_Ab09 and Lit1, with the long RNA polymerase characteristic of N4-like viruses (5). PII10A showed a gene organization similar to that of PB1-like phages and was most closely related to vB_PaeM_PAO1_Ab27 (1,6). The highest level of differences was seen in the tail length tape-measure protein (ORF15), which was highly similar to that of phage NH-4 (7) and phages KTN6 and KT28 (8).
Accession number(s). The complete sequences of P. aeruginosa phages P3P1 and PII10A have been deposited in the European Nucleotide Archive (ENA) under the accession numbers LT594787 and LT594786, respectively, within BioProject PRJEB14592, together with the raw sequence reads. The versions described in this paper are the second versions.

ACKNOWLEDGMENTS
The present work has benefited from the facilities and expertise of the Electron Microscopy facilities of Imagerie-Gif (http://www.i2bc.paris -saclay.fr/spip.php?article282). This core facility is a member of the Infrastructures en Biologie Santé et Agronomie (IBiSA) and is supported by the French National Research Agency under Investments for the Future programs "France-BioImaging," and the Labex "Saclay Plant Science" (ANR-10-INSB-04-01 and ANR-11-IDEX-0003-02, respectively).
The research was funded by grant "Resisphage" ANR-13-ASTRID-0011-01 from Direction Générale de l'Armement (DGA) and the Agence Nationale de la Recherche (ANR, France). The funders had no role in the study design, data collection and interpretation, or the decision to submit the work for publication. crossmark Genome Announcements November/December 2016 Volume 4 Issue 6 e00916-16 genomea.asm.org 1