Draft Genome Sequences of Two Novel Pseudomonas Strains Exhibiting Differential Hypersensitivity Reactions on Tobacco and Corn Seedlings

Two novel Pseudomonas strains (S1E40 and S3E12) isolated from corn roots are antagonistic to Rhizoctonia solani and exhibit differential hypersensitivity reactions on tobacco and corn seedlings. We report here the draft genome sequences of strains S1E40 and S3E12, consisting of 6.98 and 7.06 Mb with 6,150 and 6,129 predicted protein-coding sequences, respectively.

drenched with a 10% soil suspension were characterized by sequencing the 16S rRNA and three housekeeping genes (gyrB, rpoB, and rpoD), Biolog carbon utilization, and hypersensitivity reactions on tobacco and corn seedlings. Strains S1E40 and S3E12 are identified as potential novel species within the genus Pseudomonas. Both strains are antagonistic (in vitro) to a fungal plant pathogen, Rhizoctonia solani. However, these novel strains exhibit differential hypersensitivity reactions (HRs) on tobacco and corn seedlings. Strain S3E12 induces HRs on tobacco and corn seedlings, but strain S1E40 does not. We report here the draft genome sequences of Pseudomonas strains S1E40 and S3E12. The draft genome sequences were determined by paired-end sequencing using Illumina MiSeq technology (Génome Québec, Montreal, Canada). A total of 4,290,298 and 4,472,574 paired-end reads, each 250 bp in length, for strain S1E40 and S3E12, respectively, were generated. The quality of the reads was checked using FastQC version 0.11.3 (1). De novo assemblies were performed using ABySS version 1.5.2 (2) at k-mer values of 75 to 113. k-mer values of 85 and 95 gave the best assembly, producing 49 and 38 scaffolds after the discard of scaffolds with length Ͻ300 bp for strains S1E40 and S3E12, respectively. The total size of the draft genome is 6,984,066 bp (minimum, 402 bp; maximum, 834,588 bp; N 50 , 206,686 bp) and 7,062,659 bp (minimum, 303 bp; maximum, 935,416 bp; N 50 , 420,043 bp) for S1E40 and S3E12, respectively. The GϩC content of the draft genomes is 61.6% and 60.9%, with overall estimated coverage of 153ϫ and 158ϫ for strains S1E40 and S3E12, respectively.
Accession number(s). This whole-genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession numbers MAUE00000000 (strain S1E40) and MBDT00000000 (strain S3E12). The versions described in this paper are the first versions, MAUE01000000 and MBDT01000000, respectively.

ACKNOWLEDGMENTS
This project study is funded by Agriculture and Agri-Food Canada through projects 1136 (Mycology and Bacteria Biosystematics: Filling the gaps) and J-000409 (Monitoring an emerging bacterial pathogen of corn using next generation sequencing).
We are grateful to the staff of Génome Québec, McGill University, Montreal, Canada, for technical assistance.

FUNDING INFORMATION
This work, including the efforts of Caetanie F. Tchagang, was funded by Gouvernement du Canada | Agriculture and Agri-Food Canada (AAFC) (1136 and J-000409).
We are grateful for the financial support.