Genome Sequence of the Acetogenic Bacterium Moorella mulderi DSM 14980T

Here, we report the draft genome sequence of Moorella mulderi DSM 14980T, a thermophilic acetogenic bacterium, which is able to grow autotrophically on H2 plus CO2 using the Wood-Ljungdahl pathway. The genome consists of a circular chromosome (2.99 Mb).

is gaining more interest as a valuable tool for the generation of renewable energy and value-added chemicals (1-3). Thus, homoacetogenic bacteria that use the Wood-Ljungdahl pathway for the CO 2 fixation process have proven to be a main component in this research field (3-8). Among the numerous species of homoacetogens, three organisms have been relatively well studied (Moorella thermoacetica, Acetobacterium woodii, and Clostridium ljungdahlii) (9)(10)(11)(12)(13). However, several relevant species remain poorly studied, and the genetic information of many of them remains almost nonexistent or is very limited. Therefore, in this study, we report the draft genome sequence of Moorella mulderi DSM 14980 T a thermophilic homoacetogenic anaerobic bacterium originally isolated from a bioreactor with methanol as the energy source (14). Similar to M. thermoacetica, M. mulderi DSM 14980 T is able to grow on several substrates, including methanol, H 2 -CO 2 , pyruvate, and glucose. However, several differences have been reported. The optimal temperature of M. mulderi DSM 14980 T (65°C) is higher than the optimal temperature reported for M. thermoacetica (55 to 60°C). Moreover, in contrast to M. thermoacetica, M. mulderi DSM 14980 T is able to grow on lactate but cannot use nitrate as an electron acceptor (14).
The MasterPure complete DNA purification kit (Epicentre, Madison, WI, USA) was used to isolate the chromosomal DNA of M. mulderi DSM 14980 T . Isolated DNA was used to generate Illumina shotgun sequencing libraries. Sequencing was performed by employing a MiSeq system using MiSeq reagent kit version 3 (600 cycles), as recommended by the manufacturer (Illumina, San Diego, CA, USA), resulting in 2,785,408 paired-end reads (300 bp) that were trimmed using Trimmomatic 0.32 (15). De novo assembly performed with the SPAdes genome assembler software version 3.6.2 (16) resulted in 72 contigs (Ͼ500 bp) and an average coverage of 188.5-fold.
The genome of M. mulderi DSM 14980 T probably consists of a circular chromosome of (2.99 Mb) with an overall GϩC content of 53.32%. Gene prediction and annotation were performed using Rapid Prokaryotic Genome Annotation (Prokka) (17). The genome harbored 3 rRNA genes, 52 tRNA genes, 2,240 protein-coding genes with predicted functions, and 859 genes coding for hypothetical proteins. The cluster of genes encoding enzymes of the methyl and carbonyl branches of the Wood-Ljungdahl pathway is conserved within acetogenic bacteria (18). Therefore, M. mulderi DSM 14980 T shows an arrangement identical to the pattern previously identified in M. thermoacetica strains ATCC 39073 and DSM 521 T (10,18). The cluster is composed of eight genes (acsFABCV, cooC, and acsDE) encoding the subunits of the CO dehydrogenase-acetyl-coenzyme A (CoA) synthase complex. The genes encoding the two subunits of the methylene-THF reductase (metVF) are located four genes downstream of this cluster.
Nucleotide sequence accession numbers. This whole-genome shotgun project has been deposited at DDBJ/ENA/GenBank under the accession no. LTBC00000000. The version described in this paper is version LTBC01000000.

ACKNOWLEDGMENTS
We thank the DAAD for funding and Kathleen Gollnow for technical support.
The funder (DAAD) had no role in the study design, data collection and interpretation, or the decision to submit the work for publication.

FUNDING INFORMATION
This work, including the efforts of Genis Andrés Castillo Villamizar, was funded by German Academic Exchange Service Bonn Office (DAAD Bonn).