Draft Genome Sequence of Psychrobacter piscatorii Strain LQ58, a Psychrotolerant Bacterium Isolated from a Deep-Sea Hydrothermal Vent

Here, we report the 3.1-Mb draft genome sequence of Psychrobacter piscatorii strain LQ58, isolated from a deep-sea hydrothermal vent on the East Pacific Rise. The sequence will provide further insight into the environmental adaptation of psychrotolerant bacteria and the development of novel cold-active enzymes for industrial application.

psychrophilic bacteria, which are widely distributed in marine and terrestrial environments (1)(2)(3). They have been considered good sources of cold-active enzymes for industrial and environmental uses (4)(5)(6). Psychrobacter piscatorii strain LQ58 (ϭMCCC 1A10701) is an aerobic, psychrotolerant, chemoorganotrophic bacterium, isolated from a deep-sea hydrothermal vent sediment sample collected on the East Pacific Rise at a depth of 2,906 m (3.10 S, 102.55 W). Growth was observed at temperatures between 0°C and 35°C with an optimum at 22°C in the presence of 2% NaCl. Strain LQ58 showed highest 16S rDNA sequence similarity with P. piscatorii T-3-2 (99.3%) (7), followed by Psychrobacter aquaticus CMS56 (98.6%) (8). P. piscatorii was reported to exhibit an extraordinarily high catalase activity (9). To date, there is no reference P. piscatorii genome sequence available publicly. Here, we report the draft genome sequence of strain LQ58, the first released P. piscatorii genome sequence, which will provide further insight into the environmental adaptation of psychrotolerant bacteria and the development of novel cold-active enzymes for industrial application.
Extraction of genomic DNA from strain LQ58 was carried out using a bacterial DNA kit (Omega) according to the manufacturer's instructions. The genome of strain LQ58 was sequenced by wholegenome shotgun sequencing using the Illumina MiSeq system at Shanghai Majorbio Bio-pharm Technology Co., Ltd. (Shanghai, China). A total of 804,651,728 reads were generated, representing an approximately 260-fold coverage of the genome. These reads were assembled using SOAPdenovo version 2.0 (10) and resulted in 139 contigs with an N 50 of 47,940 bp. The size of the longest contig was 149,861 bp. The draft genome of strain LQ58 was analyzed and annotated using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) (http://www.ncbi.nlm.nih.gov/genome/annotation_prok). Genes of interest were manually evaluated.
The draft genome sequence of strain LQ58 was 3,089,314 bp in length, with a GϩC content of 44.1%. The genome contains 2,676 predicted open reading frames and 2,562 predicted protein-coding sequences). There were 33 tRNA genes, 3 rRNA genes, and 1 noncoding RNA (ncRNA) gene predicted from this assembly.
Nucleotide sequence accession numbers. This whole-genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession number LNDJ00000000. The version described in this paper is the first version, LNDJ01000000.

ACKNOWLEDGMENTS
This work was supported by grants from the National Basic Research Program of China (973 Program, no. 2012CB722209), the Natural Science Foundation of Fujian Province (no. 2015J01136). The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

FUNDING INFORMATION
This work, including the efforts of Meixian Zhou, was funded by National Basic Research Program of China (2012CB722209). This work, including the efforts of Meixian Zhou, was funded by Natural Science Foundation of Fujian Province (Fujian Provincial Natural Science Foundation) (2015J01136).