Complete Genome Sequences of Two Interactive Moderate Thermophiles, Paenibacillus napthalenovorans 32O-Y and Paenibacillus sp. 32O-W

Microorganisms with the capability to desulfurize petroleum are in high demand with escalating restrictions currently placed on fuel purity. Thermophilic desulfurizers are particularly valuable in high-temperature industrial applications. We report the whole-genome sequences of Paenibacillus napthalenovorans 32O-Y and Paenibacillus sp. 32O-W, which can and cannot, respectively, metabolize dibenzothiophene.

ing restrictions on fuel purity, and alkylated derivatives of dibenzothiophene (DBT) are among the most common contaminants in petroleum fuel precursors (1). Thermophilic microorganisms capable of desulfurization are especially valuable, as they can be used in high temperature industrial settings (2)(3)(4)(5). Previous research isolated two bacterial strains, Paenibacillus napthalenovorans 32O-Y, which can metabolize DBT, and Paenibacillus sp. 32O-W, which cannot. Curiously, when the two strains are in a mixed culture, 32O-Y metabolizes DBT at a higher rate than in isolation (6). Here, we present the whole-genome sequences of both 32O-Y and 32O-W.
Assembly of 32O-W (5,200,139 bp, 56.34% GϩC, 93ϫ coverage) was completed using the HGAP2 protocol in SMRT-Analysis version 2.2.0 (7). The assembly was trimmed and then corrected via sequential runs of the RS_Resequencing.1 protocol. Basecalling was further polished by mapping Illumina reads (169ϫ coverage) to the assembly using Geneious version 7.1.9 (8).
HGAP2 assembly of 32O-Y produced six contigs. Illumina reads were assembled de novo using SPAdes version 3.5.0 (9) and Geneious and compared to the HGAP2 assembly. 32O-Y HGAP2 contigs were joined using overlaps from the other assemblies. This assembly (5,375,858 bp, 49.69% GϩC; PacBio 109ϫ, Illumina 122ϫ) was then trimmed and corrected as above.
Nucleotide sequence accession numbers. Whole-genome sequences were deposited in GenBank under the accession numbers CP013652 (32O-Y) and CP013653 (32O-W). Both species are in the ARS Culture Collection, Peoria, IL, USA, under NRRL catalog numbers B-65351 and B-65352. sequencing; and Yukun Sun and Shadi Beidas (IIT) for help with software and scriptwriting.

FUNDING INFORMATION
This work was supported by start-up funds from the Illinois Institute of Technology to Jean-François Pombert.