Complete Genome Sequence of Streptomyces sp. Strain CCM_MD2014, Isolated from Topsoil in Woods Hole, Massachusetts

Here, we present the complete genome sequence of Streptomyces sp. strain CCM_MD2014 (phylum Actinobacteria), isolated from surface soil in Woods Hole, MA. Its single linear chromosome of 8,274,043 bp in length has a 72.13% G+C content and contains 6,948 coding sequences.

the members of which are Gram-positive bacteria that are ubiquitous in soils and typically have a genome with a high GϩC content. They are known for their capacity for secondary metabolite synthesis and expression of novel enzymes (1). The strain in this study, Streptomyces sp. CCM_MD2014, was cocultured with Curtobacterium sp. strain MR_MD2014 from topsoil around a rusty fire hydrant in Woods Hole, MA (41°31=44.65ЉN 70°40=21.5ЉW) on 7 July 2014, using isolation protocols modified from those of El-Nakeeb and Lechevalier (2). This organism was cultivated as part of the 2014 Microbial Diversity Summer Program at the Marine Biological Laboratory in Woods Hole, MA.
DNA was extracted from the coculture using the Promega Wizard genomic DNA purification kit with 1 h of lysozyme digestion. The DNA was quantified using the Promega QuantiFluor double-stranded DNA (dsDNA) system and then size selected for a minimum length of 4 kb. Size-selected DNA was sequenced on a Pacific Biosciences RSII sequencing platform with P5C3 chemistry. The sequenced fragments were assembled using HGAP3 on the SMRT Portal (3). The final assembled genome consisted of a single linear chromosome that was 8,274,043 bp long, with a 72.13% GϩC content and sequencing coverage of 89ϫ.
Nucleotide sequence accession number. The complete genome sequence of Streptomyces sp. strain CCM_MD2014 is available through GenBank under the accession number CP009754. Promega Corporation donated the molecular reagents used in this project. The sequencing for this organism was supported and carried out by Pacific Biosciences. We thank George O'Toole, Alison Butler, Emil Ruff, Arpita Bose, Suzanne Kern, Louis Kerr, Kurt Dahlstrom, Alice Michel, and Cristian Salgado for their assistance and advice.

ACKNOWLEDGMENTS
R.J.R. works for New England BioLabs, a company that sells research reagents, including restriction enzymes and DNA methylases, to the scientific community. J.K. and M.B. are full-time employees at Pacific Biosciences, a company that develops single-molecule real-time sequencing technologies, including the sequencing platform used in this project.
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