Complete Genome Sequence of Citrobacter freundii Myophage Michonne

Citrobacter freundii is a Gram-negative opportunistic pathogen that causes dangerous infections such as neonatal meningitis. C. freundii also harbors antibiotic resistance, making phages infecting this host valuable tools. Here, we announce the complete genome of the C. freundii FelixO1-like myophage Michonne and describe its notable features.

testinal tracts of animals and humans. It acts as an opportunistic pathogen, causing urinary tract and blood infections, often nosocomial in nature, and is a major cause of neonatal meningitis (1). C. freundii also exhibits high antimicrobial resistance to penicillins and third-generation cephalosporins (2), emphasizing the need for alternative treatments like bacteriophages. The novel FelixO1-like myophage Michonne, described here, may be useful in the biocontrol of C. freundii.
Bacteriophage Michonne was isolated from a sewage sample collected in College Station, TX. Phage DNA was sequenced in an Illumina MiSeq 250-bp paired-end run with a 550-bp insert library at the Genomic Sequencing and Analysis Facility at the University of Texas (Austin, TX, USA). Quality controlled, trimmed reads were assembled to a single contig of circular terminally redundant assembly at 56.3-fold coverage using SPAdes version 3.5.0 (3). The contig was confirmed to be complete by PCR using primers that faced the upstream and downstream ends of the contig. Products from the PCR amplification of the junctions of concatemeric molecules were sequenced by Sanger sequencing (Eton Bioscience, San Diego, CA). Genes were predicted using GeneMarkS (4) and corrected using software tools available on the Center for Phage Technology (CPT) Galaxy instance (https://cpt .tamu.edu/galaxy-public/). Morphology was determined using transmission electron microscopy performed at the Texas A&M University Microscopy and Imaging Center.
The genome of Michonne has FelixO1-like genes, including those whose products are involved in morphogenesis, biosynthesis, DNA replication, and lysis. The two phages differ mostly in hypothetical proteins of unknown function. The tape measure protein of Michonne is preceded by a tail assembly protein that uses a translational frameshift to the Ϫ1 reading frame to achieve a second product, as is seen in many Caudovirales (13). Lysis genes identified include a putative class-III holin, a soluble lysozyme, and inner and outer spanin genes (14,15).
Nucleotide sequence accession number. The genome sequence of phage Michonne was contributed to GenBank with the accession number KT001916.

ACKNOWLEDGMENTS
This work was supported primarily by funding from award number EF-0949351, "Whole Phage Genomics: A Student-Based Approach," from the National Science Foundation. Additional support came from the Center for Phage Technology, an Initial University Multidisciplinary Research Initiative supported by Texas A&M University and Texas AgriLife, and from the Department of Biochemistry and Biophysics.
We are grateful for the advice and support of the CPT staff. This announcement was prepared in partial fulfillment of the requirements for BICH464 Phage Genomics, an undergraduate course at Texas A&M University.