Draft Genome Sequence of Mycobacterium lentiflavum CSUR P1491

We announce the draft genome sequence of Mycobacterium lentiflavum strain CSUR P1491, a nontuberculous mycobacterium responsible for opportunistic potentially life-threatening infections in immunocompromised patients. The genome described here comprises a 6,818,507-bp chromosome exhibiting a 65.75% G+C content, 6,354 protein-coding genes, and 75 RNA genes.

T he initial description of Mycobacterium lentiflavum was based on three isolates belonging to a single DNA homology group (1). Further phylogenetic analyses found Ͼ99.5% similarity in the 16S rRNA gene sequence with Mycobacterium simiae and Mycobacterium genavense (1). This observation qualified M. lentiflavum as a member of the Mycobacterium simiae complex. This environmental organism (2) has been isolated from human specimens, including an intervertebral disc (1), respiratory tract samples and gastric/gut aspirates (1, 3), lymph nodes (4), feces (5), and bone marrow (5). M. lentiflavum emerges in cystic fibrosis patients (6) and was responsible for the death of one heart-transplanted immunocompromised patient (5). Routine identification of this fastidious mycobacterium can be achieved by partial rpoB gene sequencing (7).
We sequenced the whole genome of M. lentiflavum to exact the phylogenetic relationships within the M. simiae complex, design tools for its genotyping, and disclose any genotypic pattern possibly related to host-mycobacterium relationships.
Genomic DNA was extracted from M. lentiflavum strain CSUR P1491 (isolated from the sputum of a cystic fibrosis patient) grown on Middlebrook 7H10 solid medium at 37°C in a 5% CO 2 atmosphere for 3 weeks. Genomic DNA was sequenced on Illumina MiSeq throughout three runs, including one paired-end Nextera and two mate pair libraries, in a 2 ϫ 250-bp run for each barcoded library. The whole set of reads was trimmed using Trimmomatic (8), then assembled through the assembler software SPAdes v3.5 (9,10). Contigs obtained were combined together by SSPACE v2 (11), Opera v2 (12) helped by GapFiller v1.10 (13), and homemade tools to refine the set.
In silico DNA-DNA hybridization (DDH) was performed with the genome-to-genome distances between pairs of two closed genomes (25). The genome of M. lentiflavum was locally aligned 2-by-2 using the BLAT algorithm (26,27) against M. simiae and M. genavense. The DDH was estimated from a generalized linear model using a specific distance formula (28), resulting in following values: 27.5% (Ϯ2.43) with M. simiae and 34.6% (Ϯ2.47) with M. genavense. These data confirm M. lentiflavum as a unique species clearly distinct from M. simiae and M. genavense.
Nucleotide sequence accession numbers. The M. lentiflavum strain CSUR P1491 genome sequence has been deposited at EMBL under the accession numbers CTEE01000001 to CTEE01000005.

ACKNOWLEDGMENT
This study was supported by URMITE, IHU Méditerranée Infection, Marseille, France.