Complete Genome Sequence of the Attenuated Novobiocin-Resistant Streptococcus iniae Vaccine Strain ISNO

Streptococcus iniae ISNO is an attenuated novobiocin-resistant vaccine strain. Its full genome is 2,070,182 bp in length. The availability of this genome will allow comparative genomics to identify potential virulence genes important for pathogenesis of S. iniae and potential mechanisms associated with novobiocin resistance in this strain.

pathogen that causes infections in both humans and fish (1)(2)(3). As a serious marine and freshwater fish pathogen, S. iniae causes significant economic losses to aquaculture (4,5). This pathogen was originally isolated from Amazon freshwater dolphin (Inia geoffrensis) in 1976 (6). However, S. iniae causes diseases not only to dolphin, but also to many fish species, including rainbow trout (Oncorhynchus mykiss [7]), barramundi (Lates calcarifer [8]), red drum (Sciaenops ocellatus [9]), flounder (Paralichthys spp. [10,11]), and tilapia (Oreochromis spp. (12)]. A highly virulent strain, S. iniae ISET0901, was cultured from diseased Nile tilapia (Oreochromis niloticus) during a disease outbreak in 2005 (13). Through selection for resistance of S. iniae ISET0901 to novobiocin, an attenuated vaccine strain, S. iniae ISNO, was obtained (13). The vaccine strain S. iniae ISNO was found to be totally safe to Nile tilapia at various exposure doses and in back passage safety studies (13). In addition, the vaccine strain S. iniae ISNO offered Nile tilapia significant protection against S. iniae infections at a wide range of efficacious immunization doses (13). Furthermore, the vaccine strain S. iniae ISNO provided tilapia significant protection up to 6 months after a single vaccination (13). Compared to the virulent parent S. iniae ISET0901, the vaccine strain S. iniae ISNO was~1,000-fold resistant to novobiocin. However, what changes occurred at the genomic DNA level were unknown. Therefore, the complete genome sequence of S. iniae ISNO was determined in this study.
The genome of S. iniae ISNO was sequenced using the Illumina 1500 HiSeq platform. BioNumerics (Applied Maths) was used to assemble a total of 15,690,266 sequence reads with an average length of 92.32 bp (estimated 700ϫ coverage) using the complete genome of S. iniae SF1 (GenBank accession no. CP005941) as reference. The assembled genome of S. iniae ISNO is 2,070,182 bp, with GϩC content of 36.8%. RNAmmer (14) predicted 12 copies of ribosomal RNA (4 copies of 5S RNA, 16S RNA, and 23S RNA, respectively), similar to reference genome S. iniae SF1 (15). The RAST server (16) predicted 1,980 coding sequences belonging to 303 subsystems, including 291 involved in carbohydrate catabo-lism, 149 in protein metabolism, 135 in synthesis of amino acids and derivatives, 114 in cell wall and capsule synthesis, 95 in RNA metabolism, and 92 in DNA metabolism (including 77 in cofactors, vitamins, prosthetic groups, or pigments; 63 in nucleoside and nucleotide synthesis; 62 in fatty acid and lipid synthesis; 52 in virulence, disease, and defense; 47 in membrane transport; 37 in stress response; 31 in phosphorus metabolism; 26 in regulation and cell signaling; 7 in secondary metabolism; and 2 in motility and chemotaxis).
Nucleotide sequence accession number. The complete genome sequence of S. iniae ISNO was deposited at GenBank under the accession no. CP007587.
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We thank James Becnel (USDA-ARS) and Laura Silo-Suh (Mercer University) for critical reviews of the manuscript. We thank Beth Peterman (USDA-ARS) for her technical support.