Complete Genome Sequence of an F8-Like Lytic Myovirus (φSPM-1) That Infects Metallo-β-Lactamase-Producing Pseudomonas aeruginosa

Pseudomonas aeruginosa is an important cause of infection, especially in immunocompromised patients. In this regard, strains producing carbapenemases, mainly metallo-β-lactamases (MBLs), have become a significant public health concern. Here, we present the complete annotated genome sequence (65.7 kb) of an F8-related lytic myovirus (Pbunalikevirus genus) that infects MBL-producing P. aeruginosa strains.

nosa strains have become a significant public health problem (1). Here, we report the sequencing and annotation of a lytic myovirus infecting MBL-producing P. aeruginosa.
A lytic myovirus, called SPM-1, was recovered from a collection of environmental isolates (from surface water samples from rivers in southeastern Brazil) and oldest-typing-set phages (2); it was propagated in an environmental SPM-1-producing P. aeruginosa host strain (3) and then was characterized by transmission electron microscopy and complete-genome sequencing. This phage was able to lyse clonally unrelated MBLproducing P. aeruginosa strains (4), including the Brazilian endemic clone harboring the bla SPM-1 MBL gene, sequence type 277 (ST277) (5). The phage had an elongated icosahedral head with an approximately 50-nm diameter and a tail near 70 nm in length, showing that it belongs to the Myoviridae family of the order Caudovirales (6).
The bacteriophage genome was sequenced by Ion Torrent PGM (Life Technologies) using the Ion Xpress Plus fragment library kit, with size selection by electrophoresis before emulsion PCR on an Ion OneTouch apparatus, using the Ion PGM sequencing 400 and Ion PGM template OT2 400 kits. A total of 82,540 reads (~200ϫ coverage), with a mean length of 159 bp, were obtained after sequencing, and de novo assembly of next-generation sequencing (NGS) reads (CLC bio Genomics Workbench version 6.5) yielded 19 contigs (7).
The whole-genome sequence (WGS) of the myophage was assembled using crossmatch alignment (40-bp minimum overlap), and the prediction of protein-coding sequences (CDSs) was obtained using phage annotation tools and methods (PhAnToMe) (http://www.phantome.org). Manual curation of opening reading frames (ORFs) was carried out by comparative genomics using BLASTp (http://www.ncbi.nlm.nih.gov/blast/Blast.cgi) analysis of proteins against the GenBank NR database.
The WGS of SPM-1 comprises 65,729 bp, with a GϩC content of 54.97%, 92 ORFs, and no tRNAs, harboring genetic information needed for replication of nucleic acid and synthesis of protein coats (i.e., genes coding for DNA replication, capsid, tail, packaging, and lytic enzymes). Moreover, the SPM-1 genome is 95.6% identical to that of the P. aeruginosa F8 phage (GenBank accession no. NC_007810.1). In this regard, a 237-bp region, which is absent in SPM-1 and present in the F8 phage, partially encodes a hypothetical protein of unknown function in the F8 phage genome. The start point of the F8 genome sequence was positioned at a locus that possesses a CDS that is predicted to code for a tail-length tape-measure protein (locus tag PPGF8SP_0065). We have therefore marked the genome sequence as circular. In this respect, the linear supercontig was extended by adding 100 ambiguous "N" bases on either side. Alignment of the reads to this supercontig was then carried out using CLC Genomics Workbench, and their extension was possible in regions that had a read coverage of Ͼ10ϫ and that spanned the supercontig ends and the ambiguous "N" bases added; this yielded a 40-bp overlap of the extension, circularizing the sequence.
Finally, the SPM-1 phage genome has high identities to other P. aeruginosa phages belonging to the Pbunalikevirus genus (8 Nucleotide sequence accession number. The complete genome sequence of F8-like lytic myovirus SPM-1 (Pbunalikevirus genus) was deposited in GenBank with the accession no. KF981875.