Genome Sequences of Two Klebsiella pneumoniae Isolates from Different Geographical Regions, Argentina (Strain JHCK1) and the United States (Strain VA360)

We report the sequences of two Klebsiella pneumoniae clinical isolates, strains JHCK1 and VA360, from a newborn with meningitis in Buenos Aires, Argentina, and from a tertiary care medical center in Cleveland, OH, respectively. Both isolates contain one chromosome and at least five plasmids; isolate VA360 contains the Klebsiella pneumoniae carbapenemase (KPC) gene.

acquired infections (1-7) and its virulence seems to be enhanced by its ability to acquire resistance to antibiotics, including carbapenems (3). Only a few virulence factors were identified, including a pathogenicity island in some strains (8)(9)(10). After nucleotide and restriction-map comparisons among several strains, a high heterogeneity zone was proposed (11)(12)(13).
De novo short-read assembly was also performed for each genome using IDBA-UD (16), resulting in 228 (97.17% of the reads) and 210 (96.54%) contigs Ͼ500 bp for JHCK1 and VA360, respec-tively. The draft genomes of JHCK1 and VA360 consist of 6,016,101-bp and 5,578,970-bp sequences, respectively, with an average GϩC content of 57.5% for both. These two genome sequences were annotated using an in-house implementation of the Ergatis annotation pipeline (17).
There are 5,812 and 5,414 predicted protein coding genes within the genomes of K. pneumoniae isolates JHCK1 and VA360. Of these, 20% and 18% of the protein coding genes are annotated as hypothetical or conserved hypothetical proteins. Of those with functional predictions, 40 and 22 are associated with phages/ prophages, while 129 and 103 are associated with resistance to antibiotics or toxin production. A bla KPC gene is likely located within the pKpQI-IT-like plasmid in strain VA360. JHCK1 and VA360 genomes have 80 and 83 tRNA genes, respectively, as well as 4 rRNA genes.
Of all the predicted genes, 4,973 are common between the JHCK1 and VA360 genomes, although some of the other genes do have homologs in other sequenced genomes. Of the remaining 839 genes in JHCK1, 267 have an assigned function, including involvement in iron acquisition, conjugative transfer, and copper, arsenic, fosfomycin, mercury, and chromate resistance. Similarly, of the 551 VA360 genes not found in JHCK1, 175 have an assigned function, including those involved in monosaccharide metabolism, citrate metabolism/transport, and resistance to aminoglycosides (adenylyltransferase). These functions may be indicative of strain-specific capabilities.
Nucleotide sequence accession numbers. The GenBank accession numbers for K. pneumoniae JHCK1 (Argentina) and VA360 (Cleveland, OH) are ANGH00000000 and ANGI00000000, respectively. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.