Draft Genome Sequence of Streptomyces bottropensis ATCC 25435, a Bottromycin-Producing Actinomycete

A series of bottromycin antibiotics have been isolated and identified from Streptomyces bottropensis strain ATCC 25435. Here, a draft genome sequence of S. bottropensis ATCC 25435 is presented. The genome carries an intact biosynthetic gene cluster for bottromycin antibiotics, which provides insight into the combinatorial biosynthesis of bottromycin antibiotics.

All the reads up to 1,199 Mb were obtained, which represents a 134-fold coverage of the genome. Finally, we obtained the draft genome (GϩC content, 71.53%) of S. bottropensis ATCC 25435, with a size of 8,914,727 bp distributed in 43 scaffolds that include 109 contigs.
The genome consists of one linear chromosome with 4 rRNA operons, 70 tRNA genes, and 8,253 protein-coding genes (CDSs); among the CDSs, 2,882 proteins could be assigned to COG families, 3,814 proteins could be assigned to KEGG orthology, and 734 proteins have no match to any proteins in the NR databases.
The putative gene cluster for the biosynthesis of bottromycins, localized on the chromosome (scaffold 9), has a highly similar organization to the gene clusters described from Streptomyces sp. WMMB272 (13) and Streptomyces sp. BC 16019 (14). It contains all of the open reading frames (ORFs) reported in Streptomyces sp. BC 16019 and Streptomyces sp. WMMB272, including 3 different radical SAM-dependent enzyme genes, 1 precursor peptide gene, 2 cyclode hydratase (YcaO-like family) genes, 1 putative ␣/␤ hydrolase gene, 1 putative amidohydrolase gene, 1 cytochrome P450 enzyme gene, 1 transcriptional regulator gene, 1 O-methyltransferase gene, 1 multidrug transporter gene, and 1 leucyl-aminopeptidase gene. All of the putative proteins show extremely high similarities (most of them 99%) to their counterparts in Streptomyces sp. BC 16019 and Streptomyces sp. WMMB272, which suggests that they might share the same biosynthetic mechanisms.
Thus, mining the S. bottropensis ATCC 25435 genome will further elucidate the chemical and genetic diversity of this strain for the discovery of novel gene clusters and bioactive compounds. The information provided by the genome sequence is of great importance for guiding further development of bottromycin derivatives.
The genomic sequence of S. bottropensis ATCC 25435 not only provides a basis for the exploration of this strain for biotechnology applications but also is valuable for systematic studies of related strains.
Nucleotide sequence accession numbers. This Whole Genome Shotgun project has been deposited at DDBJ/EMBL/ GenBank under the accession no. AOCF00000000. The version described in this paper is the first version, AOCF01000000.