Draft Genome Sequence of the Symbiotically Competent Cyanobacterium Nostoc sp. Strain KVJ20

Nostoc sp. KVJ20 at different life stages. (A) Diazotrophically grown Nostoc sp. KVJ20 at exponential stage, with vegetative cells arranged in multicellular filaments interspersed by nitrogen-fixing cells and heterocysts (HC). (B) Motile filaments and hormogonia. (C and D) A filament differentiating resting cells and akinetes (C) and mature resting cells and akinetes (D). (E) Phylogenetic tree highlighting the position of Nostoc sp. KVJ20 (in bold) relative to other strains within the Nostocaceae family. The genomes of strain names marked with an asterisk (*) have been sequenced. The strains and their corresponding GenBank accession numbers for 16S rRNA genes are Nostoc sp. KVJ20, LSSA00000000; Nostoc punctiforme PCC73102, AF027655; Nostoc calcicola III, AJ630447; Nostoc edaphicum X, AJ630449; Desmonostoc sp. strain PCC6302, HG004582; Cylindrospermum stagnale PCC7417, AJ133163; Nostoc sp. strain PCC7120, BA000019; Aphanizomenon flos-aquae 1tu26s2, AJ630443; and Nodularia spumigena PCC73104, DQ185241. 16S rRNA genes, aligned using MUSCLE (10, 11), were used as input to the Web server Phylogeny.fr (12). A total of 1,402 positions, not including gaps, were compared and used to reconstruct and analyze the phylogenetic relationships. Confidence index values are shown in red, and the bar represents the amount of nucleotide substitutions per site. Gloeobacter violaceus (GenBank accession number FR798924) was used as an outgroup.