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Genome Sequences

Complete Genome Sequence of Agrobacterium sp. Strain 33MFTa1.1, Isolated from Thlaspi arvense Roots

Sasha Langley, Thomas Eng, Kenneth H. Wan, Robin A. Herbert, Andrew P. Klein, Yasuo Yoshikuni, Susannah G. Tringe, James B. Brown, Susan E. Celniker, Jenny C. Mortimer, Aindrila Mukhopadhyay
Catherine Putonti, Editor
Sasha Langley
aBioSciences Area, Lawrence Berkeley National Laboratory, Berkeley, California, USA
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Thomas Eng
aBioSciences Area, Lawrence Berkeley National Laboratory, Berkeley, California, USA
bJoint BioEnergy Institute, Emeryville, California, USA
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  • ORCID record for Thomas Eng
Kenneth H. Wan
aBioSciences Area, Lawrence Berkeley National Laboratory, Berkeley, California, USA
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Robin A. Herbert
aBioSciences Area, Lawrence Berkeley National Laboratory, Berkeley, California, USA
bJoint BioEnergy Institute, Emeryville, California, USA
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  • ORCID record for Robin A. Herbert
Andrew P. Klein
cDepartment of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
dHoward Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
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Yasuo Yoshikuni
aBioSciences Area, Lawrence Berkeley National Laboratory, Berkeley, California, USA
eU.S. DOE Joint Genome Institute, Walnut Creek, California, USA
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Susannah G. Tringe
aBioSciences Area, Lawrence Berkeley National Laboratory, Berkeley, California, USA
eU.S. DOE Joint Genome Institute, Walnut Creek, California, USA
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James B. Brown
aBioSciences Area, Lawrence Berkeley National Laboratory, Berkeley, California, USA
fCentre for Computational Biology, Biosciences, University of Birmingham, Birmingham, United Kingdom
gDepartment of Statistics, University of California, Berkeley, California, USA
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Susan E. Celniker
aBioSciences Area, Lawrence Berkeley National Laboratory, Berkeley, California, USA
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Jenny C. Mortimer
aBioSciences Area, Lawrence Berkeley National Laboratory, Berkeley, California, USA
bJoint BioEnergy Institute, Emeryville, California, USA
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Aindrila Mukhopadhyay
aBioSciences Area, Lawrence Berkeley National Laboratory, Berkeley, California, USA
bJoint BioEnergy Institute, Emeryville, California, USA
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Catherine Putonti
Loyola University Chicago
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DOI: 10.1128/MRA.00432-19
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ABSTRACT

Agrobacterium sp. strain 33MFTa1.1 was isolated for functional host-microbe interaction studies from the Thlaspi arvense root-associated microbiome. The complete genome is comprised of a circular chromosome of 2,771,937 bp, a linear chromosome of 2,068,443 bp, and a plasmid of 496,948 bp, with G+C contents of 59%, 59%, and 58%, respectively.

ANNOUNCEMENT

Agrobacterium is a diverse genus of soil-dwelling bacteria in the alphaproteobacterial family Rhizobiaceae. Many Agrobacterium species cause plant diseases, including Agrobacterium tumefaciens (crown gall disease), Agrobacterium rhizogenes (hairy root disease), and Agrobacterium vitis (lesions and tumors on grape vines). First described in 1897 (1), Agrobacterium has been widely studied, largely because of its ability to transform plant cells with its DNA (which is known as transfer DNA [T-DNA]). As a result, A. tumefaciens has become the workhorse of plant genetic engineering (2–4). Other strains of Agrobacterium are commensal inhabitants of plant tissue. For example, Agrobacterium sp. strain 33MFTa1.1 was isolated from the root endophytic compartment of Thlaspi arvense, a close relative of the model plant Arabidopsis thaliana (5), and recolonizes gnotobiotic A. thaliana plants without producing disease symptoms (6, 7). This report describes the complete genome sequence of Agrobacterium sp. 33MFTa1.1 and will facilitate plant-microbe interaction studies.

Agrobacterium sp. 33MFTa1.1 (NCBI taxon identifier [ID] 1279031) was obtained from Jeff Dangl. A previously published (5) draft shotgun assembly of this strain consists of 15 contigs, and we posited that long-read sequencing techniques would enable assembly at the chromosome level. Bacteria were streaked onto LB plates, single colonies were amplified, and an aliquot was used for 16S V1 and V4 PCR (8) and sequence identification (reviewed in reference 9). DNA was isolated (10), and whole-genome sequencing was performed at Lawrence Berkeley National Laboratory (LBNL) using a combination of Oxford Nanopore long-read sequencing on the MinION Mk1B (11) and Illumina paired-end 300-bp read sequencing for quality (12). Oxford Nanopore sequencing libraries were constructed from 5 to 10 μg DNA using the Oxford Nanopore 1D native barcoding genomic DNA protocol (version NBE_9006_v103_revO_21Dec2016) and sequenced on three FLO-MIN107 R9 version flow cells. Oxford Nanopore data were demultiplexed with Porechop (13). Sequencing yielded 61,147 reads with a length of ≥2,000 bp and a filtered mean read length of 6,862 bp, totaling 419,565,480 bp (∼79-fold coverage). The Illumina sequencing library was constructed from 1.5 μg DNA. The DNA was fragmented using a Diagenode Bioruptor, and libraries were constructed using the NEBNext Ultra DNA library prep kit for Illumina. Sequencing yielded 2,529,890 paired-end reads, which were trimmed using Trimmomatic (14), resulting in a filtered mean read length of 270 bp and totaling 631,387,669 bp (∼128-fold coverage). Nanopore and Illumina sequencing data were used as inputs for a de novo hybrid assembly constructed using Unicycler version 0.4.1 with the “bold” option (15, 16). The assembly produced 3 contigs, a single circular chromosome, a single linear chromosome, and a plasmid. Annotations of protein-encoding open reading frames and noncoding RNAs (ncRNAs) were predicted with the NCBI Prokaryotic Genome Annotation Pipeline (17).

The circular chromosome annotation predicts 2,654 protein-coding genes, 63 pseudogenes, 2 rRNA operons, and 40 tRNAs, with canonical anticodon triplets that base pair with codons for amino acids. It also encodes the telomerase A (telA) gene that encodes the protein required to generate the covalently closed hairpin loops at the ends of linear chromosomes (3). The linear chromosome annotation predicts 1,800 protein-coding genes, 69 pseudogenes, 2 rRNA operons, and 14 tRNAs. In addition, the genome includes a single 496,948-bp plasmid, p_JBx_073812, which contains candidate genes for plasmid replication initiation proteins (repA, repB, and repC) and for conjugative transfer (traA, traB, traC, traD, traF, traG, traH, and traM). It also carries genes for arsenic resistance (arsH and ACR3) and arsenate metabolism (two copies of the arsenate reductase gene arsC). A comparison of the new assembly with the previous 15-contig assembly (NCBI taxon ID 1279031) using the Joint Genome Institute (JGI) microbial species identifier (MiSI) genome-wide average nucleotide identity, alignment fraction (gANI, AF) calculator (https://ani.jgi-psf.org/html/calc.php) reveals high similarity, as expected, with gANI values of 100 and AF values of 0.99 (previous assembly→new assembly) and 0.98 (new assembly→previous assembly) (18).

To identify gene clusters of interest for further research, we analyzed the genome with the antibiotics and Secondary Metabolite Analysis SHell (antiSMASH) version 4.2.0 (19) tool. A total of 41 clusters and putative clusters were identified. These included a type I polyketide synthase cluster, a terpene cluster, and a nonribosomal peptide synthetase cluster. Of the remainder, 5 were putative fatty acid clusters, 5 were putative saccharide clusters, and 28 were putative clusters of unknown type, as identified by the ClusterFinder algorithm (19).

Data availability.The complete circular and linear chromosomes and plasmid sequences described here are deposited in GenBank under the accession numbers CP036358 (circular chromosome), CP036359 (linear chromosome), and CP036360 (plasmid). The SRA accession number is PRJNA523206.

ACKNOWLEDGMENTS

We thank B. Booth for data management. The strain was originally isolated by the lab of Jeffery Dangl at UNC Chapel Hill.

This work was supported by the Laboratory Directed Research and Development Program of Lawrence Berkeley National Laboratory under U.S. Department of Energy contract DE-AC02-05CH11231. S.G.T. and Y.Y. are supported through the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, supported by the Office of Science of the U.S. Department of Energy under contract number DE-AC02-05CH11231. Part of this work was funded through the DOE Joint BioEnergy Institute (http://www.jbei.org), supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, through contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the U.S. Department of Energy.

FOOTNOTES

    • Received 11 June 2019.
    • Accepted 16 August 2019.
    • Published 12 September 2019.
  • Copyright © 2019 Langley et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

REFERENCES

  1. 1.↵
    1. Kado CI
    . 2014. Historical account on gaining insights on the mechanism of crown gall tumorigenesis induced by Agrobacterium tumefaciens. Front Microbiol 5:340. doi:10.3389/fmicb.2014.00340.
    OpenUrlCrossRefPubMed
  2. 2.↵
    1. Nester EW
    . 2014. Agrobacterium: nature’s genetic engineer. Front Plant Sci 5:730. doi:10.3389/fpls.2014.00730.
    OpenUrlCrossRefPubMed
  3. 3.↵
    1. Goodner B,
    2. Hinkle G,
    3. Gattung S,
    4. Miller N,
    5. Blanchard M,
    6. Qurollo B,
    7. Goldman BS,
    8. Cao Y,
    9. Askenazi M,
    10. Halling C,
    11. Mullin L,
    12. Houmiel K,
    13. Gordon J,
    14. Vaudin M,
    15. Iartchouk O,
    16. Epp A,
    17. Liu F,
    18. Wollam C,
    19. Allinger M,
    20. Doughty D,
    21. Scott C,
    22. Lappas C,
    23. Markelz B,
    24. Flanagan C,
    25. Crowell C,
    26. Gurson J,
    27. Lomo C,
    28. Sear C,
    29. Strub G,
    30. Cielo C,
    31. Slater S
    . 2001. Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58. Science 294:2323–2328. doi:10.1126/science.1066803.
    OpenUrlAbstract/FREE Full Text
  4. 4.↵
    1. Wood DW,
    2. Setubal JC,
    3. Kaul R,
    4. Monks DE,
    5. Kitajima JP,
    6. Okura VK,
    7. Zhou Y,
    8. Chen L,
    9. Wood GE,
    10. Almeida NF,
    11. Woo L,
    12. Chen Y,
    13. Paulsen IT,
    14. Eisen JA,
    15. Karp PD,
    16. Bovee D,
    17. Chapman P,
    18. Clendenning J,
    19. Deatherage G,
    20. Gillet W,
    21. Grant C,
    22. Kutyavin T,
    23. Levy R,
    24. Li MJ,
    25. McClelland E,
    26. Palmieri A,
    27. Raymond C,
    28. Rouse G,
    29. Saenphimmachak C,
    30. Wu Z,
    31. Romero P,
    32. Gordon D,
    33. Zhang S,
    34. Yoo H,
    35. Tao Y,
    36. Biddle P,
    37. Jung M,
    38. Krespan W,
    39. Perry M,
    40. Gordon-Kamm B,
    41. Liao L,
    42. Kim S,
    43. Hendrick C,
    44. Zhao ZY,
    45. Dolan M,
    46. Chumley F,
    47. Tingey SV,
    48. Tomb JF,
    49. Gordon MP,
    50. Olson MV,
    51. Nester EW
    . 2001. The genome of the natural genetic engineer Agrobacterium tumefaciens C58. Science 294:2317–2323. doi:10.1126/science.1066804.
    OpenUrlAbstract/FREE Full Text
  5. 5.↵
    1. Levy A,
    2. Salas Gonzalez I,
    3. Mittelviefhaus M,
    4. Clingenpeel S,
    5. Herrera Paredes S,
    6. Miao J,
    7. Wang K,
    8. Devescovi G,
    9. Stillman K,
    10. Monteiro F,
    11. Rangel Alvarez B,
    12. Lundberg DS,
    13. Lu T-Y,
    14. Lebeis S,
    15. Jin Z,
    16. McDonald M,
    17. Klein AP,
    18. Feltcher ME,
    19. Rio TG,
    20. Grant SR,
    21. Doty SL,
    22. Ley RE,
    23. Zhao B,
    24. Venturi V,
    25. Pelletier DA,
    26. Vorholt JA,
    27. Tringe SG,
    28. Woyke T,
    29. Dangl JL
    . 2018. Genomic features of bacterial adaptation to plants. Nat Genet 50:138–150. doi:10.1038/s41588-017-0012-9.
    OpenUrlCrossRef
  6. 6.↵
    1. Lebeis SL,
    2. Paredes SH,
    3. Lundberg DS,
    4. Breakfield N,
    5. Gehring J,
    6. McDonald M,
    7. Malfatti S,
    8. Glavina del Rio T,
    9. Jones CD,
    10. Tringe SG,
    11. Dangl JL
    . 2015. Salicylic acid modulates colonization of the root microbiome by specific bacterial taxa. Science 349:860–864. doi:10.1126/science.aaa8764.
    OpenUrlAbstract/FREE Full Text
  7. 7.↵
    1. Castrillo G,
    2. Teixeira P,
    3. Paredes SH,
    4. Law TF,
    5. de Lorenzo L,
    6. Feltcher ME,
    7. Finkel OM,
    8. Breakfield NW,
    9. Mieczkowski P,
    10. Jones CD,
    11. Paz-Ares J,
    12. Dangl JL
    . 2017. Root microbiota drive direct integration of phosphate stress and immunity. Nature 543:513–518. doi:10.1038/nature21417.
    OpenUrlCrossRef
  8. 8.↵
    1. Yu Z,
    2. Morrison M
    . 2004. Comparisons of different hypervariable regions of rrs genes for use in fingerprinting of microbial communities by PCR-denaturing gradient gel electrophoresis. Appl Environ Microbiol 70:4800–4806. doi:10.1128/AEM.70.8.4800-4806.2004.
    OpenUrlAbstract/FREE Full Text
  9. 9.↵
    1. Slatko BE,
    2. Kieleczawa J,
    3. Ju J,
    4. Gardner AF,
    5. Hendrickson CL,
    6. Ausubel FM
    . 2011. “First generation” automated DNA sequencing technology. Curr Protoc Mol Biol Chapter 7:Unit7.2. doi:10.1002/0471142727.mb0702s96.
    OpenUrlCrossRef
  10. 10.↵
    1. Wilson K
    . 2001. Preparation of genomic DNA from bacteria. Curr Protoc Mol Biol Chapter 2:Unit 2.4. doi:10.1002/0471142727.mb0204s56.
    OpenUrlCrossRef
  11. 11.↵
    1. Jain M,
    2. Olsen HE,
    3. Paten B,
    4. Akeson M
    . 2016. The Oxford Nanopore MinION: delivery of nanopore sequencing to the genomics community. Genome Biol 17:239. doi:10.1186/s13059-016-1103-0.
    OpenUrlCrossRefPubMed
  12. 12.↵
    1. Risse J,
    2. Thomson M,
    3. Patrick S,
    4. Blakely G,
    5. Koutsovoulos G,
    6. Blaxter M,
    7. Watson M
    . 2015. A single chromosome assembly of Bacteroides fragilis strain BE1 from Illumina and MinION nanopore sequencing data. Gigascience 4:60. doi:10.1186/s13742-015-0101-6.
    OpenUrlCrossRef
  13. 13.↵
    1. Wick R
    . 2017. Porechop: adapter trimmer for Oxford Nanopore reads. https://github.com/rrwick/Porechop.
  14. 14.↵
    1. Bolger AM,
    2. Lohse M,
    3. Usadel B
    . 2014. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30:2114–2120. doi:10.1093/bioinformatics/btu170.
    OpenUrlCrossRefPubMedWeb of Science
  15. 15.↵
    1. Chin C-S,
    2. Alexander DH,
    3. Marks P,
    4. Klammer AA,
    5. Drake J,
    6. Heiner C,
    7. Clum A,
    8. Copeland A,
    9. Huddleston J,
    10. Eichler EE,
    11. Turner SW,
    12. Korlach J
    . 2013. Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nat Methods 10:563–569. doi:10.1038/nmeth.2474.
    OpenUrlCrossRefPubMedWeb of Science
  16. 16.↵
    1. Wick RR,
    2. Judd LM,
    3. Gorrie CL,
    4. Holt KE
    . 2017. Unicycler: resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput Biol 13:e1005595. doi:10.1371/journal.pcbi.1005595.
    OpenUrlCrossRefPubMed
  17. 17.↵
    1. Tatusova T,
    2. DiCuccio M,
    3. Badretdin A,
    4. Chetvernin V,
    5. Nawrocki EP,
    6. Zaslavsky L,
    7. Lomsadze A,
    8. Pruitt KD,
    9. Borodovsky M,
    10. Ostell J
    . 2016. NCBI Prokaryotic Genome Annotation Pipeline. Nucleic Acids Res 44:6614–6624. doi:10.1093/nar/gkw569.
    OpenUrlCrossRefPubMed
  18. 18.↵
    1. Varghese NJ,
    2. Mukherjee S,
    3. Ivanova N,
    4. Konstantinidis KT,
    5. Mavrommatis K,
    6. Kyrpides NC,
    7. Pati A
    . 2015. Microbial species delineation using whole genome sequences. Nucleic Acids Res 43:6761–6771. doi:10.1093/nar/gkv657.
    OpenUrlCrossRefPubMed
  19. 19.↵
    1. Weber T,
    2. Blin K,
    3. Duddela S,
    4. Krug D,
    5. Kim HU,
    6. Bruccoleri R,
    7. Lee SY,
    8. Fischbach MA,
    9. Müller R,
    10. Wohlleben W,
    11. Breitling R,
    12. Takano E,
    13. Medema MH
    . 2015. antiSMASH 3.0—a comprehensive resource for the genome mining of biosynthetic gene clusters. Nucleic Acids Res 43:W237–W243. doi:10.1093/nar/gkv437.
    OpenUrlCrossRefPubMed
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Complete Genome Sequence of Agrobacterium sp. Strain 33MFTa1.1, Isolated from Thlaspi arvense Roots
Sasha Langley, Thomas Eng, Kenneth H. Wan, Robin A. Herbert, Andrew P. Klein, Yasuo Yoshikuni, Susannah G. Tringe, James B. Brown, Susan E. Celniker, Jenny C. Mortimer, Aindrila Mukhopadhyay
Microbiology Resource Announcements Sep 2019, 8 (37) e00432-19; DOI: 10.1128/MRA.00432-19

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Complete Genome Sequence of Agrobacterium sp. Strain 33MFTa1.1, Isolated from Thlaspi arvense Roots
Sasha Langley, Thomas Eng, Kenneth H. Wan, Robin A. Herbert, Andrew P. Klein, Yasuo Yoshikuni, Susannah G. Tringe, James B. Brown, Susan E. Celniker, Jenny C. Mortimer, Aindrila Mukhopadhyay
Microbiology Resource Announcements Sep 2019, 8 (37) e00432-19; DOI: 10.1128/MRA.00432-19
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