ABSTRACT
The larvae of the greater wax moth, Galleria mellonella, are pests of active beehives. In infection biology, these larvae are playing a more and more attractive role as an invertebrate host model. Here, we report on the first genome sequence of Galleria mellonella.
GENOME ANNOUNCEMENT
A ubiquitous pest of beehives, the greater wax moth causes severe damage due to its destructive way of feeding (1). As an invertebrate host model, it is used to study the virulence of different pathogens, such as methicillin-resistant Staphylococcus aureus (MRSA) (2), Listeria monocytogenes (3), and Candida spp. (4). So far, molecular biological studies of gene expression in Galleria mellonella have been based on a published transcriptome data set (5), and there was no genome sequence available. However, a genome sequence is crucial to enable homology studies between Galleria mellonella and human, mouse, and other model hosts. Here, we describe the first draft genome sequences available for Galleria mellonella (isolate FT-Tue), based on PacBio technology. Genomic DNA was extracted using the Qiagen Genomic-tip 100/G kit. Sequencing was performed at GATC Biotech AG (Constance, Germany) using PacBio long-read technology. A PacBio standard genomic library was sequenced on 22 single-molecule real-time (SMRT) cells. After subread filtering, this resulted in a total of 20,638,932,410 bases, 2,141,900 reads, an N50 read length of 13,454 bp, and a mean length of 9,635 bp. Our assembly of the genome produced 1,937 contigs comprising 578 Mbp, with an N50 of 952 kbp. The largest contig was 8.98 Mbp.
This first draft genome sequence will help to promote the use of G. mellonella as a replacement organism for vertebrates in biomedical research.
Accession number(s).This whole-genome shotgun project has been deposited at DDBJ/ENA/GenBank under the accession number NTHM00000000. The version described in this paper is version NTHM01000000.
ACKNOWLEDGMENTS
This work was supported by GV-SOLAS (Society of Laboratory Animal Science), the DFG (grants DFG FR 2087/6-1, DFG FR 2087/8-1, CRC685, and SPP1656), DFG Research Training Group 1708, the Bundesministerium für Bildung und Forschung (BMBF), and the German Center for Infection Research (DZIF).
FOOTNOTES
- Received 27 September 2017.
- Accepted 10 November 2017.
- Published 11 January 2018.
- Copyright © 2018 Lange et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
