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Prokaryotes

Draft Genome Sequences of the Obligatory Marine Myxobacterial Strains Enhygromyxa salina SWB005 and SWB007

Jamshid Amiri Moghaddam, Anja Poehlein, Katja Fisch, Mohammad Alanjary, Rolf Daniel, Gabriele M. König, Till F. Schäberle
Jamshid Amiri Moghaddam
Institute for Pharmaceutical Biology, University of Bonn, Bonn, Germany
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Anja Poehlein
Department of Genomics and Applied Microbiology and Göttingen Genomics Laboratory, Georg-August-University Göttingen, Göttingen, Germany
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Katja Fisch
Institute for Insect Biotechnology, Justus Liebig University Giessen, Giessen, Germany
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Mohammad Alanjary
Department of Microbiology and Biotechnology, University of Tübingen, Tübingen, Germany
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Rolf Daniel
Department of Genomics and Applied Microbiology and Göttingen Genomics Laboratory, Georg-August-University Göttingen, Göttingen, Germany
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Gabriele M. König
Institute for Pharmaceutical Biology, University of Bonn, Bonn, GermanyGerman Center for Infection Research (DZIF), Partner Site Cologne-Bonn, Bonn, Germany
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Till F. Schäberle
Institute for Insect Biotechnology, Justus Liebig University Giessen, Giessen, GermanyGerman Center for Infection Research (DZIF), Partner Site Cologne-Bonn, Bonn, Germany
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DOI: 10.1128/genomeA.00324-18
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ABSTRACT

The two marine myxobacterial strains Enhygromyxa salina SWB005 and SWB007 were isolated from coastal soil samples using Escherichia coli as bait for these predatory strains. These strains produce unique specialized metabolites. Genomes were assembled into 312 contigs for E. salina SWB005 (9.0 Mbp) and 192 contigs for E. salina SWB007 (10.6 Mbp).

GENOME ANNOUNCEMENT

Enhygromyxa salina is a marine myxobacterium, and strains of this species have been isolated from different locations around the globe (1, 2). However, to date, only a few obligatory marine myxobacteria have been isolated, and only one genome was available (i.e., from the strain E. salina DSM 15201). Like other myxobacteria, these genomes are large, ranging from 8 to 10 Mbp, and harbor many putative biosynthetic gene clusters (BGCs) coding for the production of specialized metabolites. The strains analyzed here are producers of the natural products salimabromide, salimyxins, and enhygrolides (3–5). E. salina strains SWB005 and SWB007 were isolated from marine sediments from the coast of Santa Barbara, CA (SWB005), and Prerow, Germany (SWB007) (2, 3).

Genomic DNA of both E. salina strains were extracted from fruiting bodies, which appeared after several days of fermentation in artificial seawater (ASW) VY/4 liquid medium (6). DNA was isolated using the GenElute bacterial genomic DNA kit (Sigma-Aldrich). Extracted DNA was used to generate Illumina shotgun paired-end sequencing libraries, which were sequenced with a MiSeq instrument and the MiSeq reagent kit version 3, as recommended by the manufacturer (Illumina, San Diego, CA, USA). Quality filtering using Trimmomatic version 0.36 (7) resulted in 3,773,950 and 3,458,266 paired-end reads for E. salina strains SWB007 and SWB005, respectively.

Assembly resulted in 192 contigs (>500 bp) with an average coverage of 73-fold for E. salina SWB007 and 312 contigs (>500 bp) with an average coverage of 75-fold for E. salina SWB005. The assemblies were validated and the read coverage was determined with Qualimap version 2.1 (8). The resulting draft genomes are 10,602,813 bp (E. salina SWB007) and 9,010,436 bp (E. salina SWB005) in length, and the G+C contents are 68.1% and 69.5% (difference, 1.4%), respectively. Automatic annotation and identification of rRNA and tRNA genes were performed using the software tool Prokka (9). This yielded 2 rRNA genes, 78 tRNA genes, 3,682 protein-encoding genes with function prediction, and 3,265 genes coding for hypothetical proteins for strain SWB005 and 4 rRNA genes, 57 tRNA genes, 4,253 protein-encoding genes with function prediction, and 3,987 genes coding for hypothetical proteins for strain SWB007.

In silico DNA-DNA hybridization (isDDH) was performed based on the identities/high-scoring segment pairs (HSP) length formula (10) and produced a value of 26.10% (+23.8%, −28.6%), which failed the isDDH cutoff of ≥70% that would have determined them to be the same species. The average nucleotide identity (ANI) was calculated to be 78.7%. Therefore, the in silico parameters ANI, isDDH, and difference of the G+C values define these strains as two distinct species of the genus Enhygromyxa.

Using the antiSMASH version 4.0.0 tool (11) for the analysis of the genomes revealed 40 BGCs in E. salina SWB005 and 46 BGCs in E. salina SWB007, indicating the high potential of these bacteria for biosynthesis of specialized metabolites.

Accession number(s).This whole-genome shotgun project has been deposited at DDBJ/ENA/GenBank under the accession numbers PVNK00000000 (E. salina SWB005) and PVNL00000000 (E. salina SWB007). The versions described in this paper are versions PVNK01000000 and PVNL01000000, respectively.

ACKNOWLEDGMENTS

J.A.M. was funded by a fellowship from the Ministry of Science, Research and Technology, Iran. Work in the labs of G.M.K. and T.F.S. was funded by the German Centre for Infection Research (DZIF) through grant TTU09.811 and by the German Federal Ministry of Education and Research (BMBF) through grant 16GW0117K. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Sequencing was performed by the Göttingen Genomics Laboratory (G2L). We thank Melanie Heinemann for technical support.

FOOTNOTES

    • Received 15 March 2018.
    • Accepted 19 March 2018.
    • Published 26 April 2018.
  • Copyright © 2018 Amiri Moghaddam et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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Draft Genome Sequences of the Obligatory Marine Myxobacterial Strains Enhygromyxa salina SWB005 and SWB007
Jamshid Amiri Moghaddam, Anja Poehlein, Katja Fisch, Mohammad Alanjary, Rolf Daniel, Gabriele M. König, Till F. Schäberle
Genome Announcements Apr 2018, 6 (17) e00324-18; DOI: 10.1128/genomeA.00324-18

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Draft Genome Sequences of the Obligatory Marine Myxobacterial Strains Enhygromyxa salina SWB005 and SWB007
Jamshid Amiri Moghaddam, Anja Poehlein, Katja Fisch, Mohammad Alanjary, Rolf Daniel, Gabriele M. König, Till F. Schäberle
Genome Announcements Apr 2018, 6 (17) e00324-18; DOI: 10.1128/genomeA.00324-18
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