Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Microbiology Resource Announcements
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Latest Articles
    • Archive
  • Types of Resources
    • Amplicon Sequence Collections
    • Culture Collections/Mutant Libraries
    • Databases and Software
    • Omics Data Sets
    • Other Genetic Resources
    • Genome Sequences
  • For Authors
    • Getting Started
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About MRA
    • Editor in Chief
    • Board of Editors
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Microbiology Resource Announcements
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Microbiology Resource Announcements
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Latest Articles
    • Archive
  • Types of Resources
    • Amplicon Sequence Collections
    • Culture Collections/Mutant Libraries
    • Databases and Software
    • Omics Data Sets
    • Other Genetic Resources
    • Genome Sequences
  • For Authors
    • Getting Started
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About MRA
    • Editor in Chief
    • Board of Editors
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
Prokaryotes

Draft Genome Sequence of Aeromonas lusitana sp. nov. Strain DSM 24905T, Isolated from a Hot Spring in Vila-Real, Portugal

S. M. Colston, A. Navarro, A. J. Martinez-Murcia, J. Graf
S. M. Colston
aDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
A. Navarro
bGenetic Analysis Strategies S.L., CEEI, Elche, Alicante, Spain
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
A. J. Martinez-Murcia
bGenetic Analysis Strategies S.L., CEEI, Elche, Alicante, Spain
cArea de Microbiología, EPSO, Universidad Miguel Hernández, Orihuela, Alicante, Spain
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J. Graf
aDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for J. Graf
DOI: 10.1128/genomeA.00226-18
  • Article
  • Info & Metrics
  • PDF
Loading

ABSTRACT

Aeromonas lusitana sp. nov. is an isolate derived from a study aimed at characterizing Aeromonas spp. from water sources used for recreation and agricultural purposes and assessing the implications these organisms have for human and animal health. We present here the 4.52-Mbp draft genome sequence of this novel species.

GENOME ANNOUNCEMENT

Members of the genus Aeromonas collectively occupy diverse niches ranging from free-living states in the environment to close associations with animals, sometimes causing disease in their hosts (1, 2). In the environment, Aeromonas spp. are most commonly associated with aquatic habitats, and as such, water sources are of particular interest, since they represent potential contamination routes that may impact human and animal health and disease. Some Aeromonas spp. are also implicated in opportunistic infections in humans; the majority of these have been identified as A. hydrophila, A. veronii, and A. caviae (3), although as classification of the species within this genus continues to improve, other aeromonad species, such as A. dhakensis, have also been suggested to be important infectious agents (4–6). To date, there are 31 established or proposed species of Aeromonas (7).

Aeromonas lusitana sp. nov. strain DSM 24905T (=CECT 7828T, =11/6T, =MDC 2473) was isolated from a hot spring water sample from the Vila Real region of Portugal as part of a study to determine the ubiquity and diversity of Aeromonas species found in unmonitored agricultural and recreational water sources, with an emphasis on determining the antibiotic resistance and virulence factor profile of these aeromonads (8). Further characterization indicated that the isolate was likely from a novel Aeromonas species (9, 10). Genomic DNA was extracted using a thermal shock treatment and RNase A digestion and was purified by elution in DNase/RNase-free water through a silica column. Species identity was confirmed by partial sequencing of the 16S rRNA gene. Libraries were prepared using a Nextera XT kit (Illumina). The libraries were sequenced on the Illumina MiSeq platform, generating a total of 5,570,886 250-bp paired-end reads. CLC Genomics Workbench v10.1 (CLC Bio, Qiagen) was used to filter, trim, and assemble the reads de novo, generating 68 scaffolded contigs, with an N50 value of 148,742 and a coverage of 232×. The total genome size is 4,521,913 bp, with a G+C content of 61.0%. Automated annotation using the NCBI Prokaryotic Genome Annotation Pipeline predicted 4,046 coding sequences and 112 RNA genes. Analysis of the average nucleotide identity with that of other Aeromonas species indicated that the most closely related species is A. tecta (at 89.2%) (11).

Examining the genome of A. lusitana DSM 24905T reveals the strain’s potential versatility in its metabolic capability, which includes chitin and amino sugar catabolism, an arginine deiminase pathway, and organic sulfur utilization. A. lusitana DSM 24905T also encodes general secretion systems and type III and VI secretion pathways, aerolysin, and other putative toxins that may be important factors in host colonization and virulence determination (12–14). The genome contains few mobile elements and insertion sequences (IS5) and two loci with phage-related elements (15, 16). The addition of this genome sequence will provide insight into the phylogeny and diversity of Aeromonas species and into the determinants that result in pathogenic outcomes.

Accession number(s).This whole-genome shotgun project has been deposited at DDBJ/ENA/GenBank under the accession number PGCP00000000. The version described in this paper is version PGCP01000000.

ACKNOWLEDGMENT

This work was funded by USDA-ARS agreement 58-1930-4-002.

FOOTNOTES

    • Received 21 February 2018.
    • Accepted 16 March 2018.
    • Published 12 April 2018.
  • Copyright © 2018 Colston et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

REFERENCES

  1. 1.↵
    1. Janda JM,
    2. Abbott SL
    . 2010. The genus Aeromonas: taxonomy, pathogenicity, and infection. Clin Microbiol Rev 23:35–73. doi:10.1128/CMR.00039-09.
    OpenUrlAbstract/FREE Full Text
  2. 2.↵
    1. Parker JL,
    2. Shaw JG
    . 2011. Aeromonas spp. clinical microbiology and disease. J Infect 62:109–118. doi:10.1016/j.jinf.2010.12.003.
    OpenUrlCrossRefPubMedWeb of Science
  3. 3.↵
    1. Chuang HC,
    2. Ho YH,
    3. Lay CJ,
    4. Wang LS,
    5. Tsai YS,
    6. Tsai CC
    . 2011. Different clinical characteristics among Aeromonas hydrophila, Aeromonas veronii biovar sobria and Aeromonas caviae monomicrobial bacteremia. J Korean Med Sci 26:1415–1420. doi:10.3346/jkms.2011.26.11.1415.
    OpenUrlCrossRefPubMed
  4. 4.↵
    1. Colston SM,
    2. Fullmer MS,
    3. Beka L,
    4. Lamy B,
    5. Gogarten JP,
    6. Graf J
    . 2014. Bioinformatic genome comparisons for taxonomic and phylogenetic assignments using Aeromonas as a test case. mBio 5:e02136. doi:10.1128/mBio.02136-14.
    OpenUrlCrossRefPubMed
  5. 5.↵
    1. Chen PL,
    2. Lamy B,
    3. Ko WC
    . 2016. Aeromonas dhakensis, an increasingly recognized human pathogen. Front Microbiol 7:793. doi:10.3389/fmicb.2016.00793.
    OpenUrlCrossRef
  6. 6.↵
    1. Chen PL,
    2. Wu CJ,
    3. Tsai PJ,
    4. Tang HJ,
    5. Chuang YC,
    6. Lee NY,
    7. Lee CC,
    8. Li CW,
    9. Li MC,
    10. Chen CC,
    11. Tsai HW,
    12. Ou CC,
    13. Chen CS,
    14. Ko WC
    . 2014. Virulence diversity among bacteremic Aeromonas isolates: ex vivo, animal, and clinical evidences. PLoS One 9:e111213. doi:10.1371/journal.pone.0111213.
    OpenUrlCrossRef
  7. 7.↵
    1. Beaz-Hidalgo R,
    2. Latif-Eugenín F,
    3. Hossain MJ,
    4. Berg K,
    5. Niemi RM,
    6. Rapala J,
    7. Lyra C,
    8. Liles MR,
    9. Figueras MJ
    . 2015. Aeromonas aquatica sp. nov., Aeromonas finlandiensis sp. nov. and Aeromonas lacus sp. nov. isolated from Finnish waters associated with cyanobacterial blooms. Syst Appl Microbiol 38:161–168. doi:10.1016/j.syapm.2015.02.005.
    OpenUrlCrossRef
  8. 8.↵
    1. Carvalho MJ,
    2. Martínez-Murcia A,
    3. Esteves AC,
    4. Correia A,
    5. Saavedra MJ
    . 2012. Phylogenetic diversity, antibiotic resistance and virulence traits of Aeromonas spp. from untreated waters for human consumption. Int J Food Microbiol 159:230–239. doi:10.1016/j.ijfoodmicro.2012.09.008.
    OpenUrlCrossRefPubMed
  9. 9.↵
    1. Martínez-Murcia A,
    2. Beaz-Hidalgo R,
    3. Navarro A,
    4. Carvalho MJ,
    5. Aravena-Román M,
    6. Correia A,
    7. Figueras MJ,
    8. Saavedra MJ
    . 2016. Aeromonas lusitana sp. nov., isolated from untreated water and vegetables. Curr Microbiol 72:795–803. doi:10.1007/s00284-016-0997-9.
    OpenUrlCrossRef
  10. 10.↵
    1. Martinez-Murcia AJ,
    2. Monera A,
    3. Saavedra MJ,
    4. Oncina R,
    5. Lopez-Alvarez M,
    6. Lara E,
    7. Figueras MJ
    . 2011. Multilocus phylogenetic analysis of the genus Aeromonas. Syst Appl Microbiol 34:189–199. doi:10.1016/j.syapm.2010.11.014.
    OpenUrlCrossRefPubMed
  11. 11.↵
    1. Goris J,
    2. Konstantinidis KT,
    3. Klappenbach JA,
    4. Coenye T,
    5. Vandamme P,
    6. Tiedje JM
    . 2007. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 57:81–91. doi:10.1099/ijs.0.64483-0.
    OpenUrlCrossRefPubMedWeb of Science
  12. 12.↵
    1. Silver AC,
    2. Kikuchi Y,
    3. Fadl AA,
    4. Sha J,
    5. Chopra AK,
    6. Graf J
    . 2007. Interaction between innate immune cells and a bacterial type III secretion system in mutualistic and pathogenic associations. Proc Natl Acad Sci U S A 104:9481–9486. doi:10.1073/pnas.0700286104.
    OpenUrlAbstract/FREE Full Text
  13. 13.↵
    1. Silver AC,
    2. Rabinowitz NM,
    3. Küffer S,
    4. Graf J
    . 2007. Identification of Aeromonas veronii genes required for colonization of the medicinal leech, Hirudo verbana. J Bacteriol 189:6763–6772. doi:10.1128/JB.00685-07.
    OpenUrlAbstract/FREE Full Text
  14. 14.↵
    1. Suarez G,
    2. Sierra JC,
    3. Erova TE,
    4. Sha J,
    5. Horneman AJ,
    6. Chopra AK
    . 2010. A type VI secretion system effector protein, VgrG1, from Aeromonas hydrophila that induces host cell toxicity by ADP ribosylation of actin. J Bacteriol 192:155–168. doi:10.1128/JB.01260-09.
    OpenUrlAbstract/FREE Full Text
  15. 15.↵
    1. Arndt D,
    2. Grant JR,
    3. Marcu A,
    4. Sajed T,
    5. Pon A,
    6. Liang Y,
    7. Wishart DS
    . 2016. PHASTER: a better, faster version of the PHAST phage search tool. Nucleic Acids Res 44:W16–W21. doi:10.1093/nar/gkw387.
    OpenUrlCrossRefPubMed
  16. 16.↵
    1. Zhou Y,
    2. Liang Y,
    3. Lynch KH,
    4. Dennis JJ,
    5. Wishart DS
    . 2011. PHAST: a fast phage search tool. Nucleic Acids Res 39:W347–W352. doi:10.1093/nar/gkr485.
    OpenUrlCrossRefPubMedWeb of Science
PreviousNext
Back to top
Download PDF
Citation Tools
Draft Genome Sequence of Aeromonas lusitana sp. nov. Strain DSM 24905T, Isolated from a Hot Spring in Vila-Real, Portugal
S. M. Colston, A. Navarro, A. J. Martinez-Murcia, J. Graf
Genome Announcements Apr 2018, 6 (15) e00226-18; DOI: 10.1128/genomeA.00226-18

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print
Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Microbiology Resource Announcements article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Draft Genome Sequence of Aeromonas lusitana sp. nov. Strain DSM 24905T, Isolated from a Hot Spring in Vila-Real, Portugal
(Your Name) has forwarded a page to you from Microbiology Resource Announcements
(Your Name) thought you would be interested in this article in Microbiology Resource Announcements.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Draft Genome Sequence of Aeromonas lusitana sp. nov. Strain DSM 24905T, Isolated from a Hot Spring in Vila-Real, Portugal
S. M. Colston, A. Navarro, A. J. Martinez-Murcia, J. Graf
Genome Announcements Apr 2018, 6 (15) e00226-18; DOI: 10.1128/genomeA.00226-18
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • GENOME ANNOUNCEMENT
    • ACKNOWLEDGMENT
    • FOOTNOTES
    • REFERENCES
  • Info & Metrics
  • PDF

Related Articles

Cited By...

About

  • About MRA
  • Editor in Chief
  • Board of Editors
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • Getting Started
  • Submit a Manuscript
  • Author Warranty
  • Ethics
  • Contact Us
  • ASM Author Center

Follow #MRAJournal

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Online ISSN: 2576-098X