Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Microbiology Resource Announcements
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Latest Articles
    • Archive
  • Types of Resources
    • Amplicon Sequence Collections
    • Culture Collections/Mutant Libraries
    • Databases and Software
    • Omics Data Sets
    • Other Genetic Resources
    • Genome Sequences
  • For Authors
    • Getting Started
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About MRA
    • Editor in Chief
    • Board of Editors
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Microbiology Resource Announcements
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Microbiology Resource Announcements
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Latest Articles
    • Archive
  • Types of Resources
    • Amplicon Sequence Collections
    • Culture Collections/Mutant Libraries
    • Databases and Software
    • Omics Data Sets
    • Other Genetic Resources
    • Genome Sequences
  • For Authors
    • Getting Started
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About MRA
    • Editor in Chief
    • Board of Editors
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
Viruses

Full-Genome Sequence of the First G8P[14] Rotavirus Strain Detected in the United States

Slavica Mijatovic-Rustempasic, Sunando Roy, Michele Sturgeon, Kunchala Rungsrisuriyachai, Erik Reisdorf, Margaret M. Cortese, Michael D. Bowen
Slavica Mijatovic-Rustempasic
aDivision of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sunando Roy
aDivision of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michele Sturgeon
aDivision of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kunchala Rungsrisuriyachai
aDivision of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Erik Reisdorf
bWisconsin State Laboratory of Hygiene, Madison, Wisconsin, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Margaret M. Cortese
aDivision of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael D. Bowen
aDivision of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/genomeA.00677-15
  • Article
  • Info & Metrics
  • PDF
Loading

ABSTRACT

This is a report of the complete genomic sequence of a rare rotavirus group A G8-P[14]-I2-R3-C2-M2-A3-N2-T6-E2-H3 strain detected in a stool sample from a 57-year-old subject.

GENOME ANNOUNCEMENT

Group A rotaviruses (RVA) are a major etiologic agent of gastroenteritis in infants and young children worldwide (1). Among adults, RVA infections have been associated with nonseasonal outbreaks, with a wide spectrum of disease severity (2). The RVA genomic classification nomenclature is based on all 11 segments of double-stranded RNA (dsRNA) encoding the VP7, VP4, VP6, VP1-3, and NSP1-5/6 proteins and uses the notation Gx-P[x]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx, respectively, with “x” indicating the numbers of the corresponding genotypes (3). For a majority of human RVA strains, variation in the backbone genes can be differentiated by three genotype constellations, the Wa-like genogroup 1 (I1-R1-C1-M1-A1-N1-T1-E1-H1), the DS-1-like genogroup 2 (I2-R2-C2-M2-A2-N2-T2-E2-H2), and the AU-1-like genogroup 3 (I3-R3-C3-M3-A3-N3-T3-E3-H3) that are believed to have originated from porcine, bovine, and feline RVAs, respectively (3).

Here, we report the full-genome sequence of RVA strain RVA/Human-wt/USA/2012841174/2012/G8P[14] (2012841174), detected in 2012 in a stool sample from a 57-year-old subject from Wisconsin. The methods used for determining the full-length genome sequence and assignment of genotypes were described previously (4). The sizes of full-length segments 1 to 11 were 3,302, 2,684, 2,591, 2,362, 1,578, 1,356, 1,075, 1,059, 1,062, 751, and 667 bp, respectively, and the open reading frame lengths for these segments were 3,267, 2,643, 2,508, 2,321, 1,476, 1,194, 933, 954, 981, 528, and 597 bp, respectively.

The strain 2012841174 genome constellation is G8-P[14]-I2-R3-C2-M2-A3-N2-T6-E2-H3 and differs from other previously reported G8P[14] strains because the VP1 (R3) and NSP1 (A3) genes are from genogroup 3. Phylogenetic analysis of individual genes revealed a combination of genes of animal and human origin. The VP2, VP3, VP6, NSP1, NSP3, and NSP4 genes share a high degree of nucleotide homology with bovine strains (95.4 to 97.5%), while the VP1, NSP2, and NSP5 genes are closely related to simian strains, with nucleotide similarities ranging from 97.5 to 98.8%. The VP7 and VP4 genes share 87.4% and 96.2% nucleotide identity with human strains WAG8.2 and A64, respectively.

Recent phylogenetic analysis revealed seven distinct lineages among P[14] strains (5), and the P[14] gene of strain 2012841174 falls into lineage 1. G8 RVA strains in combination with P[14] have been known to infect children and cattle in several countries (6–9). There is limited genetic information regarding these strains, since only 4 full-genome sequences for G8P[14] strains have been reported to date. This is the first G8P[14] strain identified in the United States.

Nucleotide sequence accession numbers.The strain 2012841174 gene sequences have been deposited in GenBank under the accession numbers KJ411432 to KJ411442.

ACKNOWLEDGMENTS

We thank M. Leanne Ward for editorial assistance.

Support for this study was provided by CDC program funding.

The findings and conclusions in this report are those of the author(s) and do not necessarily represent the official position of the Centers for Disease Control and Prevention. Names of specific vendors, manufacturers, or products are included for public health and informational purposes; inclusion does not imply endorsement of the vendors, manufacturers, or products by the Centers for Disease Control and Prevention or the U.S. Department of Health and Human Services.

FOOTNOTES

    • Received 19 May 2015.
    • Accepted 19 May 2015.
    • Published 18 June 2015.
  • Copyright © 2015 Mijatovic-Rustempasic et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.

REFERENCES

  1. 1.↵
    1. Tate JE,
    2. Cortese MM,
    3. Payne DC,
    4. Curns AT,
    5. Yen C,
    6. Esposito DH,
    7. Cortes JE,
    8. Lopman BA,
    9. Patel MM,
    10. Gentsch JR,
    11. Parashar UD
    . 2011. Uptake, impact, and effectiveness of rotavirus vaccination in the United States: review of the first 3 years of postlicensure data. Pediatr Infect Dis J 30(1 Suppl):S56–S60. doi:10.1097/INF.0b013e3181fefdc0.
    OpenUrlCrossRefPubMedWeb of Science
  2. 2.↵
    1. Anderson EJ,
    2. Weber SG
    . 2004. Rotavirus infection in adults. Lancet Infect Dis 4:91–99. doi:10.1016/S1473-3099(04)00928-4.
    OpenUrlCrossRefPubMedWeb of Science
  3. 3.↵
    1. Matthijnssens J,
    2. Ciarlet M,
    3. McDonald SM,
    4. Attoui H,
    5. Bányai K,
    6. Brister JR,
    7. Buesa J,
    8. Esona MD,
    9. Estes MK,
    10. Gentsch JR,
    11. Iturriza-Gómara M,
    12. Johne R,
    13. Kirkwood CD,
    14. Martella V,
    15. Mertens PP,
    16. Nakagomi O,
    17. Parreño V,
    18. Rahman M,
    19. Ruggeri FM,
    20. Saif LJ
    . 2011. Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG). Arch Virol 156:1397–1413. doi:10.1007/s00705-011-1006-z.
    OpenUrlCrossRefPubMed
  4. 4.↵
    1. Mijatovic-Rustempasic S,
    2. Roy S,
    3. Sturgeon M,
    4. Rungsrisuriyachai K,
    5. Esona MD,
    6. Degroat D,
    7. Qin X,
    8. Cortese MM,
    9. Bowen MD
    . 2014. Full-genome sequence of a rare human G3P[9] rotavirus strain. Genome Announc 2(2):e00143-14. doi:10.1128/genomeA.00143-14.
    OpenUrlAbstract/FREE Full Text
  5. 5.↵
    1. Tam KI,
    2. Roy S,
    3. Esona MD,
    4. Jones S,
    5. Sobers S,
    6. Morris-Glasgow V,
    7. Rey-Benito G,
    8. Gentsch JR,
    9. Bowen MD
    . 2014. Full genomic characterization of a novel genotype combination, G4P[14], of a human rotavirus strain from Barbados. Infect Genet Evol 28:524–529. doi:10.1016/j.meegid.2014.09.020.
    OpenUrlCrossRefPubMed
  6. 6.↵
    1. Bányai K,
    2. Papp H,
    3. Dandár E,
    4. Molnár P,
    5. Mihály I,
    6. Van Ranst M,
    7. Martella V,
    8. Matthijnssens J
    . 2010. Whole genome sequencing and phylogenetic analysis of a zoonotic human G8P[14] rotavirus strain. Infect Genet Evol 10:1140–1144. doi:10.1016/j.meegid.2010.05.001.
    OpenUrlCrossRefPubMed
  7. 7.↵
    1. Wu FT,
    2. Bányai K,
    3. Wu HS,
    4. Yang DC,
    5. Lin JS,
    6. Hsiung CA,
    7. Huang YC,
    8. Hwang KP,
    9. Jiang B,
    10. Gentsch JR
    . 2012. Identification of a G8P[14] rotavirus isolate obtained from a Taiwanese child: evidence for a relationship with bovine rotaviruses. Jpn J Infect Dis 65:455–457. doi:10.7883/yoken.65.455.
    OpenUrlCrossRefPubMed
  8. 8.↵
    1. Chitambar SD,
    2. Arora R,
    3. Kolpe AB,
    4. Yadav MM,
    5. Raut CG
    . 2011. Molecular characterization of unusual bovine group A rotavirus G8P[14] strains identified in western India: emergence of P[14] genotype. Vet Microbiol 148:384–388. doi:10.1016/j.vetmic.2010.08.027.
    OpenUrlCrossRefPubMed
  9. 9.↵
    1. Medici MC,
    2. Abelli LA,
    3. Martinelli M,
    4. Dettori G,
    5. Chezzi C
    . 2008. Molecular characterization of VP4, VP6 and VP7 genes of a rare G8P[14] rotavirus strain detected in an infant with gastroenteritis in Italy. Virus Res 137:163–167. doi:10.1016/j.virusres.2008.07.005.
    OpenUrlCrossRefPubMed
View Abstract
PreviousNext
Back to top
Download PDF
Citation Tools
Full-Genome Sequence of the First G8P[14] Rotavirus Strain Detected in the United States
Slavica Mijatovic-Rustempasic, Sunando Roy, Michele Sturgeon, Kunchala Rungsrisuriyachai, Erik Reisdorf, Margaret M. Cortese, Michael D. Bowen
Genome Announcements Jun 2015, 3 (3) e00677-15; DOI: 10.1128/genomeA.00677-15

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print
Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Microbiology Resource Announcements article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Full-Genome Sequence of the First G8P[14] Rotavirus Strain Detected in the United States
(Your Name) has forwarded a page to you from Microbiology Resource Announcements
(Your Name) thought you would be interested in this article in Microbiology Resource Announcements.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Full-Genome Sequence of the First G8P[14] Rotavirus Strain Detected in the United States
Slavica Mijatovic-Rustempasic, Sunando Roy, Michele Sturgeon, Kunchala Rungsrisuriyachai, Erik Reisdorf, Margaret M. Cortese, Michael D. Bowen
Genome Announcements Jun 2015, 3 (3) e00677-15; DOI: 10.1128/genomeA.00677-15
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • GENOME ANNOUNCEMENT
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Info & Metrics
  • PDF

Related Articles

Cited By...

About

  • About MRA
  • Editor in Chief
  • Board of Editors
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • Getting Started
  • Submit a Manuscript
  • Author Warranty
  • Ethics
  • Contact Us
  • ASM Author Center

Follow #MRAJournal

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Online ISSN: 2576-098X