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Archive

January 2021; Volume 10,Issue 1

Genome Sequences

  • Draft Genome Sequence of <em>Fusarium</em> sp. Strain DS 682, a Novel Fungal Isolate from the Grass Rhizosphere
    Genome Sequences
    Draft Genome Sequence of Fusarium sp. Strain DS 682, a Novel Fungal Isolate from the Grass Rhizosphere

    The novel fungal strain, Fusarium sp. strain DS 682, was isolated from the rhizosphere of the perennial grass, Bouteloua gracilis, at the Konza Prairie Biological Station in Kansas. This fungal strain is common across North American grasslands and is resilient to environmental fluctuations. The draft genome is estimated to be 97.2% complete.

    Arunima Bhattacharjee, Lindsey N. Anderson, Trinidad Alfaro, Andrea Porras-Alfaro, Ari Jumpponen, Kirsten S. Hofmockel, Janet K. Jansson, Christopher R. Anderton, William C. Nelson
    7 Jan 2021
  • Whole-Genome Sequencing of Two Moroccan <span class="named-content genus-species" id="named-content-1">Mycobacterium tuberculosis</span> Strains
    Genome Sequences
    Whole-Genome Sequencing of Two Moroccan Mycobacterium tuberculosis Strains

    Mycobacterium tuberculosis is known to cause pulmonary and extrapulmonary tuberculosis. In Morocco, the spread of multidrug-resistant (MDR) tuberculosis (TB) has become a major challenge. Here, we announce the draft genome sequences of two Mycobacterium tuberculosis strains, MTB1 and MTB2, isolated...

    M. Laamarti, N. El Mrimar, T. Alouane, S. Kartti, E. Belouad, F. Bssaibis, A. Zegmout, R. El Jaoudi, A. Maleb, A. Abid, N. El Hajjami, A. Lemnouer, S. Siah, L. Belyamani, M. Elouennass, A. Ibrahimi
    7 Jan 2021
  • Characterization of a Complete Genome Sequence of Molluscum Contagiosum Virus from an Adult Woman in Australia
    Genome Sequences
    Characterization of a Complete Genome Sequence of Molluscum Contagiosum Virus from an Adult Woman in Australia

    The complete genome sequence of molluscum contagiosum virus 1 (MOCV1) isolate NT2017 was sequenced from a tissue sample from an Australian woman. The genome consisted of 185,655 bp encoding 169 predicted open reading frames. Phylogenetically, isolate NT2017 was most closely related to an MOCV1 strain from Slovenia.

    Subir Sarker, Sally R. Isberg, Ajani Athukorala, Ravi Mathew, Nolasco Capati, Md. Hakimul Haque, Karla J. Helbig
    7 Jan 2021
  • Coding-Complete Genome Sequences of Emerging Rabbit Hemorrhagic Disease Virus Type 2 Isolates Detected in 2020 in the United States
    Genome Sequences
    Coding-Complete Genome Sequences of Emerging Rabbit Hemorrhagic Disease Virus Type 2 Isolates Detected in 2020 in the United States

    Five rabbit hemorrhagic disease virus type 2 (RHDV2) coding-complete genome sequences were obtained from the livers of domestic and wild rabbits during the 2020 outbreak in the United States. These represent the first available RHDV2 sequences from the United States.

    V. K. O’Donnell, L. Xu, K. Moran, F. Mohamed, T. Boston, S. J. Pauszek, D. A. Vierra, B. Faburay, K. A. Dodd, R. W. Barrette
    7 Jan 2021
  • Draft Genome Sequence of NYR20, a Red Pigment-Secreting Mutant of <span class="named-content genus-species" id="named-content-1">Saccharomyces cerevisiae</span>
    Genome Sequences
    Draft Genome Sequence of NYR20, a Red Pigment-Secreting Mutant of Saccharomyces cerevisiae

    A Saccharomyces cerevisiae mutant strain, NYR20, produces a red pigment owing to adenine auxotrophy. Unlike other yeast adenine biosynthetic mutants, this strain not only produces but also secretes this pigment. Here, we report the NYR20 draft genome sequence, thereby advancing our understanding of pigment secretion mechanisms.

    Hiro Takahashi, Shin-ichi Iwaguchi, Hisashi Kondo, Taichiro Motomura, Masataka Murase, Anna Takahashi, Shuichi Fukuyoshi, Chiyoko Machida, Shin Kanamasa, Satoru Yamamoto, Takayuki Yoshizaki
    7 Jan 2021
  • Complete Genome Sequence of Strain SS-5, a Magnetotactic Gammaproteobacterium Isolated from the Salton Sea, a Shallow, Saline, Endorheic Rift Lake Located on the San Andreas Fault in California
    Genome Sequences
    Complete Genome Sequence of Strain SS-5, a Magnetotactic Gammaproteobacterium Isolated from the Salton Sea, a Shallow, Saline, Endorheic Rift Lake Located on the San Andreas Fault in California

    We report the 3.7-Mb genome sequence of strain SS-5, a magnetotactic, sulfur-oxidizing rod and member of the family Chromatiaceae of the class Gammaproteobacteria, which biomineralizes membrane-bounded, elongated, prismatic octahedral, magnetite nanocrystals. This genome sequence brings further diversity for understanding the origin and evolution of magnetotaxis and magnetosome biomineralization.

    Denis Trubitsyn, Caroline L. Monteil, Corey Geurink, Viviana Morillo-Lopez, Luiz Gonzaga Paula de Almeida, Ana Tereza Ribeiro de Vasconcelos, Fernanda Abreu, Dennis A. Bazylinski, Christopher T. Lefevre
    7 Jan 2021
  • Draft Genome Sequences of Three Strains of <span class="named-content genus-species" id="named-content-1">Pseudomonas syringae</span> pv. eriobotryae, a Pathogen Causing Canker Disease in Loquat, Isolated in Japan
    Genome Sequences
    Draft Genome Sequences of Three Strains of Pseudomonas syringae pv. eriobotryae, a Pathogen Causing Canker Disease in Loquat, Isolated in Japan

    The phytopathogenic bacterium Pseudomonas syringae pv. eriobotryae causes canker disease in loquat. Isolates from Japan are classified into three groups based on pathogenicity and pigment production. In this study, we report the draft genome sequences of three strains, one belonging to each of the three groups.

    Hiroaki Tashiro, Yukio Nagano, Ayaka Jiromaru, Ryunosuke Sakaguchi, Naofumi Hiehata, Shinji Fukuda
    7 Jan 2021
  • Complete Annotated Genome Sequence of <span class="named-content genus-species" id="named-content-1">Limosilactobacillus fermentum</span> AGR1487
    Genome Sequences
    Complete Annotated Genome Sequence of Limosilactobacillus fermentum AGR1487

    Limosilactobacillus fermentum is a probiotic species; however, L. fermentum AGR1487 increases colon inflammation in germfree mice and decreases barrier integrity in Caco-2 cells. The AGR1487 genome was sequenced to explore these phenotypes. The genome is a single, circular, 1,939,032-bp chromosome with a G+C content of 52.17% and no plasmids.

    ...
    Marc A. Bailie, Eric Altermann, Wayne Young, Nicole C. Roy, Warren C. McNabb
    7 Jan 2021
  • Draft Genome Sequence of <em>Pseudomonas</em> sp. Strain T2.31D-1, Isolated from a Drilling Core Sample Obtained 414 Meters below Surface in the Iberian Pyrite Belt
    Genome Sequences
    Draft Genome Sequence of Pseudomonas sp. Strain T2.31D-1, Isolated from a Drilling Core Sample Obtained 414 Meters below Surface in the Iberian Pyrite Belt

    We report the draft genome of Pseudomonas sp. strain T2.31D-1, which was isolated from a drilling core sample obtained 414 m below surface in the Iberian Pyrite Belt. The genome consists of a 4.7-Mb chromosome, with 4,428 coding sequences, one rRNA operon, and 59 tRNA genes, and a 31.8-kb plasmid.

    J. M. Martínez, C. Escudero, T. Leandro, G. Mateos, R. Amils
    7 Jan 2021
  • Complete Genome Sequence of <span class="named-content genus-species" id="named-content-1">Streptococcus mitis</span> Strain Nm-65, Isolated from a Patient with Kawasaki Disease
    Genome Sequences
    Complete Genome Sequence of Streptococcus mitis Strain Nm-65, Isolated from a Patient with Kawasaki Disease

    Streptococcus mitis Nm-65 is a human commensal streptococcal strain of the mitis group that was isolated from the tooth surface of a patient with Kawasaki disease. The complete genome sequence of Nm-65 was obtained by means of hybrid assembly, using two next-generation sequencing data sets. The final assembly size was 2,085,837 bp, with 2,039 coding sequences.

    ...
    Atsushi Tabata, Hisashi Ohkuni, Yasuhiko Itoh, Yoshitaka Fukunaga, Toshifumi Tomoyasu, Hideaki Nagamune
    7 Jan 2021
  • Draft Genome Sequences of Six <em>Vibrio</em> Strains Isolated from the Atlantic Intertidal Marine Sponge <span class="named-content genus-species" id="named-content-1">Ophlitaspongia papilla</span>
    Genome Sequences
    Draft Genome Sequences of Six Vibrio Strains Isolated from the Atlantic Intertidal Marine Sponge Ophlitaspongia papilla

    We report here the genome sequences of six Vibrio strains isolated from an Atlantic intertidal marine sponge, Ophlitaspongia papilla. Genome mining and comparative genomics will assist in deciphering the bioactive potential of the symbiotic microbes and molecular mechanisms of sponge-microbial symbioses.

    Anoop Alex, Vijayakumari Pratheepa, Joana Martins, Agostinho Antunes
    7 Jan 2021
  • Genome Sequence of the Versatile Deadwood Decomposer <span class="named-content genus-species" id="named-content-1">Xylaria grammica</span> IHIA82
    Genome Sequences
    Genome Sequence of the Versatile Deadwood Decomposer Xylaria grammica IHIA82

    Xylaria grammica is an ascomycetous decomposer of dead hardwood. X. grammica IHIA82 was recovered from the Kakamega Forest, Kenya. The whole genome of this strain was sequenced with a total size of 47.0 Mbp, a G+C content of 48.1%, and 12,126 predicted genes.

    Enrico Büttner, Virginia Wambui Kimani, Harald Kellner, Martin Hofrichter, Christiane Liers
    7 Jan 2021
  • Complete Genome Sequence of <em>Streptomyces</em> Phage Salutena
    Genome Sequences
    Complete Genome Sequence of Streptomyces Phage Salutena

    Streptomyces are Gram-negative soil bacteria that can degrade lignin and synthesize antibiotics. Some species cause mycetoma, pneumonitis, and bloodstream infections. Here, we present the genome sequence of the Streptomyces sp. strain Mg1 phage Salutena, a siphovirus in the subfamily Arquatrovirinae. The genome is 51,993 bp, with 90 predicted protein-coding genes.

    Jinha Kim, Tyler Higbee, James Clark, Tram Le, Mei Liu, Ben Burrowes
    7 Jan 2021
  • Complete Genome Sequence of <span class="named-content genus-species" id="named-content-1">Mycobacterium orygis</span> Strain 51145
    Genome Sequences
    Complete Genome Sequence of Mycobacterium orygis Strain 51145

    We report the complete 4,352,172-bp genome sequence of Mycobacterium orygis strain 51145 assembled into a single circular chromosome. Comparative genomic analyses with other lineages of the Mycobacterium tuberculosis complex can provide insights into the biology, evolution, and epidemiology of this...

    Syed Beenish Rufai, Fiona McIntosh, Indira Poojary, Shubhada Chothe, Aswathy Sebastian, Istvan Albert, Craig Praul, Manigandan Venkatesan, Gibarni Mahata, Hindol Maity, Premanshu Dandapat, Joy Sarojini Michael, Robab Katani, Vivek Kapur, Marcel A. Behr
    7 Jan 2021
  • Complete Genome Sequences for 36 Canadian <span class="named-content genus-species" id="named-content-1">Salmonella enterica</span> Serovar Typhimurium and I 1,4,[5],12:i:– Isolates from Clinical and Animal Sources
    Genome Sequences
    Complete Genome Sequences for 36 Canadian Salmonella enterica Serovar Typhimurium and I 1,4,[5],12:i:– Isolates from Clinical and Animal Sources

    Here, we report the complete genome sequences for 36 Canadian isolates of Salmonella enterica subsp. enterica serovar Typhimurium and its monophasic variant I 1,4,[5]:12:i:– from both clinical and animal sources. These genome sequences will provide useful references for understanding the genetic variation within this prominent serotype.

    Justin Schonfeld, Clifford Clark, James Robertson, Gitanjali Arya, Shannon H. C. Eagle, Simone Gurnik, Roger Johnson, Genevieve Labbe, Kira Liu, Shaun Kernaghan, Amanda Mazzocco, Joanne MacKinnon, Kim Ziebell, John H. E. Nash
    7 Jan 2021
  • Genome Sequence of a Dengue Virus Serotype 2 Strain Identified during the 2019 Outbreak in Bangladesh
    Genome Sequences
    Genome Sequence of a Dengue Virus Serotype 2 Strain Identified during the 2019 Outbreak in Bangladesh

    A nearly complete genome sequence of a dengue virus serotype 2 strain detected in the serum of a patient in 2019 during the largest outbreak of dengue fever in Bangladesh is reported.

    Roly Malaker, Mohammad S. I. Sajib, Apurba R. Malaker, Hafizur Rahman, Yogesh Hooda, Md Hasanuzzaman, Samir K. Saha, Senjuti Saha
    7 Jan 2021
  • Draft Genome Sequence of <em>Bacillus</em> sp. Strain E25, a Biocontrol and Plant Growth-Promoting Bacterial Endophyte Isolated from Mexican Husk Tomato Roots (<em>Physalis ixocarpa</em> Brot. Ex Horm.)
    Genome Sequences
    Draft Genome Sequence of Bacillus sp. Strain E25, a Biocontrol and Plant Growth-Promoting Bacterial Endophyte Isolated from Mexican Husk Tomato Roots (Physalis ixocarpa Brot. Ex Horm.)

    Bacillus sp. strain E25 is an endophytic strain isolated from husk tomato roots in Michoacán, México, and displayed excellent biocontrol and plant growth-promoting activities under normal and salt stress conditions. This draft genome report confirms the presence of genes involved in direct and indirect mechanisms to stimulate plant growth and health.

    Adrián Pérez-Equihua, Gustavo Santoyo
    7 Jan 2021
  • Whole-Genome Sequence of Bioactive Compound-Producing <span class="named-content genus-species" id="named-content-1">Pseudomonas aeruginosa</span> Strain LV
    Genome Sequences
    Whole-Genome Sequence of Bioactive Compound-Producing Pseudomonas aeruginosa Strain LV

    Pseudomonas aeruginosa is known for a high adaptive capacity due to the ability to synthesize several compounds that give advantages for competing with other microorganisms in the environment. The LV strain synthesizes bioactive compounds, mainly by secondary metabolism, with antitumor and antimicrobial activities against microbial pathogens.

    Ane Stéfano Simionato, Bárbara Gionco Cano, Miguel Octavio Pérez Navarro, Eliandro Reis Tavares, Renan Augusto Ribeiro, Mariangela Hungria, Lucy Megumi Yamauchi, Sueli Fumie Yamada-Ogatta, Galdino Andrade
    7 Jan 2021
  • Novel Recombinant Foot-and-Mouth Disease Virus Circulating in Vietnam
    Genome Sequences
    Novel Recombinant Foot-and-Mouth Disease Virus Circulating in Vietnam

    We report the genome sequences of 12 recombinant foot-and-mouth disease virus isolates from Vietnam. The recombinant strain has a capsid region from an A/Sea-97 strain and a nonstructural segment from an O/ME-SA/PanAsia strain. The isolates were obtained from two outbreak samples collected in June 2017 and 10 subclinical samples collected between 2017 and 2019.

    Miranda R. Bertram, Barbara Brito, Rachel M. Palinski, Ian H. Fish, Steven J. Pauszek, Ethan J. Hartwig, George R. Smoliga, Le T. Vu, Bui H. Hoang, Nguyen T. Phuong, Vo V. Hung, Pham P. Vu, Nguyen K. Dung, Nguyen N. Tien, Pham V. Dong, Do H. Dung, Jonathan Arzt
    7 Jan 2021
  • Coding-Complete Genome Sequence of <em>Avian orthoavulavirus 16</em>, Isolated from Emperor Goose (<span class="named-content genus-species" id="named-content-1">Anser canagicus</span>) Feces, Alaska, USA
    Genome Sequences
    Coding-Complete Genome Sequence of Avian orthoavulavirus 16, Isolated from Emperor Goose (Anser canagicus) Feces, Alaska, USA

    We sequenced the coding-complete genome of an avian orthoavulavirus serotype 16 (AOAV-16) isolate recovered from emperor goose (Anser canagicus) feces collected in Alaska. The detection of AOAV-16 in North America and genomic sequencing of the resultant isolate confirms that the geographic distribution of this virus extends beyond Asia.

    Andrew B. Reeves, Mary Lea Killian, Michael E. Tanner, Benjamin J. Lagassé, Andrew M. Ramey, David E. Stallknecht, Rebecca L. Poulson
    7 Jan 2021
  • Draft Genome Sequences of Four Commensal Strains of <em>Staphylococcus</em> and <em>Pseudomonas</em> Isolated from Healthy Human Skin
    Genome Sequences
    Draft Genome Sequences of Four Commensal Strains of Staphylococcus and Pseudomonas Isolated from Healthy Human Skin

    Staphylococcus spp. and Pseudomonas spp. are widely distributed bacteria in the environment and are found in association with animals and humans. Here, we present the draft genome sequence data of the healthy human skin commensal strains Staphylococcus aureus MFP03, Staphylococcus...

    Xavier Janvier, Amine M. Boukerb, Marc G. J. Feuilloley, Anne Groboillot
    7 Jan 2021
  • Whole-Genome Shotgun Sequence of <span class="named-content genus-species" id="named-content-1">Bacillus mycoides</span> Strain U53, a Psychrotolerant Bacterium Isolated from the Sakhalin Region in Russia
    Genome Sequences
    Whole-Genome Shotgun Sequence of Bacillus mycoides Strain U53, a Psychrotolerant Bacterium Isolated from the Sakhalin Region in Russia

    The shotgun genome sequence of Bacillus mycoides strain U53, a psychrotolerant bacterium that was isolated from soil in Russia, is characterized. The bacterial genome is 5,710,017 bp long and is predicted to have 5,847 genes. The GC content is 35.4%.

    Ha My T. Phan, Marina L. Sidorenko, Po-Chun Tsai, Shir-Ly Huang
    7 Jan 2021
  • Draft Genome Sequence of Terrestrial <em>Streptomyces</em> sp. Strain VITNK9, Isolated from Vellore, Tamil Nadu, India, Exhibiting Antagonistic Activity against Fish Pathogens
    Genome Sequences
    Draft Genome Sequence of Terrestrial Streptomyces sp. Strain VITNK9, Isolated from Vellore, Tamil Nadu, India, Exhibiting Antagonistic Activity against Fish Pathogens

    We report the draft genome sequence of Streptomyces sp. strain VITNK9, isolated from a soil sample collected in Vellore district (12.9165°N, 79.1325°E), Tamil Nadu, India, with an assembly size of 7,920,076 bp and 72.7% GC content.

    Subhasish Saha, Nabila Mohammad Ishaque, Ilia Burgsdorf, Markéta Macho, Daniela Ewe, Laura Steindler, Pavel Hrouzek, Krishnan Kannabiran, Kumar Saurav
    7 Jan 2021
  • Genome Sequence of the Fungus <span class="named-content genus-species" id="named-content-1">Nannizziopsis barbatae</span>, an Emerging Reptile Pathogen
    Genome Sequences
    Genome Sequence of the Fungus Nannizziopsis barbatae, an Emerging Reptile Pathogen

    Nannizziopsis barbata is an emerging fungal pathogen capable of causing contagious dermatomycosis in reptiles. Here, we report a 31.54-Mb draft genome sequence of an isolate originating from an infected eastern water dragon in Brisbane, Australia.

    Daniel Powell, Ashley Jones, Nicola Kent, Parwinder Kaur, Ido Bar, Benjamin Schwessinger, Céline H. Frère
    7 Jan 2021
  • Coding-Complete Genome Sequences of Six Influenza Type A Strains Circulating in Lithuania in the 2009–2010 Epidemic Season
    Genome Sequences
    Coding-Complete Genome Sequences of Six Influenza Type A Strains Circulating in Lithuania in the 2009–2010 Epidemic Season

    Here, we report the coding-complete genome sequences of six influenza A(H1N1) strains that were detected in Vilnius, Lithuania, among patients exhibiting influenza-like symptoms during the 2009–2010 epidemic season, in a national influenza surveillance. Several mutations were found in genes encoding hemagglutinin and neuraminidase, in comparison with the A/California/07/2009 reference strain (GenBank accession numbers...

    Lukasz Rabalski, Boguslaw Szewczyk, Kęstutis Zagminas, Algirdas Griskevicius, Krzysztof Lepek
    7 Jan 2021
  • Two SARS-CoV-2 Genome Sequences of Isolates from Rural U.S. Patients Harboring the D614G Mutation, Obtained Using Nanopore Sequencing
    Genome Sequences
    Two SARS-CoV-2 Genome Sequences of Isolates from Rural U.S. Patients Harboring the D614G Mutation, Obtained Using Nanopore Sequencing

    Two coding-complete sequences of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were obtained from samples from two patients in Arkansas, in the southeastern corner of the United States. The viral genome was obtained using the ARTIC Network protocol and Oxford Nanopore Technologies sequencing.

    Piroon Jenjaroenpun, Visanu Wanchai, Kikumi D. Ono-Moore, Jennifer Laudadio, Laura P. James, Sean H. Adams, Fred Prior, Intawat Nookaew, David W. Ussery, Thidathip Wongsurawat
    17 Dec 2020
  • Complete Genome Sequence of <em>Rehmannia Mosaic Virus</em> Infecting <span class="named-content genus-species" id="named-content-1">Rehmannia glutinosa</span> in Japan
    Genome Sequences
    Complete Genome Sequence of Rehmannia Mosaic Virus Infecting Rehmannia glutinosa in Japan

    The complete genome sequence of isolate Jiou of rehmannia mosaic virus (ReMV) infecting Rehmannia glutinosa in Japan was obtained via Sanger sequencing. Isolate Jiou shared high nucleotide sequence identity (>94%) with other known ReMV isolates.

    Naoki Komiyama, Kohei Sakuta, Masayuki Mikage, Hirosuke Shinohara, Toru Iwanami, Hiromitsu Negishi, Ok-Kyung Kim
    7 Jan 2021
  • Genome Sequence of <span class="named-content genus-species" id="named-content-1">Acetobacter tropicalis</span> DmPark25_167, a Bacterium Isolated from a <span class="named-content genus-species" id="named-content-2">Drosophila melanogaster</span> Genetic Model of Parkinson’s Disease
    Genome Sequences
    Genome Sequence of Acetobacter tropicalis DmPark25_167, a Bacterium Isolated from a Drosophila melanogaster Genetic Model of Parkinson’s Disease

    Here, we report the genome of Acetobacter tropicalis DmPark25_167, a bacterial strain isolated from a Drosophila melanogaster park25 mutant. The park25 mutant is an established genetic model of Parkinson’s disease. DmPark25_167 has duplicated methionine...

    Julie A. Goddard, Samara C. Petersen, Gerald B. Call, John M. Chaston
    7 Jan 2021
  • Chromosome-Level <span class="named-content genus-species" id="named-content-1">Aspergillus flavus</span> Strain CA14 Genome Assembly
    Genome Sequences
    Chromosome-Level Aspergillus flavus Strain CA14 Genome Assembly

    We report here a chromosome-level genome assembly of the aflatoxigenic fungus Aspergillus flavus strain CA14. This strain is the basis for numerous studies in fungal physiology and secondary metabolism. This full-length assembly will aid in subsequent genomics research.

    Mark A. Weaver, Brian M. Mack, Matthew K. Gilbert
    7 Jan 2021
  • Coding-Complete Genome Sequences of an Iteradensovirus and an Alphapermutotetra-Like Virus Identified from the Pine Processionary Moth (<span class="named-content genus-species" id="named-content-1">Thaumetopoea pityocampa</span>) in Portugal
    Genome Sequences
    Coding-Complete Genome Sequences of an Iteradensovirus and an Alphapermutotetra-Like Virus Identified from the Pine Processionary Moth (Thaumetopoea pityocampa) in Portugal

    The coding-complete nucleotide sequences of an iteradensovirus (family Parvoviridae) and an alphapermutotetra-like virus (family Permutotetraviridae) were discovered from transcriptomic data sets obtained from Thaumetopoea pityocampa larvae collected in Portugal. Each of the coding-complete genome sequences of these viruses contain three main open...

    Franck Dorkeld, Réjane Streiff, Carole Kerdelhué, Mylène Ogliastro
    7 Jan 2021
  • Complete Genome Sequence of Novel Polerovirus-Associated RNA Infecting Pepper (<em>Capsicum</em> spp.) in South Africa
    Genome Sequences
    Complete Genome Sequence of Novel Polerovirus-Associated RNA Infecting Pepper (Capsicum spp.) in South Africa

    The complete genome sequence of novel polerovirus-associated RNA infecting pepper in South Africa was determined. The nucleotide sequence identity of 78.3% with closely related species suggested that this associated RNA was novel, and the name Pepper Vein Yellows Virus-associated RNA is proposed for this RNA fragment.

    W. E. W. Schravesande, J. P. van Wijk, A. Verhage
    7 Jan 2021
  • Genome Sequence of <span class="named-content genus-species" id="named-content-1">Hymenobacter polaris</span> RP-2-7<sup>T</sup>, Isolated from Arctic Soil
    Genome Sequences
    Genome Sequence of Hymenobacter polaris RP-2-7T, Isolated from Arctic Soil

    Hymenobacter polaris RP-2-7T was isolated from soil from the Arctic region. This study presents the genome sequence of Hymenobacter polaris RP-2-7T, generated using the Illumina HiSeq platform. The genome size is 5,587,174 bp; it contains 4,721 genes and has 62.8 mol% DNA G+C content.

    Aravind Sundararaman, Ram Hari Dahal, Dong-Uk Kim, Jaisoo Kim, Jitendra Upadhyaya, Yongseok Hong, Dhiraj Kumar Chaudhary
    7 Jan 2021
  • Closed Genome and Plasmid Sequences of <span class="named-content genus-species" id="named-content-1">Legionella pneumophila</span> AW-13-4, Isolated from a Hot Water Loop System of a Large Occupational Building
    Genome Sequences
    Closed Genome and Plasmid Sequences of Legionella pneumophila AW-13-4, Isolated from a Hot Water Loop System of a Large Occupational Building

    Unused water in unoccupied buildings can become stagnant, with reductions in temperature and levels of disinfectant resulting in increased microbial growth. We report the closed and complete genome and plasmid of Legionella pneumophila strain AW-13-4 (serogroup 1), which was isolated from a hot water loop system of a large building.

    Vicente Gomez-Alvarez, Laura Boczek, Ian Raffenberg, Randy P. Revetta
    7 Jan 2021
  • Draft Genome Sequence of the Termite-Associated “Cuckoo Fungus,” <em>Athelia</em> (<em>Fibularhizoctonia</em>) sp. TMB Strain TB5
    Genome Sequences
    Draft Genome Sequence of the Termite-Associated “Cuckoo Fungus,” Athelia (Fibularhizoctonia) sp. TMB Strain TB5

    Atheliales is a diverse order of crust-forming Basidiomycota fungi. Here, we report the draft genome of the “cuckoo fungus,” Athelia (Fibularhizoctonia) sp. strain TMB (Atheliales), which forms termite-egg-mimicking sclerotia for which termites care. We further compare its repertoire of psilocybin gene homologs to homologs previously reported for Fibularhizoctonia psychrophila.

    Zachary Konkel, Kelsey Scott, Jason C. Slot
    7 Jan 2021
  • Complete Genome Sequence of a Rhabdovirus Strain from <em>Culex</em> Mosquitos Collected in Southern Switzerland
    Genome Sequences
    Complete Genome Sequence of a Rhabdovirus Strain from Culex Mosquitos Collected in Southern Switzerland

    We report here the full-length genome sequence of a rhabdovirus strain detected in a pool of 21 Culex pipiens and Culex torrentium mosquitos collected in southern Switzerland. The genome has a length of 11,914 nucleotides and encodes five major putative open reading frames.

    Jakub Kubacki, Isabelle Hardmeier, Weihong Qi, Eleonora Flacio, Mauro Tonolla, Cornel Fraefel
    7 Jan 2021
  • Complete Genome Sequence of Nisin-Producing <span class="named-content genus-species" id="named-content-1">Lactococcus lactis</span> subsp. <em>lactis</em> N8
    Genome Sequences
    Complete Genome Sequence of Nisin-Producing Lactococcus lactis subsp. lactis N8

    We report here the genome sequence of Lactococcus lactis N8, a nisin producer isolated in the 1960s from a dairy product in Finland. The genome consists of a 2.42-Mb chromosome and two plasmids of 80.3 and 71.3 kb.

    Xing Wan, Timo M. Takala, Mingqiang Qiao, Per E. J. Saris
    7 Jan 2021
  • Complete Genome Sequences of Three <span class="named-content genus-species" id="named-content-1">Listeria monocytogenes</span> Bacteriophage Propagation Strains
    Genome Sequences
    Complete Genome Sequences of Three Listeria monocytogenes Bacteriophage Propagation Strains

    Bacteriophages can be used as a biocontrol for the foodborne pathogen Listeria monocytogenes. Propagation of phages is a necessary step for their use in experimental studies and biocontrol applications. Here, we present the complete genomes of three Listeria monocytogenes strains commonly used as...

    Lauren K. Hudson, Tracey Lee Peters, Daniel W. Bryan, Yaxiong Song, Henk C. den Bakker, Thomas G. Denes
    7 Jan 2021
  • A 24-Year-Old Sample Contributes the Complete Genome Sequence of Fowl Aviadenovirus D from the United States
    Genome Sequences
    A 24-Year-Old Sample Contributes the Complete Genome Sequence of Fowl Aviadenovirus D from the United States

    Here, we report the complete genome sequence of fowl adenovirus D (FAdV-D) isolated from a preserved 24-year-old pancreas sample of a broiler chicken embryo. The results of the sequence showed that the viral genome is 44,079 bp long.

    Iryna V. Goraichuk, James F. Davis, Arun B. Kulkarni, Claudio L. Afonso, David L. Suarez
    7 Jan 2021
  • Draft Genome Sequence of <em>Streptomyces</em> sp. Isolate H28 from the Meycauayan River, Philippines
    Genome Sequences
    Draft Genome Sequence of Streptomyces sp. Isolate H28 from the Meycauayan River, Philippines

    In this paper, we report the draft genome sequence of Streptomyces sp. isolate H28, isolated from the sediments of Meycauayan River, Philippines. This species exhibits production of melanin as well as the ability to utilize and degrade both high-density polyethylene (HDPE) and low-density polyethylene (LDPE).

    Aroin B. Verdera, Andrew D. Montecillo, Marie Christine M. Obusan
    7 Jan 2021
  • Complete Genome Sequence of <em>Streptomyces</em> Siphophage Sitrop
    Genome Sequences
    Complete Genome Sequence of Streptomyces Siphophage Sitrop

    Streptomyces sp. strain Mg1 is a Gram-positive soil bacterium capable of causing cell lysis and degradation of Bacillus subtilis colonies. Here, we report the 48,481-bp genome of Streptomyces sp. Mg1 siphophage Sitrop. With 77 predicted protein-coding genes and one tRNA, Sitrop shares 77% nucleotide sequence identity with the Streptomyces...

    Victor Portillo, Haydee Diaz, James Clark, Isla Hernandez, Mei Liu, Ben Burrowes
    7 Jan 2021
  • Complete Genome Sequences of Two <span class="named-content genus-species" id="named-content-1">Mycobacterium smegmatis</span> Bacteriophages
    Genome Sequences
    Complete Genome Sequences of Two Mycobacterium smegmatis Bacteriophages

    Mycobacterium smegmatis, an acid-fast bacterial species in the phylum Actinobacteria, has often been used as a substitute for pathogenic mycobacteria in research. Here, we describe the isolation and characterization of two M. smegmatis bacteriophages, Penelope2018 and Miniwave.

    ...
    Darlenys Sanchez, Anna Acosta, Nicole Skoumpourdis, Dominican College SEA-PHAGES Annotators, Regina Alvarez, Bernadette J. Connors
    7 Jan 2021
  • Draft Genome Sequences of 19 Clinical Isolates of <span class="named-content genus-species" id="named-content-1">Candida auris</span> from Hong Kong
    Genome Sequences
    Draft Genome Sequences of 19 Clinical Isolates of Candida auris from Hong Kong

    Candida auris is an emerging human pathogen associated with multidrug resistance and nosocomial outbreaks. We report the draft genome sequences of 19 C. auris isolates that were associated with a cluster of cases in a hospital in Hong Kong.

    Herman Tse, Alan K. L. Tsang, Yiu-Wai Chu, Dominic N. C. Tsang
    7 Jan 2021
  • Genome Sequence of <span class="named-content genus-species" id="named-content-1">Bifidobacterium breve</span> INIA P734 (CECT 8178), a Strain Isolated from Human Breast Milk
    Genome Sequences
    Genome Sequence of Bifidobacterium breve INIA P734 (CECT 8178), a Strain Isolated from Human Breast Milk

    The draft genome sequence of Bifidobacterium breve INIA P734, a strain shared by mother and child, is reported. It consists of 50 contigs, with 2,391,925 bp, 2,099 genes, and a G+C content of 58.8%. The genome analysis revealed the absence of antibiotic resistance and pathogenicity-related genes.

    Lidia Rodrigo-Torres, Eva Rodríguez, Ángela Peirotén, Susana Langa, Margarita Medina, David R. Arahal, Rosa Aznar, Juan L. Arqués
    7 Jan 2021
  • Complete Genome Sequence of a Serotype 7 <span class="named-content genus-species" id="named-content-1">Listeria monocytogenes</span> Strain, FSL R9-0915
    Genome Sequences
    Complete Genome Sequence of a Serotype 7 Listeria monocytogenes Strain, FSL R9-0915

    Listeria monocytogenes serotype 7 lacks glycosidic constituents in wall teichoic acids. Here, we present the complete genome sequence of L. monocytogenes serotype 7 strain FSL R9-0915 and an analysis of genes known to affect L....

    Tracey Lee Peters, Lauren K. Hudson, Daniel W. Bryan, Yaxiong Song, Henk C. den Bakker, Zuzana Kucerova, Thomas G. Denes
    7 Jan 2021
  • Draft Genome Sequence of <span class="named-content genus-species" id="named-content-1">Salmonella enterica</span> subsp. <em>enterica</em> Serovar Livingstone 1236H, a Desiccation-Resistant Strain That Poses a Salmonellosis Hazard in Low-Moisture Foods
    Genome Sequences
    Draft Genome Sequence of Salmonella enterica subsp. enterica Serovar Livingstone 1236H, a Desiccation-Resistant Strain That Poses a Salmonellosis Hazard in Low-Moisture Foods

    Salmonella enterica serovar Livingstone 1236H was isolated originally from peanut butter and represents a health risk in low-moisture foods. The current work presents the strain’s genome sequencing results, which show a 4,824,729-bp genome sequence and 4,435 protein coding sequences, including some that are involved in adaptation to low-moisture environments.

    ...
    Ahmed G. Abdelhamid, Yumin Xu, Ahmed E. Yousef
    7 Jan 2021
  • Complete Genome Sequence of Bisphenol A-Degrading Bacterium <em>Sphingobium</em> sp. Strain A3, Isolated from Contaminated Soil
    Genome Sequences
    Complete Genome Sequence of Bisphenol A-Degrading Bacterium Sphingobium sp. Strain A3, Isolated from Contaminated Soil

    This study reports the complete genome sequence of bisphenol A-degrading bacterium Sphingobium sp. strain A3, which was isolated from a contaminated soil sample from the site of a factory fire in South Korea. The genome consists of a 6.53-Mbp chromosome and eight plasmid contigs (532,947 bp), with 6,406 protein-coding sequences and a GC content of 63.82%.

    Ji Young Jung, Hye Kyeoung Kang, Dae Won Jeong, Hyun Mi Jin, Byung-Gon Ryu, Bok Yeon Jo, Eu Jin Chung, Sang-Soo Han
    7 Jan 2021
  • Draft Genome Sequences of Four <em>Citrobacter</em> Isolates Recovered from Wild Australian Shorebirds
    Genome Sequences
    Draft Genome Sequences of Four Citrobacter Isolates Recovered from Wild Australian Shorebirds

    Citrobacter is a ubiquitous bacterial genus whose members inhabit a variety of niches. Some species are clinically important for both antimicrobial resistance (AMR) carriage and as the cause of nosocomial infections. Surveillance of Citrobacter species in the environment can provide indicators of the spread of AMR genes outside clinical spaces. In this study, we present draft genome sequences of four Citrobacter...

    Hannah G. P. Smith, David C. Bean, William Pitchers, Mary Valcanis, Rohan H. Clarke, Richard Loyn, Chris J. Hassell, Andrew R. Greenhill
    7 Jan 2021
  • Draft Genome Sequence of <span class="named-content genus-species" id="named-content-1">Pseudomonas saudiphocaensis</span> Strain AGROB56, Isolated from a Dairy Farm in New Zealand
    Genome Sequences
    Draft Genome Sequence of Pseudomonas saudiphocaensis Strain AGROB56, Isolated from a Dairy Farm in New Zealand

    Here, we report the draft genome sequence of a new Pseudomonas saudiphocaensis strain, AGROB56, with lipolytic potential, isolated from a sheep dairy farm in New Zealand. The genome is 3.61 Mbp, with a GC content of 61.1%, and the genome sequence was found closely related to Pseudomonas saudiphocaensis 20 BNT

    Tanushree B. Gupta, Alexis N. Risson, Ruy Jauregui, Paul Maclean, Gale Brightwell
    7 Jan 2021
  • Draft Genome Sequences of 284 <span class="named-content genus-species" id="named-content-1">Xanthomonas citri</span> pv. citri Strains Causing Asiatic Citrus Canker
    Genome Sequences
    Draft Genome Sequences of 284 Xanthomonas citri pv. citri Strains Causing Asiatic Citrus Canker

    High-quality Illumina assemblies were produced from 284 Xanthomonas citri pv. citri pathotype A strains mostly originating from the Southwest Indian Ocean region, a subset of which was also sequenced using MinION technology. Some strains hosted chromosomally encoded transcription activator-like effector (TALE) genes, an atypical feature for this bacterium.

    ...
    Damien Richard, Adrien Rieux, Pierre Lefeuvre, Azali Hamza, Kanta Kumar Lobin, Mark Naiken, Randy Stravens, Claudine Boyer, Karine Boyer, Stéphanie Javegny, Olivier Pruvost
    7 Jan 2021
  • Complete Genome Sequences of Four Atrazine-Degrading Bacterial Strains, <em>Pseudomonas</em> sp. Strain ADPe, <em>Arthrobacter</em> sp. Strain TES, <em>Variovorax</em> sp. Strain 38R, and <em>Chelatobacter</em> sp. Strain SR38
    Genome Sequences
    Complete Genome Sequences of Four Atrazine-Degrading Bacterial Strains, Pseudomonas sp. Strain ADPe, Arthrobacter sp. Strain TES, Variovorax sp. Strain 38R, and Chelatobacter sp. Strain SR38

    We report here the complete genome sequences of four atrazine-degrading bacteria. Their genomes will serve as references for determining the genetic changes that have occurred during an evolution experiment.

    Loren Billet, Marion Devers-Lamrani, Rémy-Félix Serre, Emmanuel Julia, Céline Vandecasteele, Nadine Rouard, Fabrice Martin-Laurent, Aymé Spor
    7 Jan 2021
  • Complete Genome Sequence of <span class="named-content genus-species" id="named-content-1">Clostridium botulinum</span> CJ0611A1, a Type A(B) Isolate Associated with an International Outbreak of Botulism from Commercial Carrot Juice
    Genome Sequences
    Complete Genome Sequence of Clostridium botulinum CJ0611A1, a Type A(B) Isolate Associated with an International Outbreak of Botulism from Commercial Carrot Juice

    We report the complete genome (3.9-Mb chromosome, 5.9-kb plasmid) of Clostridium botulinum CJ0611A1, a type A(B) strain isolated from carrot juice distributed in Canada and linked to an international 2006 foodborne botulism outbreak. This strain encodes a full-length bont/A1 gene and a truncated bont/B gene.

    Richard Harris, Forest Dussault, Annika Flint, John W. Austin, Kelly Weedmark
    7 Jan 2021
  • Complete Genome Sequence of <span class="named-content genus-species" id="named-content-1">Sphingobium barthaii</span> KK22, a High-Molecular-Weight Polycyclic Aromatic Hydrocarbon-Degrading Soil Bacterium
    Genome Sequences
    Complete Genome Sequence of Sphingobium barthaii KK22, a High-Molecular-Weight Polycyclic Aromatic Hydrocarbon-Degrading Soil Bacterium

    Sphingobium barthaii KK22T is a high-molecular-weight polycyclic aromatic hydrocarbon-degrading soil bacterium that has been investigated in biotransformation, microbial ecology, and DNA damage studies. The complete genome sequence of S. barthaii revealed four closed circular sequences,...

    Jiro F. Mori, Robert A. Kanaly
    7 Jan 2021
  • Draft Genome Sequences of Eight <span class="named-content genus-species" id="named-content-1">Campylobacter volucris</span> Isolates from Freshwater Sources in Victoria, Australia
    Genome Sequences
    Draft Genome Sequences of Eight Campylobacter volucris Isolates from Freshwater Sources in Victoria, Australia

    Campylobacter spp. can survive and be transmitted from a range of environments. Here, we examine eight draft genome sequences of Campylobacter volucris, identified as part of an examination of waterborne Campylobacter species. This is the first report of environmental survival of C. volucris outside gull species.

    Agnus M. Davis, David. T. McCarthy, Dieter M. Bulach, Rebekah Henry
    7 Jan 2021
  • Near-Complete Sequence of a Highly Divergent Reovirus Genome Recovered from <span class="named-content genus-species" id="named-content-1">Callinectes sapidus</span>
    Genome Sequences
    Near-Complete Sequence of a Highly Divergent Reovirus Genome Recovered from Callinectes sapidus

    This report describes the sequence of a reovirus genome, discovered in Callinectes sapidus in Brazil. The genome sequence of CsRV2 consists of 12 segments that encode 13 putative proteins. The predicted RNA-dependent RNA polymerase is highly similar to that of Eriocheir sinensis reovirus 905,...

    Mingli Zhao, Emily M. Flowers, Eric J. Schott
    7 Jan 2021
  • Draft Genome Sequence of Medusavirus Stheno, Isolated from the Tatakai River of Uji, Japan
    Genome Sequences
    Draft Genome Sequence of Medusavirus Stheno, Isolated from the Tatakai River of Uji, Japan

    “Medusaviridae” is a proposed family of large double-stranded DNA (dsDNA) viruses so far represented by a sole virus isolated from a hot spring. In the present study, we report the isolation and genome sequencing of a second member of this family, Medusavirus stheno, discovered from a freshwater sample with an...

    Koki Yoshida, Ruixuan Zhang, Kimberly G. Garcia, Hisashi Endo, Yasuhiro Gotoh, Tetsuya Hayashi, Masaharu Takemura, Hiroyuki Ogata
    7 Jan 2021
  • Genome Annotations of Two <em>Bacillus</em> Phages, Tomato and BaseballField
    Genome Sequences
    Genome Annotations of Two Bacillus Phages, Tomato and BaseballField

    Tomato and Baseball Field are Bacillus bacteriophages that were isolated and annotated by students in the Howard Hughes Medical Institute Phage Hunters program. Tomato has a unique truncation of the tape measure gene that is not found in other closely related C1 Bacillus phages. Baseball Field is a strictly lytic phage with a compact genome of 26 kb.

    Julie Duperier, Maikah Bulpitt, Felipe Bispo, Elizabeth Greguske
    7 Jan 2021
  • Coding-Complete Genome Sequence of a Yellow Fever Virus Isolated from a Baby Howler Monkey (<em>Alouatta caraya</em>) from São Paulo State, Brazil, in 2016
    Genome Sequences
    Coding-Complete Genome Sequence of a Yellow Fever Virus Isolated from a Baby Howler Monkey (Alouatta caraya) from São Paulo State, Brazil, in 2016

    We report a coding-complete sequence of a yellow fever virus, strain JabSPM02, containing the 3′ untranslated region and all coding regions. The virus was recovered from an infected howler monkey from a rural area in São Paulo State, Brazil. Our findings show that it belongs to the South America 1E genotype.

    Márcio Junio Lima Siconelli, Daniel Macedo de Melo Jorge, Luiza Antunes de Castro-Jorge, Benedito Antonio Lopes da Fonseca
    7 Jan 2021
  • Complete Genome Sequences of Novel Bovine T4, rv5-Like, and Dhillonviruses Effective against <span class="named-content genus-species" id="named-content-1">Escherichia coli</span> O157
    Genome Sequences
    Complete Genome Sequences of Novel Bovine T4, rv5-Like, and Dhillonviruses Effective against Escherichia coli O157

    Enterohemorrhagic Escherichia coli (EHEC) isolates of serotype O157:H7 are serious foodborne zoonotic pathogens and prime targets for biocontrol using bacteriophages. We report on the complete genome sequences of 11 novel lytic bacteriophages, representing three viral genera, isolated from cattle in Hungary that target...

    Domonkos Sváb, Linda Falgenhauer, Trinad Chakraborty, István Tóth
    7 Jan 2021
  • Draft Genome Sequence of the Phosphate-Solubilizing Rhizobacterium <span class="named-content genus-species" id="named-content-1">Burkholderia pseudomultivorans</span> Strain MPSB1, Isolated from a Copper Mined-Out Site
    Genome Sequences
    Draft Genome Sequence of the Phosphate-Solubilizing Rhizobacterium Burkholderia pseudomultivorans Strain MPSB1, Isolated from a Copper Mined-Out Site

    Burkholderia pseudomultivorans MPSB1 was isolated from a copper mined-out soil sample collected from Mogpog, Marinduque, Philippines. Here, we report the draft genome sequence with predicted gene inventories supporting rhizosphere bioremediation, such as heavy metal tolerance, phosphate solubilization, and siderophore production.

    Berna Lou L. Aba-Regis, Kristel Mae P. Oliveros, Cherry Ibarra-Romero, Asuncion K. Raymundo, Nelly S. Aggangan, Teofila O. Zulaybar, Albert Remus R. Rosana
    7 Jan 2021
  • Complete Genome Sequence of <em>Streptomyces</em> Phage Shady
    Genome Sequences
    Complete Genome Sequence of Streptomyces Phage Shady

    Streptomyces spp. are saprophytic soil bacteria that produce secondary metabolites with therapeutic potential. This announcement describes the isolation and genome annotation of Streptomyces sp. strain Mg1 siphophage Shady. Learning more about Shady’s novel 45-kb genome, containing 76 predicted protein-coding genes, could be industrially advantageous when using streptomycetes for their products.

    Carlos Ortega, Isla Hernandez, James Clark, Mei Liu, Ben Burrowes
    7 Jan 2021
  • Complete Genome Sequence of <em>Streptomyces</em> Phage Shaeky
    Genome Sequences
    Complete Genome Sequence of Streptomyces Phage Shaeky

    Here, we present the genome of siphophage Shaeky, infecting the Gram-positive bacterium Streptomyces sp. strain Mg1. Shaeky has very low sequence identity to other phages, with phage phiC31 being the most closely related in the NCBI database. The Shaeky genome is 45,617 bp with 77 protein-coding genes and 16 tRNAs.

    Shae Shodrock, Tyler Higbee, James Clark, Isla Hernandez, Mei Liu, Ben Burrowes
    7 Jan 2021
  • Complete Genome Sequence of <em>Streptomyces</em> Siphophage Sycamore
    Genome Sequences
    Complete Genome Sequence of Streptomyces Siphophage Sycamore

    Streptomyces sp. strain Mg1 is a competitive soil-dwelling bacterium that secretes antibiotics that inhibit growth of Bacillus subtilis. Here, we present the genome sequence of Sycamore, a 44,694-bp Streptomyces sp. Mg1 siphophage with 66 predicted protein-coding genes, that is similar to phage genome sequences in the Lomovskayavirus genus...

    Xing-Han Zhang, Alejandra Marquez, James Clark, Isla Hernandez, Madeline Rivera, Mei Liu, Ben Burrowes
    7 Jan 2021
  • Mutation Profile of SARS-CoV-2 Genome Sequences Originating from Eight Israeli Patient Isolates
    Genome Sequences
    Mutation Profile of SARS-CoV-2 Genome Sequences Originating from Eight Israeli Patient Isolates

    We report the genome sequences and the identification of genetic variations in eight clinical samples of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Samples were collected from nasopharyngeal swabs of symptomatic and asymptomatic individuals from five care homes for elderly and infirm persons in Israel. The sequences obtained are valuable, as they carry a newly reported nonsynonymous substitution located within the...

    Galia Zaide, Inbar Cohen-Gihon, Ofir Israeli, Dana Stein, Ohad Shifman, Shay Weiss, Irit Simon, Orly Laskar, Adi Beth-Din, Anat Zvi
    7 Jan 2021
  • Genomic Sequences and Analysis of Five SARS-CoV-2 Variants Obtained from Patients in Lambayeque, Peru
    Genome Sequences
    Genomic Sequences and Analysis of Five SARS-CoV-2 Variants Obtained from Patients in Lambayeque, Peru

    Here, we report the genomic sequences of five severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strains obtained from nasopharyngeal samples from five tested COVID-19-infected patients from the Lambayeque region in Peru during early April 2020.

    Franklin Rómulo Aguilar-Gamboa, Luis Alberto Salcedo-Mejía, Luis Miguel Serquén-López, Marco Enrique Mechan-Llontop, Percy Omar Tullume-Vergara, Juan José Bonifacio-Briceño, Ramsés Salas-Asencios, Heber Silva Díaz, Juan P. Cárdenas
    7 Jan 2021
  • Complete Genome Sequence of <span class="named-content genus-species" id="named-content-1">Paludibaculum fermentans</span> P105<sup>T</sup>, a Facultatively Anaerobic Acidobacterium Capable of Dissimilatory Fe(III) Reduction
    Genome Sequences
    Complete Genome Sequence of Paludibaculum fermentans P105T, a Facultatively Anaerobic Acidobacterium Capable of Dissimilatory Fe(III) Reduction

    Paludibaculum fermentans P105T is a facultatively anaerobic heterotroph of the phylum Acidobacteria which is capable of dissimilatory Fe(III) reduction. This bacterium is a common inhabitant of wetlands and groundwater bodies. The finished genome of strain P105T is 9.53 Mb in size and contains multiple genes coding for membrane-bound...

    Svetlana N. Dedysh, Alexey V. Beletsky, Irina S. Kulichevskaya, Andrey V. Mardanov, Nikolai V. Ravin
    7 Jan 2021
  • Complete Genome Sequence of <em>Streptomyces</em> Phage Sentinel
    Genome Sequences
    Complete Genome Sequence of Streptomyces Phage Sentinel

    The Streptomyces genus produces over two-thirds of clinically useful, natural antibiotics. Here, we describe the isolation and genome annotation of siphophage Sentinel, which utilizes Streptomyces sp. strain Mg1 as a host. It has a 50,272-bp genome and 83 protein-coding genes and shows similarity to other Streptomyces phages in the Arequatrovirus genus.

    Andrew Talcott, Russell Moreland, Jolene Ramsey, James Clark, Isla Hernandez, Mei Liu, Ben Burrowes
    7 Jan 2021
  • Genome Sequences of Two GH Clade SARS-CoV-2 Strains Isolated from Patients with COVID-19 in South Korea
    Genome Sequences
    Genome Sequences of Two GH Clade SARS-CoV-2 Strains Isolated from Patients with COVID-19 in South Korea

    We report the genome sequences of two GH clade severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strains isolated from nasopharyngeal swabs from patients with coronavirus disease 2019 (COVID-19) in South Korea. These strains had two mutations in the untranslated regions and seven nonsynonymous substitutions in open reading frames, compared with Wuhan/Hu-1/2019, showing 99.96% sequence identity.

    Minwoo Kim, Youn-Jung Lee, Jae Sun Yoon, Jin Young Ahn, Jung Ho Kim, Jun Yong Choi, Jong-Won Oh
    7 Jan 2021
  • Draft Genome Sequence and Secondary Metabolite Biosynthetic Potential of the <span class="named-content genus-species" id="named-content-1">Lysobacter niastensis</span> Type Strain DSM 18481
    Genome Sequences
    Draft Genome Sequence and Secondary Metabolite Biosynthetic Potential of the Lysobacter niastensis Type Strain DSM 18481

    Lysobacter niastensis belongs to a group of bacterial predators that produce a number of bioactive small molecules endowed with lytic properties toward other microorganisms. Here, we report the draft genome sequence of the type strain DSM 18481 and the identification of gene clusters implicated in the biosynthesis of secondary metabolites.

    Paolo Turrini, Irene Artuso, Marco Tescari, Gabriele Andrea Lugli, Emanuela Frangipani, Marco Ventura, Paolo Visca
    7 Jan 2021
  • Complete Genome Sequence of <em>Streptomyces</em> Phage Spernnie
    Genome Sequences
    Complete Genome Sequence of Streptomyces Phage Spernnie

    Streptomyces spp. are Gram-positive soil bacteria that have been reported in some cases to cause acute and chronic infections, including mycetomas, pneumonia, and septicemia. Here, we present Streptomyces sp. strain Mg1 phage Spernnie. Spernnie is a temperate siphophage containing 89 predicted coding genes in a 50,834-bp genome sequence.

    Nathaniel Tate, Loraine Melbern, James Clark, Isla Hernandez, Mei Liu, Ben Burrowes
    7 Jan 2021
  • Complete Genome Sequence of <em>Sandaracinobacter</em> sp. Strain M6, Isolated from a Rocky Mountain in China
    Genome Sequences
    Complete Genome Sequence of Sandaracinobacter sp. Strain M6, Isolated from a Rocky Mountain in China

    We report the complete genome sequence of Sandaracinobacter sp. strain M6 obtained by Oxford Nanopore and Illumina sequencing. The approximately 3.4 Mb genome sequence with a GC content of 67.65% provides essential data for future taxonomic studies and information for further investigating the metabolic characteristics of aerobic anoxygenic phototrophs.

    Ying Tang, Jun Huang, Cuiyang Zhang, Shiyu Bi, Zhaohui Guo, Qingshu Liu, Ping Lei
    7 Jan 2021
  • Draft Genome Sequence of the Free-Living, Iridescent Bacterium <span class="named-content genus-species" id="named-content-1">Tenacibaculum mesophilum</span> Strain ECR
    Genome Sequences
    Draft Genome Sequence of the Free-Living, Iridescent Bacterium Tenacibaculum mesophilum Strain ECR

    Here, we report the genome sequence of Tenacibaculum mesophilum strain ECR, which was isolated from the river/ocean interface at Trunk River, Falmouth, Massachusetts, USA. The isolation and sequencing were performed as part of the 2016 and 2018 Microbial Diversity courses at the Marine Biological Laboratory in Woods Hole, Massachusetts, USA.

    Rebecca L. Mickol, Artemis S. Louyakis, H. Lynn Kee, Lisa K. Johnson, Scott C. Dawson, Katherine R. Hargreaves, Grayson L. Chadwick, Dianne K. Newman, Jared R. Leadbetter, C. Titus Brown
    7 Jan 2021
  • Draft Genome Sequence of Plant Growth-Promoting <span class="named-content genus-species" id="named-content-1">Bacillus velezensis</span> BS89
    Genome Sequences
    Draft Genome Sequence of Plant Growth-Promoting Bacillus velezensis BS89

    The plant growth-promoting bacterium Bacillus velezensis BS89 was isolated from the rhizosphere of winter wheat. Strain BS89 has the ability to promote plant growth and produce a mix of auxins and vitamins. Here, we sequenced the complete genome of this strain to understand the molecular mechanisms underlying its beneficial activities.

    Vladimir K. Chebotar, Gerben P. Voshol, Natalia V. Malfanova, Elena P. Chizhevskaya, Alexander N. Zaplatkin, Oksana V. Komarova, Maria E. Baganova, Alexander M. Lazarev, Svetlana V. Balakina
    7 Jan 2021

Omics Data Sets

  • Survey of Maize Rhizosphere Microbiome Using Shotgun Metagenomics
    Omics Data Sets
    Survey of Maize Rhizosphere Microbiome Using Shotgun Metagenomics

    Several processes which occur in the rhizosphere make it a vital region in plant development. However, studies that examine the rhizosphere microbiome and their functional potentials remain scarce. Shotgun metagenomics was employed here to evaluate the functional potentials of the maize rhizosphere microbiome of farms in two South African provinces.

    Olubukola Oluranti Babalola, Oluwadara Pelumi Omotayo, Nicholas Ozede Igiehon
    7 Jan 2021
  • Metagenomic Exploration of <em>Atelerix albiventris</em> Gut Microbiome
    Omics Data Sets
    Metagenomic Exploration of Atelerix albiventris Gut Microbiome

    Here, we report the metagenomic analysis of the gut of Atelerix albiventris, an animal typically kept as a pet in Kazakhstan. In this case, shotgun metagenomic sequencing of the RNA and DNA viral community was performed.

    Makhabbat Amanbayeva, Elmira Anarkulova, Andrey Bogoyavlenskiy, Madina Alexyuk, Anar Imangazy, Vladimir Berezin
    7 Jan 2021

Amplicon Sequence Collections

  • Bacterial Diversity Profiling around the Orca Seamount in the Bransfield Strait, Antarctica, Based on 16S rRNA Gene Amplicon Sequences
    Amplicon Sequence Collections
    Bacterial Diversity Profiling around the Orca Seamount in the Bransfield Strait, Antarctica, Based on 16S rRNA Gene Amplicon Sequences

    Hydrothermal vent activity is often associated with submarine volcanism. Here, we investigated the presence of microorganisms related to hydrothermal activity in the Orca seamount. Data profiling of the 16S rRNA gene amplicon sequences revealed a diversity pattern dominated mainly by the phyla Proteobacteria, Acidobacteria, Planctomycetes, and Bacteroidetes.

    Wilbert Serrano, Raul M. Olaechea, Luis Cerpa, Jose Herrera, Aldo Indacochea
    7 Jan 2021
  • 16S rRNA Gene Amplicon Sequencing of Gut Microbiota in Three Species of Deep-Sea Fish in Suruga Bay, Japan
    Amplicon Sequence Collections
    16S rRNA Gene Amplicon Sequencing of Gut Microbiota in Three Species of Deep-Sea Fish in Suruga Bay, Japan

    We report here 16S rRNA gene amplicon sequence analysis of the gut microbiota in three species of deep-sea fish collected from Suruga Bay, Japan. Of the three species, two were dominated by the phylum Proteobacteria (genus Photobacterium), while one was dominated by the phyla Spirochaetes (genus Brevinema) and Tenericutes (unclassified Mycoplasmataceae).

    Toshihide Iwatsuki, Takahiro Kanazawa, Takato Ogasawara, Kento Hosotani, Karen Tsuchiya, Shinichi Watanabe, Tomoko Suzuki, Ryota Moriuchi, Yu Kanesaki, Hideo Dohra
    7 Jan 2021

Culture Collections/Mutant Libraries

  • Characterization of <span class="named-content genus-species" id="named-content-1">Clostridioides difficile</span> Isolates Available through the CDC &amp; FDA Antibiotic Resistance Isolate Bank
    Culture Collections/Mutant Libraries
    Characterization of Clostridioides difficile Isolates Available through the CDC & FDA Antibiotic Resistance Isolate Bank

    Thirty Clostridioides difficile isolates collected in 2016 through the Centers for Disease Control and Prevention Emerging Infections Program were selected for reference antimicrobial susceptibility testing and whole-genome sequencing. Here, we present the genetic characteristics of these isolates and announce their availability in the CDC & FDA Antibiotic...

    Ashley Paulick, Michelle Adamczyk, Karen Anderson, Nick Vlachos, María-José Machado, Gillian McAllister, Lauren Korhonen, Alice Y. Guh, Alison Laufer Halpin, J. Kamile Rasheed, Maria Karlsson, Joseph D. Lutgring, Amy S. Gargis, the Emerging Infections Program Clostridioides difficile Pathogen Group
    7 Jan 2021
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Microbiology Resource Announcements: 10 (1)

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volume 10, issue 1
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