Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Microbiology Resource Announcements
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Latest Articles
    • Archive
  • Types of Resources
    • Amplicon Sequence Collections
    • Culture Collections/Mutant Libraries
    • Databases and Software
    • Omics Data Sets
    • Other Genetic Resources
    • Genome Sequences
  • For Authors
    • Getting Started
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About MRA
    • Editor in Chief
    • Board of Editors
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Microbiology Resource Announcements
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Microbiology Resource Announcements
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Latest Articles
    • Archive
  • Types of Resources
    • Amplicon Sequence Collections
    • Culture Collections/Mutant Libraries
    • Databases and Software
    • Omics Data Sets
    • Other Genetic Resources
    • Genome Sequences
  • For Authors
    • Getting Started
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About MRA
    • Editor in Chief
    • Board of Editors
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
Prokaryotes

Complete Genome Sequences of Azotobacter vinelandii Wild-Type Strain CA and Tungsten-Tolerant Mutant Strain CA6

Jesse D. Noar, José M. Bruno-Bárcena
Jesse D. Noar
North Carolina State University, Raleigh, North Carolina, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
José M. Bruno-Bárcena
North Carolina State University, Raleigh, North Carolina, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/genomeA.00313-13
  • Article
  • Info & Metrics
  • PDF
Loading

ABSTRACT

We report the complete genome sequences of Azotobacter vinelandii mutant strain CA6 and its parent wild-type strain, CA. When fixing nitrogen, strain CA6 displays slow growth and impaired molybdate uptake, tolerance to tungstates, and production of hydrogen gas, compared to results for strain CA. Comparing these genome sequences may provide a genetic basis for these mutant phenotypes.

GENOME ANNOUNCEMENT

Azotobacter vinelandii is a Gram-negative, soil-dwelling, obligately aerobic diazotroph that was discovered more than a century ago (1). A. vinelandii strain CA (or OP) (ATCC 13705) was isolated as a nongummy pigment-producing wild-type strain that is easier to study than “gummy” polysaccharide-producing strains (2). A consortium of researchers has published the complete genome sequence of a variant of strain CA, a high-frequency transforming strain called DJ (ATCC BAA-1303) (3).

A. vinelandii strain CA6 is a spontaneous mutant strain derived from strain CA. Tungstate prevents nitrogen fixation and growth of the strain CA, but it does not inhibit CA6 (4). Strain CA6 also displays impaired molybdate uptake (5) and has been found to produce large quantities of hydrogen gas when fixing nitrogen (P. Bishop, T. Loveless, J. Olson, and J. M. Bruno-Bárcena, unpublished data). For this reason, we sequenced the genomes of both CA6 and its parent, CA, to identify the genetic bases of these mutant phenotypes.

The Microbiome Core Facility at the University of North Carolina—Chapel Hill generated shotgun sequence data from each strain using a combination of 454 GS FLX Titanium+ and Ion Torrent PGM techniques, performed according to the manufacturers' instructions. These generated 1,070,910 reads for CA, averaging 416 bp per read, and 1,020,357 reads for CA6, averaging 414 bp per read. Reads were assembled with Geneious (Biomatters, New Zealand) using the genome sequence of strain DJ as a reference (accession no. NC_012560.1). PCR followed by dye terminator sequencing (Eton BioScience, Research Triangle Park, NC) was used to close gaps and confirm or reject uncertain variations identified in silico. Annotations were copied from the GenBank record of the genome of strain DJ and were modified as necessary.

Nucleotide sequence accession numbers.The genome sequences of A. vinelandii strains CA and CA6 have been deposited in DDBJ/EMBL/GenBank under the accession no. CP005094 (CA) and CP005095 (CA6). The versions described in this paper are the first versions, accession no. CP005094 (CA) and CP005095 (CA6).

ACKNOWLEDGMENTS

This project was supported by the North Carolina State University Department of Microbiology and by UNC Microbiome Core Facility grant no. NIH P30 DK34987. J.D.N. is the recipient of a National Science Foundation graduate research fellowship.

FOOTNOTES

    • Received 18 April 2013.
    • Accepted 26 April 2013.
    • Published 6 June 2013.
  • Copyright © 2013 Noar and Bruno-Bárcena.

This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.

REFERENCES

  1. 1.↵
    1. Lipman JG
    . 1903. Experiments on the transformation and fixation of nitrogen by bacteria. New Jersey state agricultural experiment station, 17th annual report. 24:215–285.
    OpenUrl
  2. 2.↵
    1. Bush JA,
    2. Wilson PW
    . 1959. A non-gummy chromogenic strain of Azotobacter vinelandii. Nature 184:381.
    OpenUrlCrossRefPubMed
  3. 3.↵
    1. Setubal JC,
    2. dos Santos P,
    3. Goldman BS,
    4. Ertesvåg H,
    5. Espin G,
    6. Rubio LM,
    7. Valla S,
    8. Almeida NF,
    9. Balasubramanian D,
    10. Cromes L,
    11. Curatti L,
    12. Du Z,
    13. Godsy E,
    14. Goodner B,
    15. Hellner-Burris K,
    16. Hernandez JA,
    17. Houmiel K,
    18. Imperial J,
    19. Kennedy C,
    20. Larson TJ,
    21. Latreille P,
    22. Ligon LS,
    23. Lu J,
    24. Maerk M,
    25. Miller NM,
    26. Norton S,
    27. O'Carroll IP,
    28. Paulsen I,
    29. Raulfs EC,
    30. Roemer R,
    31. Rosser J,
    32. Segura D,
    33. Slater S,
    34. Stricklin SL,
    35. Studholme DJ,
    36. Sun J,
    37. Viana CJ,
    38. Wallin E,
    39. Wang B,
    40. Wheeler C,
    41. Zhu H,
    42. Dean DR,
    43. Dixon R,
    44. Wood D
    . 2009. Genome sequence of Azotobacter vinelandii, an obligate aerobe specialized to support diverse anaerobic metabolic processes. J. Bacteriol. 191:4534–4545.
    OpenUrlAbstract/FREE Full Text
  4. 4.↵
    1. Bishop PE,
    2. Jarlenski DM,
    3. Hetherington DR
    . 1980. Evidence for an alternative nitrogen fixation system in Azotobacter vinelandii. Proc. Natl. Acad. Sci. U. S. A. 77:7342–7346.
    OpenUrlAbstract/FREE Full Text
  5. 5.↵
    1. Premakumar R,
    2. Jacobitz S,
    3. Ricke SC,
    4. Bishop PE
    . 1996. Phenotypic characterization of a tungsten-tolerant mutant of Azotobacter vinelandii. J. Bacteriol. 178:691–696.
    OpenUrlAbstract/FREE Full Text
PreviousNext
Back to top
Download PDF
Citation Tools
Complete Genome Sequences of Azotobacter vinelandii Wild-Type Strain CA and Tungsten-Tolerant Mutant Strain CA6
Jesse D. Noar, José M. Bruno-Bárcena
Genome Announcements Jun 2013, 1 (3) e00313-13; DOI: 10.1128/genomeA.00313-13

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print
Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Microbiology Resource Announcements article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Complete Genome Sequences of Azotobacter vinelandii Wild-Type Strain CA and Tungsten-Tolerant Mutant Strain CA6
(Your Name) has forwarded a page to you from Microbiology Resource Announcements
(Your Name) thought you would be interested in this article in Microbiology Resource Announcements.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Complete Genome Sequences of Azotobacter vinelandii Wild-Type Strain CA and Tungsten-Tolerant Mutant Strain CA6
Jesse D. Noar, José M. Bruno-Bárcena
Genome Announcements Jun 2013, 1 (3) e00313-13; DOI: 10.1128/genomeA.00313-13
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • GENOME ANNOUNCEMENT
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Info & Metrics
  • PDF

Related Articles

Cited By...

About

  • About MRA
  • Editor in Chief
  • Board of Editors
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • Getting Started
  • Submit a Manuscript
  • Author Warranty
  • Ethics
  • Contact Us
  • ASM Author Center

Follow #MRAJournal

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Online ISSN: 2576-098X