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  • Home
  • Articles
    • Latest Articles
    • Archive
  • Types of Resources
    • Amplicon Sequence Collections
    • Culture Collections/Mutant Libraries
    • Databases and Software
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  • For Authors
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Other Genetic Resources

  • Open Access
    Deeply Sequenced Infectious Clones of Key Cassava Begomovirus Isolates from Cameroon
    Other Genetic Resources
    Deeply Sequenced Infectious Clones of Key Cassava Begomovirus Isolates from Cameroon

    We deeply sequenced two pairs of widely used infectious clones (4 plasmids) of the bipartite begomoviruses African cassava mosaic virus (ACMV) and East African cassava mosaic Cameroon virus (EACMCV). The ACMV clones were quite divergent from published sequences. Raw reads, consensus plasmid sequences, and the infectious clones themselves are all publicly available.

    J. Steen Hoyer, Vincent N. Fondong, Mary M. Dallas, Catherine Doyle Aimone, David O. Deppong, Siobain Duffy, Linda Hanley-Bowdoin
  • Open Access
    pSETT4, an Improved φC31-Based Integrative Vector System for <em>Actinoplanes</em> sp. SE50/110
    Other Genetic Resources
    pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110

    The pSETT4 vector integrates into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transcription. The system can be used in other Actinomycetales by exchanging the promoter.

    Lena Schaffert, Lucas Jacob, Susanne Schneiker-Bekel, Marcus Persicke, Camilla März, Christian Rückert, Alfred Pühler, Jörn Kalinowski
  • Open Access
    Plasmid Sequences of Four Large Plasmids Carrying Antimicrobial Resistance Genes in <span class="named-content genus-species" id="named-content-1">Escherichia coli</span> Strains Isolated from Beef Cattle in Japan
    Other Genetic Resources
    Plasmid Sequences of Four Large Plasmids Carrying Antimicrobial Resistance Genes in Escherichia coli Strains Isolated from Beef Cattle in Japan

    Escherichia coli is a common reservoir for antimicrobial resistance genes that can be easily transformed to possess multidrug resistance through plasmid transfer. To understand multidrug resistance plasmids, we report the plasmid sequences of four large plasmids carrying a number of genes related to antimicrobial resistance that were found in...

    Shiori Yamamoto, Wataru Kitagawa, Motoki Nakano, Hiroshi Asakura, Eriko Iwabuchi, Teruo Sone, Kozo Asano
  • Open Access
    A New <span class="named-content genus-species" id="named-content-1">Escherichia coli</span> Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes
    Other Genetic Resources
    A New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes

    A series of new Escherichia coli entry vectors (pIIS18-SapI, pIIS18-BsmBI, pIIS18-BsaI, pIIS18-BfuAI-1, and pIIS18-BfuAI-2) was constructed based on a modified pUC18 backbone, which carried newly designed multiple cloning sites, consisting of two facing type IIS enzyme cleavage sites and one blunt-end enzyme cleavage site. These vectors are useful for seamless gene...

    Hend Altaib, Yuka Ozaki, Tomoya Kozakai, Yassien Badr, Izumi Nomura, Tohru Suzuki
  • Open Access
    Broad-Host-Range Plasmids for Constitutive and Inducible Gene Expression in the Absence of Antibiotic Selection
    Other Genetic Resources
    Broad-Host-Range Plasmids for Constitutive and Inducible Gene Expression in the Absence of Antibiotic Selection

    Plasmid vectors are a valuable research tool for characterizing bacterial gene function, but there is a limited range of plasmids that are functional in nonmodel bacterial species. Described here is a set of broad-host-range plasmids modified for stability in the absence of antibiotic selection and for gene expression manipulation.

    Lindsey Burbank, Wei Wei
  • Open Access
    Full Genome Sequence of pT3, a Multiresistant Plasmid Carrying the <em>mcr-3.5</em> Colistin Resistance Gene, Recovered from an Extended-Spectrum-β-Lactamase-Producing <span class="named-content genus-species" id="named-content-1">Escherichia coli</span> Isolate from Crickets Sold as Food
    Other Genetic Resources
    Full Genome Sequence of pT3, a Multiresistant Plasmid Carrying the mcr-3.5 Colistin Resistance Gene, Recovered from an Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolate from Crickets Sold as Food

    Here, we sequenced multidrug resistance plasmid pT3, which encodes mcr-3.5 and other resistance genes. The cooccurrence of mcr genes and other resistance genes on a single plasmid is of concern.

    Katrin Zurfluh, Marc J. A. Stevens, Melissa Bucher, Laurent Poirel, Patrice Nordmann, Roger Stephan
  • Open Access
    Complete Sequences of Two Plasmids Found in a Brazilian <span class="named-content genus-species" id="named-content-1">Bacillus thuringiensis</span> Serovar israelensis Strain
    Other Genetic Resources
    Complete Sequences of Two Plasmids Found in a Brazilian Bacillus thuringiensis Serovar israelensis Strain

    Plasmids play a crucial role in the evolution of bacterial genomes by mediating horizontal gene transfer. In this work, we sequenced two plasmids found in a Brazilian Bacillus thuringiensis serovar israelensis strain which showed 100% nucleotide identities with Bacillus thuringiensis serovar...

    Fabrício S. Campos, Fernando B. Cerqueira, Gil R. Santos, Eliseu J. G. Pereira, Roberto F. T. Corrêia, Alex S. R. Cangussu, Fernando L. Melo, Bergmann M. Ribeiro, Raimundo W. S. Aguiar
  • Open Access
    Sequence Variation in Multidrug-Resistant Plasmid pLUH01, Isolated from Human Nasopharyngeal Swabs
    Other Genetic Resources
    Sequence Variation in Multidrug-Resistant Plasmid pLUH01, Isolated from Human Nasopharyngeal Swabs

    Three variants of the multidrug-resistant plasmid pLUH01 were assembled by deep sequencing from nasopharyngeal swabs. All have a 21-bp deletion in the RS14515 hypothetical gene.

    Kate V. Atkinson, Lisa A. Bishop, Glenn Rhodes, Nicolas Salez, Neil R. McEwan, Matthew J. Hegarty, Julie Robey, Nicola Harding, Simon Wetherell, Robert M. Lauder, Roger W. Pickup, Mark Wilkinson, Derek Gatherer
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