Other Genetic Resources
Other Genetic ResourcespSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110The pSETT4 vector integrates into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transcription. The system can be used in other Actinomycetales by exchanging the promoter.
Other Genetic ResourcesPlasmid Sequences of Four Large Plasmids Carrying Antimicrobial Resistance Genes in Escherichia coli Strains Isolated from Beef Cattle in JapanEscherichia coli is a common reservoir for antimicrobial resistance genes that can be easily transformed to possess multidrug resistance through plasmid transfer. To understand multidrug resistance plasmids, we report the plasmid sequences of four large plasmids carrying a number of genes related to antimicrobial resistance that were found in...
Other Genetic ResourcesA New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction EnzymesA series of new Escherichia coli entry vectors (pIIS18-SapI, pIIS18-BsmBI, pIIS18-BsaI, pIIS18-BfuAI-1, and pIIS18-BfuAI-2) was constructed based on a modified pUC18 backbone, which carried newly designed multiple cloning sites, consisting of two facing type IIS enzyme cleavage sites and one blunt-end enzyme cleavage site. These vectors are useful for seamless gene...
Other Genetic ResourcesBroad-Host-Range Plasmids for Constitutive and Inducible Gene Expression in the Absence of Antibiotic SelectionPlasmid vectors are a valuable research tool for characterizing bacterial gene function, but there is a limited range of plasmids that are functional in nonmodel bacterial species. Described here is a set of broad-host-range plasmids modified for stability in the absence of antibiotic selection and for gene expression manipulation.
Other Genetic ResourcesFull Genome Sequence of pT3, a Multiresistant Plasmid Carrying the mcr-3.5 Colistin Resistance Gene, Recovered from an Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolate from Crickets Sold as FoodHere, we sequenced multidrug resistance plasmid pT3, which encodes mcr-3.5 and other resistance genes. The cooccurrence of mcr genes and other resistance genes on a single plasmid is of concern.
Other Genetic ResourcesComplete Sequences of Two Plasmids Found in a Brazilian Bacillus thuringiensis Serovar israelensis StrainPlasmids play a crucial role in the evolution of bacterial genomes by mediating horizontal gene transfer. In this work, we sequenced two plasmids found in a Brazilian Bacillus thuringiensis serovar israelensis strain which showed 100% nucleotide identities with Bacillus thuringiensis serovar...
Other Genetic ResourcesSequence Variation in Multidrug-Resistant Plasmid pLUH01, Isolated from Human Nasopharyngeal SwabsThree variants of the multidrug-resistant plasmid pLUH01 were assembled by deep sequencing from nasopharyngeal swabs. All have a 21-bp deletion in the RS14515 hypothetical gene.