Other Genetic Resources
Other Genetic ResourcesA New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction EnzymesA series of new Escherichia coli entry vectors (pIIS18-SapI, pIIS18-BsmBI, pIIS18-BsaI, pIIS18-BfuAI-1, and pIIS18-BfuAI-2) was constructed based on a modified pUC18 backbone, which carried newly designed multiple cloning sites, consisting of two facing type IIS enzyme cleavage sites and one blunt-end enzyme cleavage site. These vectors are useful for seamless gene...
Other Genetic ResourcesBroad-Host-Range Plasmids for Constitutive and Inducible Gene Expression in the Absence of Antibiotic SelectionPlasmid vectors are a valuable research tool for characterizing bacterial gene function, but there is a limited range of plasmids that are functional in nonmodel bacterial species. Described here is a set of broad-host-range plasmids modified for stability in the absence of antibiotic selection and for gene expression manipulation.
Other Genetic ResourcesFull Genome Sequence of pT3, a Multiresistant Plasmid Carrying the mcr-3.5 Colistin Resistance Gene, Recovered from an Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolate from Crickets Sold as FoodHere, we sequenced multidrug resistance plasmid pT3, which encodes mcr-3.5 and other resistance genes. The cooccurrence of mcr genes and other resistance genes on a single plasmid is of concern.
Other Genetic ResourcesComplete Sequences of Two Plasmids Found in a Brazilian Bacillus thuringiensis Serovar israelensis StrainPlasmids play a crucial role in the evolution of bacterial genomes by mediating horizontal gene transfer. In this work, we sequenced two plasmids found in a Brazilian Bacillus thuringiensis serovar israelensis strain which showed 100% nucleotide identities with Bacillus thuringiensis serovar...
Other Genetic ResourcesSequence Variation in Multidrug-Resistant Plasmid pLUH01, Isolated from Human Nasopharyngeal SwabsThree variants of the multidrug-resistant plasmid pLUH01 were assembled by deep sequencing from nasopharyngeal swabs. All have a 21-bp deletion in the RS14515 hypothetical gene.