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  • Home
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  • Types of Resources
    • Amplicon Sequence Collections
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Culture Collections/Mutant Libraries

  • Open Access
    Characterization of <span class="named-content genus-species" id="named-content-1">Clostridioides difficile</span> Isolates Available through the CDC &amp; FDA Antibiotic Resistance Isolate Bank
    Culture Collections/Mutant Libraries
    Characterization of Clostridioides difficile Isolates Available through the CDC & FDA Antibiotic Resistance Isolate Bank

    Thirty Clostridioides difficile isolates collected in 2016 through the Centers for Disease Control and Prevention Emerging Infections Program were selected for reference antimicrobial susceptibility testing and whole-genome sequencing. Here, we present the genetic characteristics of these isolates and announce their availability in the CDC & FDA Antibiotic...

    Ashley Paulick, Michelle Adamczyk, Karen Anderson, Nick Vlachos, María-José Machado, Gillian McAllister, Lauren Korhonen, Alice Y. Guh, Alison Laufer Halpin, J. Kamile Rasheed, Maria Karlsson, Joseph D. Lutgring, Amy S. Gargis, the Emerging Infections Program Clostridioides difficile Pathogen Group
  • Open Access
    A Culture Collection of 50 <span class="named-content genus-species" id="named-content-1">Neisseria gonorrhoeae</span> Isolates
    Culture Collections/Mutant Libraries
    A Culture Collection of 50 Neisseria gonorrhoeae Isolates

    A culture collection of 50 Neisseria gonorrhoeae isolates is available from the CDC & FDA Antibiotic Resistance Isolate Bank. Associated data include antibiotic susceptibility information for azithromycin, cefixime, cefpodoxime, ceftriaxone, tetracycline, ciprofloxacin, penicillin, and spectinomycin and linked whole-genome sequences.

    H. Liu, E. Vidyaprakash, M. W. Schmerer, D. C. Pham, S. St Cyr, E. N. Kersh
  • Open Access
    Announcing the Availability of a Culture Collection of Uranium-Resistant Microbial Assemblages (CURMA) Obtained from Metalliferous Soils of the Savannah River Site, USA
    Culture Collections/Mutant Libraries
    Announcing the Availability of a Culture Collection of Uranium-Resistant Microbial Assemblages (CURMA) Obtained from Metalliferous Soils of the Savannah River Site, USA

    Metagenomic assessment provides a comprehensive survey of soil microbiota; however, isolation and characterization of functionally relevant microbiota are required prior to their application(s), such as for metal remediation. Toward this end, we report the availability of a culture collection comprising uranium (U)-resistant microbial assemblages (CURMA) to the scientific community.

    Meenakshi Agarwal, Rajesh Singh Rathore, Allen Black, Xiaoyu Xu, John Seaman, Ashvini Chauhan
  • Open Access
    Establishing Renal Proximal Tubule Epithelial-Derived Cell Lines Expressing Human Telomerase Reverse Transcriptase for Studying BK Polyomavirus
    Culture Collections/Mutant Libraries
    Establishing Renal Proximal Tubule Epithelial-Derived Cell Lines Expressing Human Telomerase Reverse Transcriptase for Studying BK Polyomavirus

    We previously established an infection model for BK polyomavirus (BKPyV) in primary human renal proximal tubule epithelial (RPTE) cells. Use of these cells is limited by their inability to be passaged extensively. Here, we describe RPTE cells immortalized with human telomerase reverse transcriptase (hTERT), which can serve as a model system for acute or persistent BKPyV infection.

    Linbo Zhao, Michael J. Imperiale
  • Open Access
    Collection of Recombinant Rotaviruses Expressing Fluorescent Reporter Proteins
    Culture Collections/Mutant Libraries
    Collection of Recombinant Rotaviruses Expressing Fluorescent Reporter Proteins

    A collection of recombinant rotaviruses that express the fluorescent markers UnaG, mKate, mRuby, TagBFP, CFP, or YFP as separate proteins was generated. Genes for the fluorescent proteins were inserted into genome segment 7 without compromising expression of the protein NSP3. These recombinant rotaviruses are valuable for analyzing rotavirus biology by fluorescence-based live-cell imaging.

    Asha A. Philip, Brittany E. Herrin, Maximiliano L. Garcia, Andrew T. Abad, Sarah P. Katen, John T. Patton
  • Open Access
    The Mastitis Pathogens Culture Collection
    Culture Collections/Mutant Libraries
    The Mastitis Pathogens Culture Collection

    The Mastitis Pathogen Culture Collection contains more than 16,000 mastitis-causing bacterial isolates from milk samples taken from cow quarters and bulk tanks in a national cohort of 91 dairy farms across Canada over a period of 2 years. These isolates are linked to demographic and production data that were recorded at the mammary gland, cow, and farm levels.

    Simon Dufour, Josée Labrie, Mario Jacques
  • Open Access
    First Release of the Bacterial Biobank of the Urban Environment (BBUE)
    Culture Collections/Mutant Libraries
    First Release of the Bacterial Biobank of the Urban Environment (BBUE)

    Metagenomics is providing a broad overview of bacterial functional diversity; however, culturing and biobanking are still essential for microbiology. Here, we present the Bacterial Biobank of the Urban Environment (BBUE), a sizable culture collection for long-term storage and characterization of the microbiota associated with urban environments relevant for public health.

    Verónica Antelo, Cecilia Salazar, Arací Martínez, Bruno D’Alessandro, Marta Castro, Laura Betancor, María Victoria Barcala, Diana Míguez, Gastón H. Gonnet, Gregorio Iraola
  • Open Access
    Gateway Entry Vector Library of <span class="named-content genus-species" id="named-content-1">Wolbachia pipientis</span> Candidate Effectors from Strain <em>w</em>Mel
    Culture Collections/Mutant Libraries
    Gateway Entry Vector Library of Wolbachia pipientis Candidate Effectors from Strain wMel

    Wolbachia pipientis is an intracellular symbiont that modifies host biology using a type IV secretion system to inject bacterial effectors into the host cytoplasm. We utilized a bioinformatics approach to predict Wolbachia effectors and cloned the candidates into an entry vector, which can be utilized for subsequent analyses.

    Irene L. G. Newton, Kathy B. Sheehan
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