Culture Collections/Mutant Libraries
- Culture Collections/Mutant LibrariesDeletion Mutants, Archived Transposon Library, and Tagged Protein Constructs of the Model Sulfate-Reducing Bacterium Desulfovibrio vulgaris Hildenborough
The dissimilatory sulfate-reducing deltaproteobacterium Desulfovibrio vulgaris Hildenborough (ATCC 29579) was chosen by the research collaboration ENIGMA to explore tools and protocols for bringing this anaerobe to model status. Here, we describe a collection of genetic constructs generated by ENIGMA that are available to the research community.
Culture Collections/Mutant LibrariesCharacterization of Clostridioides difficile Isolates Available through the CDC & FDA Antibiotic Resistance Isolate BankThirty Clostridioides difficile isolates collected in 2016 through the Centers for Disease Control and Prevention Emerging Infections Program were selected for reference antimicrobial susceptibility testing and whole-genome sequencing. Here, we present the genetic characteristics of these isolates and announce their availability in the CDC & FDA Antibiotic...
Culture Collections/Mutant LibrariesA Culture Collection of 50 Neisseria gonorrhoeae IsolatesA culture collection of 50 Neisseria gonorrhoeae isolates is available from the CDC & FDA Antibiotic Resistance Isolate Bank. Associated data include antibiotic susceptibility information for azithromycin, cefixime, cefpodoxime, ceftriaxone, tetracycline, ciprofloxacin, penicillin, and spectinomycin and linked whole-genome sequences.
Culture Collections/Mutant LibrariesAnnouncing the Availability of a Culture Collection of Uranium-Resistant Microbial Assemblages (CURMA) Obtained from Metalliferous Soils of the Savannah River Site, USAMetagenomic assessment provides a comprehensive survey of soil microbiota; however, isolation and characterization of functionally relevant microbiota are required prior to their application(s), such as for metal remediation. Toward this end, we report the availability of a culture collection comprising uranium (U)-resistant microbial assemblages (CURMA) to the scientific community.
Culture Collections/Mutant LibrariesEstablishing Renal Proximal Tubule Epithelial-Derived Cell Lines Expressing Human Telomerase Reverse Transcriptase for Studying BK PolyomavirusWe previously established an infection model for BK polyomavirus (BKPyV) in primary human renal proximal tubule epithelial (RPTE) cells. Use of these cells is limited by their inability to be passaged extensively. Here, we describe RPTE cells immortalized with human telomerase reverse transcriptase (hTERT), which can serve as a model system for acute or persistent BKPyV infection.
Culture Collections/Mutant LibrariesCollection of Recombinant Rotaviruses Expressing Fluorescent Reporter ProteinsA collection of recombinant rotaviruses that express the fluorescent markers UnaG, mKate, mRuby, TagBFP, CFP, or YFP as separate proteins was generated. Genes for the fluorescent proteins were inserted into genome segment 7 without compromising expression of the protein NSP3. These recombinant rotaviruses are valuable for analyzing rotavirus biology by fluorescence-based live-cell imaging.
Culture Collections/Mutant LibrariesThe Mastitis Pathogens Culture CollectionThe Mastitis Pathogen Culture Collection contains more than 16,000 mastitis-causing bacterial isolates from milk samples taken from cow quarters and bulk tanks in a national cohort of 91 dairy farms across Canada over a period of 2 years. These isolates are linked to demographic and production data that were recorded at the mammary gland, cow, and farm levels.
Culture Collections/Mutant LibrariesFirst Release of the Bacterial Biobank of the Urban Environment (BBUE)Metagenomics is providing a broad overview of bacterial functional diversity; however, culturing and biobanking are still essential for microbiology. Here, we present the Bacterial Biobank of the Urban Environment (BBUE), a sizable culture collection for long-term storage and characterization of the microbiota associated with urban environments relevant for public health.
Culture Collections/Mutant LibrariesGateway Entry Vector Library of Wolbachia pipientis Candidate Effectors from Strain wMelWolbachia pipientis is an intracellular symbiont that modifies host biology using a type IV secretion system to inject bacterial effectors into the host cytoplasm. We utilized a bioinformatics approach to predict Wolbachia effectors and cloned the candidates into an entry vector, which can be utilized for subsequent analyses.